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Title:          \advance \chk@titlecnt \@ne Multifunctional antibody-conjugated coiled-coil protein nanoparticles for selective cell targeting\global \chk@titlecnt =\z@ 
Subject:        Acta Biomaterialia, 131 (2021) 472-482. doi:10.1016/j.actbio.2021.06.040
Keywords:       "Functional inclusion bodies"; "Coiled-coil"; "Protein nanoparticles"; "Antibody"; "Cell targeting"
Author:         Marcos Gil-Garcia
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      Title: \advance \chk@titlecnt \@ne Multifunctional antibody-conjugated coiled-coil protein nanoparticles for selective cell targeting\global \chk@titlecnt =\z@ 
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Protein production and purification
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          Title: 2.2 Cell fractionation
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          Title: 2.3 Preparation of antibody-loaded IBs
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         Item: 
          Title: 2.4 Stability in bovine serum
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         Item: 
          Title: 2.5 Epifluorescence microscopy
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         Item: 
          Title: 2.6 Confocal microscopy
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         Item: 
          Title: 2.7 Fourier transform infrared spectroscopy (FTIR)
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         Item: 
          Title: 2.8 Circular dichroism (CD) spectroscopy
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          Title: 2.9 Scanning electron microscopy (SEM) and dynamic light scattering (DLS)
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          Title: 2.10 Congo red (CR) binding assay
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          Title: 2.11 Fluorescence spectra
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          Title: 2.14 Specific cell targeting by decorated IBs
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          Title: 2.15 Flow cytometry assay
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        Title: 3 Results
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          Title: 3.1 ZapB-GFP-Z forms nonamyloid -helix-rich IBs
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         Item: 
          Title: 3.2 ZapB-GFP-Z IBs are bifunctional
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          Title: 3.3 ZapB-GFP-Z IBs are biocompatible
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         Item: 
          Title: 3.4 Antibody-decorated ZapB-GFP-Z IBs specifically target human cancer cells
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          Title: 3.5 Dual antibody binding by the ZapB-GFP-Z IBs
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          Title: 3.6 Dual antibody-conjugated ZapB-GFP-Z IBs direct T lymphocytes to HeLa cells
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        Title: 4 Discussion
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        Title: 5 Conclusion
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        Title: Funding
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        Title: Declaration of Competing Interest
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        Title: Acknowledgments
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        Title: Supplementary materials
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        Title: References
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   Type: CRC32
  Checksum: 079b4a249122059a58f5f54df8ce8aba
   Type: MD5
  Checksum: 9ef50aafd476c33bf6a86e3ce9cbf67cf47d8ff7
   Type: SHA-1