c7b0b77e3685ab516a02d413e9aca2c6 biomedicines_a2021v9n4p352.pdf 1e07fc6e5545dc035a6c893f1f6569255a639510 biomedicines_a2021v9n4p352.pdf 89c4aa5198f964d581c7b0f0795321e64b46be2ca466971960a954457dbc4e32 biomedicines_a2021v9n4p352.pdf Title: ZnO Nanosheet-Coated TiZrPdSiNb Alloy as a Piezoelectric Hybrid Material for Self-Stimulating Orthopedic Implants Subject: A Ti-based alloy (Ti45Zr15Pd30Si5Nb5) with already proven excellent mechanical and biocompatibility features has been coated with piezoelectric zinc oxide (ZnO) to induce the electrical self-stimulation of cells. ZnO was grown onto the pristine alloy in two different morphologies: a flat dense film and an array of nanosheets. The effect of the combined material on osteoblasts (electrically stimulable cells) was analyzed in terms of proliferation, cell adhesion, expression of differentiation markers and induction of calcium transients. Although both ZnO structures were biocompatible and did not induce inflammatory response, only the array of ZnO nanosheets was able to induce calcium transients, which improved the proliferation of Saos-2 cells and enhanced the expression of some early differentiation expression genes. The usual motion of the cells imposes strain to the ZnO nanosheets, which, in turn, create local electric fields owing to their piezoelectric character. These electric fields cause the opening of calcium voltage gates and boost cell proliferation and early differentiation. Thus, the modification of the Ti45Zr15Pd30Si5Nb5 surface with an array of ZnO nanosheets endows the alloy with smart characteristics, making it capable of electric self-stimulation. Keywords: TiZrPdSiNb alloy; piezoelectric; nanogenerators; self-stimulating; osteoblast; proliferation; differentiation Author: Oriol Careta, Jordina Fornell, Eva Pellicer, Elena Ibañez, Andreu Blanquer, Jaume Esteve, Jordi Sort, Gonzalo Murillo and Carme Nogués Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 31 03:20:52 2021 CEST ModDate: Fri Apr 9 05:17:30 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3992676 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BTFGED+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 MWHIBA+VnURWPalladioL Type 1 Custom yes yes no 15 0 JBMNRG+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 PKWINC+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 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283 0 BAEJOG+PalatinoLinotype TrueType WinAnsi yes yes no 286 0 BAEJPI+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 289 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:51:53 CEST RepresentationInformation: biomedicines_a2021v9n4p352.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 11:50:52 CEST Size: 3992676 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 517 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Material Fabrication Destination: subsection.2.1 Item: Title: Structural Characterization Destination: subsection.2.2 Item: Title: Cell Culture Destination: subsection.2.3 Item: Title: Quantification of Cell Proliferation Destination: subsection.2.4 Item: Title: Quantification of Inflammatory Cytokines Secretion Destination: subsection.2.5 Item: Title: Cell Adhesion Analysis Destination: subsection.2.6 Item: Title: Cell Morphology Analysis Destination: subsection.2.7 Item: Title: Intracellular Calcium Measurement Destination: subsection.2.8 Item: Title: Quantitative Real-Time PCR Destination: subsection.2.9 Item: Title: Statistical Analysis Destination: subsection.2.10 Item: Title: Results Destination: section.3 Children: Item: Title: Morphology, Composition and Structure of the Samples Destination: subsection.3.1 Item: Title: Cell Proliferation Destination: subsection.3.2 Item: Title: Induction of Inflammatory Cytokine Secretion Destination: subsection.3.3 Item: Title: Cell Morphology and Adhesion Analysis Destination: subsection.3.4 Item: Title: Induction of Intracellular Calcium Transients Destination: subsection.3.5 Item: Title: Expression of Osteogenic Markers Destination: subsection.3.6 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: ZnO Nanosheet-Coated TiZrPdSiNb Alloy as a Piezoelectric Hybrid Material for Self-Stimulating Orthopedic Implants Author: Oriol Careta, Jordina Fornell, Eva Pellicer, Elena Ibañez, Andreu Blanquer, Jaume Esteve, Jordi Sort, Gonzalo Murillo and Carme Nogués Subject: A Ti-based alloy (Ti45Zr15Pd30Si5Nb5) with already proven excellent mechanical and biocompatibility features has been coated with piezoelectric zinc oxide (ZnO) to induce the electrical self-stimulation of cells. ZnO was grown onto the pristine alloy in two different morphologies: a flat dense film and an array of nanosheets. The effect of the combined material on osteoblasts (electrically stimulable cells) was analyzed in terms of proliferation, cell adhesion, expression of differentiation markers and induction of calcium transients. Although both ZnO structures were biocompatible and did not induce inflammatory response, only the array of ZnO nanosheets was able to induce calcium transients, which improved the proliferation of Saos-2 cells and enhanced the expression of some early differentiation expression genes. The usual motion of the cells imposes strain to the ZnO nanosheets, which, in turn, create local electric fields owing to their piezoelectric character. These electric fields cause the opening of calcium voltage gates and boost cell proliferation and early differentiation. Thus, the modification of the Ti45Zr15Pd30Si5Nb5 surface with an array of ZnO nanosheets endows the alloy with smart characteristics, making it capable of electric self-stimulation. Keywords: TiZrPdSiNb alloy; piezoelectric; nanogenerators; self-stimulating; osteoblast; proliferation; differentiation Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 31 03:20:52 CEST 2021 ModDate: Fri Apr 09 05:17:30 CEST 2021 ID: 0x8651dc0b88ef396e1f159cd10450c15e, 0x8651dc0b88ef396e1f159cd10450c15e Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: EOLEFD+CMSY10 FontSubset: true FirstChar: 1 LastChar: 14 FontDescriptor: FontName: EOLEFD+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: JBMNRG+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: JBMNRG+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MPUDFL+PazoMath FontSubset: true FirstChar: 68 LastChar: 68 FontDescriptor: FontName: MPUDFL+PazoMath Flags: Symbolic FontBBox: -40, -283, 878, 946 FontFile: true Font: BaseFont: 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