934448061ec549ec9b9403af5416f4bd fcell_a2021v9m4p669182.pdf 0caef9762ba0f1f2f454fc5641fcaaf2445b7531 fcell_a2021v9m4p669182.pdf 667651f7eafe51bd2cc198e70219bf6fc69f1ec797aea5f7bd477d184c72cc5e fcell_a2021v9m4p669182.pdf Title: Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements Subject: Sperm present a highly particular DNA condensation that is acquired during their differentiation. Keywords: sperm, DNA condensation, DNA damage, protamine, mammals Author: Jordi Ribas-Maynou Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Mon Apr 26 12:20:50 2021 CEST ModDate: Mon Apr 26 12:20:50 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 2002491 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TKUFML+MinionPro-Regular Type 1 Custom yes yes no 25 0 UOYJUS+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 26 0 IIXPCE+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 27 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 32 0 BYOWQV+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 33 0 NRTWIL+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 34 0 MBOWKV+MTSYN Type 1 Builtin yes yes no 35 0 SQRAFJ+RBLMI Type 1 Builtin yes yes no 36 0 HFTDFW+MinionPro-It Type 1 Custom yes yes no 176 0 YVUSLV+HelveticaNeueLTStd-Md Type 1 Custom yes yes no 261 0 EWYLZG+MTGU Type 1 Builtin yes yes no 265 0 REXZNZ+MinionPro-Bold Type 1 Custom yes yes no 330 0 GYWJSV+MTSYB Type 1 Builtin yes yes no 400 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-06-15 02:36:15 CEST RepresentationInformation: fcell_a2021v9m4p669182.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-06-14 12:28:32 CEST Size: 2002491 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 828 FreeObjects: 4 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Reagents Item: Title: Sperm Sample Obtaining and Cryopreservation Children: Item: Title: Human Sperm Item: Title: Pig Sperm Item: Title: Horse Sperm Item: Title: Donkey Sperm Item: Title: Cattle Sperm Item: Title: Halo Formation Item: Title: Assessment of DNA Decondensation Item: Title: Staining of Nuclear DNA in Non-decondensed Sperm Cells Item: Title: Statistics Item: Title: Results Children: Item: Title: Lysis Solutions Used for Halo Formation Induce Nuclear Core Decondensation Item: Title: Prolonged Lysis Incubation Contributes to Higher Efficiency in Protamine Removal Item: Title: The Halo/Core Ratio Reflects the Decondensation Status of a Sperm DNA Item: Title: The Inclusion of Proteinase K in the Third Lysis Solution Accelerates DNA Decondensation Item: Title: Discussion Item: Title: Conclusion Item: Title: Data Availability Statement Item: Title: Ethics Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Supplementary Material Item: Title: References Info: Title: Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements Author: Jordi Ribas-Maynou Subject: Sperm present a highly particular DNA condensation that is acquired during their differentiation. Keywords: sperm, DNA condensation, DNA damage, protamine, mammals Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Mon Apr 26 12:50:50 CEST 2021 ModDate: Mon Apr 26 12:50:50 CEST 2021 ID: 0xb100710d8e14aec9bd877c0e4fb018d3, 0xb100710d8e14aec9bd877c0e4fb018d3 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: CCVXXJ+HelveticaNeueLTStd-BdIt FontSubset: true FirstChar: 42 LastChar: 121 FontDescriptor: FontName: CCVXXJ+HelveticaNeueLTStd-BdIt Flags: Symbolic FontBBox: -166, -218, 1129, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: BYOWQV+HelveticaNeueLTStd-LtIt FontSubset: true FirstChar: 2 LastChar: 243 FontDescriptor: FontName: BYOWQV+HelveticaNeueLTStd-LtIt Flags: Symbolic FontBBox: -165, -214, 1099, 951 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: NRTWIL+HelveticaNeueLTStd-MdIt FontSubset: true FirstChar: 42 LastChar: 243 FontDescriptor: FontName: NRTWIL+HelveticaNeueLTStd-MdIt Flags: Symbolic FontBBox: -165, -218, 1125, 947 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: MBOWKV+MTSYN FontSubset: true FirstChar: 0 LastChar: 112 FontDescriptor: FontName: MBOWKV+MTSYN Flags: Symbolic FontBBox: 0, -954, 1043, 900 FontFile: true Font: BaseFont: SQRAFJ+RBLMI FontSubset: true FirstChar: 59 LastChar: 62 FontDescriptor: FontName: SQRAFJ+RBLMI Flags: Symbolic FontBBox: -11, -230, 987, 750 FontFile: true Font: BaseFont: YVUSLV+HelveticaNeueLTStd-Md FontSubset: true FirstChar: 67 LastChar: 121 FontDescriptor: FontName: YVUSLV+HelveticaNeueLTStd-Md Flags: Symbolic FontBBox: -165, -221, 1066, 952 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EWYLZG+MTGU FontSubset: true FirstChar: 98 LastChar: 109 FontDescriptor: FontName: EWYLZG+MTGU Flags: Symbolic FontBBox: 25, -213, 839, 683 FontFile: true Font: BaseFont: REXZNZ+MinionPro-Bold FontSubset: true FirstChar: 3 LastChar: 121 FontDescriptor: FontName: REXZNZ+MinionPro-Bold Flags: Symbolic FontBBox: -318, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HFTDFW+MinionPro-It FontSubset: true FirstChar: 40 LastChar: 243 FontDescriptor: FontName: HFTDFW+MinionPro-It Flags: Symbolic FontBBox: -201, -360, 1684, 919 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GYWJSV+MTSYB FontSubset: true FirstChar: 0 LastChar: 67 FontDescriptor: FontName: GYWJSV+MTSYB Flags: Symbolic FontBBox: -16, -935, 1043, 896 FontFile: true Font: BaseFont: TKUFML+MinionPro-Regular FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: TKUFML+MinionPro-Regular Flags: Symbolic FontBBox: -289, -360, 1684, 917 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: UOYJUS+HelveticaNeueLTStd-Bd FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: UOYJUS+HelveticaNeueLTStd-Bd Flags: Symbolic FontBBox: -166, -218, 1078, 975 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: IIXPCE+HelveticaNeueLTStd-Lt FontSubset: true FirstChar: 2 LastChar: 124 FontDescriptor: FontName: IIXPCE+HelveticaNeueLTStd-Lt Flags: Symbolic FontBBox: -166, -214, 1050, 967 FontFile: true EncodingDictionary: Differences: true XMP: sperm, DNA condensation, DNA damage, protamine, mammals pdfTeX-1.40.15 1.5 application/pdf Species-Specific Differences in Sperm Chromatin Decondensation Between Eutherian Mammals Underlie Distinct Lysis Requirements Sperm present a highly particular DNA condensation that is acquired during their differentiation. 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