5f6efb34260227ab3fbb02fbeb0a8514 animals_a2021v11n4p1079.pdf 238b874325314bce34c5d0281249d5501aab0db8 animals_a2021v11n4p1079.pdf a3f578dc2733d510899794d4a554f5713084747af8edc6c3592207c1726b42c8 animals_a2021v11n4p1079.pdf Title: Social Nesting, Animal Welfare, and Disease Monitoring Subject: The assessment of welfare and disease progression in animal models is critical. Most tools rely on evaluating individual subjects, whereas social behaviors, also sensitive to acute illness, chronic diseases, or mental health, are scarcely monitored because they are complex and time-consuming. We propose the evaluation of social nesting, a species-typical behavior naturally occurring in standard housing conditions, for such behavioral monitoring. We provide an example of its use to evaluate social deficits and the long-term effects of neonatal tactile-proprioceptive sensorial stimulation from postnatal day 1 to 21, in male and female adult 3xTg-AD mice for Alzheimer’s disease compared to sex- and age-matched non-transgenic (NTg) counterparts with normal aging. Social nesting was sensitive to genotype (worse in 3xTg-AD mice), sex (worse in males), profile, and treatment (distinct time to observe the maximum score and incidence of the perfect nest). Since social nesting can be easily included in housing routines, this neuroethological approach can be useful for animal welfare, monitoring the disease’s progress, and evaluating potential risk factors and effects of preventive/therapeutical strategies. Finally, the noninvasive, painless, simple, short time, and low-cost features of this home-cage monitoring are advantages that make social nesting feasible to be successfully implemented in most animal department settings. Keywords: nest-building; social behavior; behavioral monitoring; animal welfare; 3xTg-AD mice; Alzheimer’s disease; gender medicine; early-life events; early-life interventions; long-term effects Author: Lydia Giménez-Llort and Virginia Torres-Lista Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Apr 12 10:42:24 2021 CEST ModDate: Mon Apr 12 11:02:36 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1438216 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HCBGQO+VnURWPalladioL Type 1 Custom yes yes no 10 0 NAHNLH+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 QPHQXF+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 BBXTKI+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 BTFGED+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 VYVZGZ+CMSY10 Type 1 Builtin yes yes no 78 0 JLOABN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 92 0 JLOABP+PalatinoLinotype TrueType WinAnsi yes yes no 95 0 JLOACB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 98 0 JLOBIJ+TimesNewRoman CID TrueType Identity-H yes yes yes 101 0 JLOABN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 119 0 JLOABP+PalatinoLinotype TrueType WinAnsi yes yes no 122 0 JLOACB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 125 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-04-20 02:46:41 CEST RepresentationInformation: animals_a2021v11n4p1079.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-04-14 12:28:45 CEST Size: 1438216 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 269 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Subjects Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Early-Life Intervention: Neonatal Handling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Behavioral Assessments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Statistics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Discussion and Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Info: Title: Social Nesting, Animal Welfare, and Disease Monitoring Author: Lydia Giménez-Llort and Virginia Torres-Lista Subject: The assessment of welfare and disease progression in animal models is critical. Most tools rely on evaluating individual subjects, whereas social behaviors, also sensitive to acute illness, chronic diseases, or mental health, are scarcely monitored because they are complex and time-consuming. We propose the evaluation of social nesting, a species-typical behavior naturally occurring in standard housing conditions, for such behavioral monitoring. We provide an example of its use to evaluate social deficits and the long-term effects of neonatal tactile-proprioceptive sensorial stimulation from postnatal day 1 to 21, in male and female adult 3xTg-AD mice for Alzheimer’s disease compared to sex- and age-matched non-transgenic (NTg) counterparts with normal aging. Social nesting was sensitive to genotype (worse in 3xTg-AD mice), sex (worse in males), profile, and treatment (distinct time to observe the maximum score and incidence of the perfect nest). Since social nesting can be easily included in housing routines, this neuroethological approach can be useful for animal welfare, monitoring the disease’s progress, and evaluating potential risk factors and effects of preventive/therapeutical strategies. Finally, the noninvasive, painless, simple, short time, and low-cost features of this home-cage monitoring are advantages that make social nesting feasible to be successfully implemented in most animal department settings. Keywords: nest-building; social behavior; behavioral monitoring; animal welfare; 3xTg-AD mice; Alzheimer’s disease; gender medicine; early-life events; early-life interventions; long-term effects Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Apr 12 10:42:24 CEST 2021 ModDate: Mon Apr 12 11:02:36 CEST 2021 ID: 0x7467cb42407e0123e4971dae997c6387, 0x7467cb42407e0123e4971dae997c6387 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QPHQXF+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: QPHQXF+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: BBXTKI+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 241 FontDescriptor: FontName: BBXTKI+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HCBGQO+VnURWPalladioL FontSubset: true FirstChar: 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