dca7e1ff48c69c57e928b02a46e6daec 255666.pdf 3c717763690dada1094c5fc3013541fdf901aa44 255666.pdf 5881fa0b47217d8e6880e23ac24749dfbe9738f7fa9743a13a404af51aab8190 255666.pdf Title: Reference Intervals of Thyroid Function Tests Assessed by Immunoassay and Mass Spectrometry in Healthy Pregnant Women Living in Catalonia Subject: Background: Recent guidelines recommend establishing a local reference interval (RI) for thyroid function. We aimed to establish trimester-specific RIs for thyrotropin (TSH) and free thyroxine (FT4) in a cohort of healthy pregnant women in Catalonia (Spain). Methods: A prospective observational study was conducted with 332 healthy pregnant women, from the first trimester (1T) to delivery. TSH was measured using an Architect® immunoassay (Abbott) and FT4 by two immunoassays, Architect® (Abbott) and Cobas® (Roche), in the three trimesters. FT4 was also measured by liquid chromatography mass spectrometry (LC/MS/MS) in the 1T. Results: TSH (UI/mL) increased throughout pregnancy (1T: 0.03–3.78; 2T: 0.51–3.53; 3T: 0.50–4.32; p < 0.0001) and FT4 (pmol/L) progressively decreased (Architect® 1T: 10.42–15.96; 2T: 8.37–12.74; 3T: 8.24–12.49; p < 0.0001; and Cobas®: 1T: 11.46–19.05; 2T: 9.65–14.67; 3T: 8.88–14.54; p < 0.0067). The FT4 RI during 1T determined LC/MS/MS was 8.75–18.27. Despite the 1T FT4 results measured by LC/MS/MS and with the two immunoassays being significantly correlated, the results obtained by the three methods were found to be non-interchangeable. Conclusions: We established trimester-specific RIs for TSH and for FT4 with immunoassays in our population. We also validated the 1T FT4 using LC/MS/MS to confirm the results of FT4 lower than the 2.5th percentile or higher than the 97.5th percentile. Keywords: thyroid function; pregnancy; reference intervals; immunoassays; tandem mass Author: José María Hernández, Berta Soldevila, Inés Velasco, Fernando Moreno-Flores, Laura Ferrer, Alejandra Pérez-Montes de Oca, Cecilia Santillán, Carla Muñoz, Sílvia Ballesta, Cristina Canal, Manel Puig-Domingo and María Luisa Granada Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Jun 1 04:14:01 2021 CEST ModDate: Tue Jun 1 04:14:01 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1134417 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TQJYPB+URWPalladioL-Ital Type 1 Custom yes yes no 100 0 CNYIIQ+URWPalladioL-Bold Type 1 Custom yes yes no 101 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 102 0 PCIAQE+URWPalladioL-Roma Type 1 Custom 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がどぱふどつぜ〴ぜ〰ぃとひはねちぴはでひちばとべ〭きちびびぜ〴ぜ〰こばづっぴひはねづぴひべぜ〴ぜ〰ぜ〵ぜ〰がぃ〯きこ〯きこぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸱ Children: Item: Title: ぃとづねどっちぬび〬ぜ〴ぜ〰げづちでづのぴび〬ぜ〴ぜ〰ちのつぜ〴ぜ〰こぴちのつちひつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸲ Item: Title: こはぬふぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰こぴちのつちひつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸳ Item: Title: こちねばぬづぜ〴ぜ〰ぐひづばちひちぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸳⸴ Item: Title: がぃ〯きこ〯きこぜ〴ぜ〰こづぴふばぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰こぴちぴどびぴどっびぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: こぴちぴどびぴどっびぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸵ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: QLBIEB+URWPalladioL-BoldItal FontSubset: true FirstChar: 110 LastChar: 112 FontDescriptor: FontName: QLBIEB+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TQJYPB+URWPalladioL-Ital FontSubset: true FirstChar: 40 LastChar: 122 FontDescriptor: FontName: 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