e6352352a5b3ee28f63658988c99b9d8 Vir_a2021v13n5p885iENG.pdf 9baa247c82e71430799c67a33aba76e9f26cb28a Vir_a2021v13n5p885iENG.pdf a2ce12ee2fc3f9b6e527dce38117888de8f117b99ea8669094fa3ffc70d8a470 Vir_a2021v13n5p885iENG.pdf Title: A Replicating Viral Vector Greatly Enhances Accumulation of Helical Virus-Like Particles in Plants Subject: The production of plant helical virus-like particles (VLPs) via plant-based expression has been problematic with previous studies suggesting that an RNA scaffold may be necessary for their efficient production. To examine this, we compared the accumulation of VLPs from two potexviruses, papaya mosaic virus and alternanthera mosaic virus (AltMV), when the coat proteins were expressed from a replicating potato virus X- based vector (pEff) and a non-replicating vector (pEAQ-HT). Significantly greater quantities of VLPs could be purified when pEff was used. The pEff system was also very efficient at producing VLPs of helical viruses from different virus families. Examination of the RNA content of AltMV and tobacco mosaic virus VLPs produced from pEff revealed the presence of vector-derived RNA sequences, suggesting that the replicating RNA acts as a scaffold for VLP assembly. Cryo-EM analysis of the AltMV VLPs showed they had a structure very similar to that of authentic potexvirus particles. Thus, we conclude that vectors generating replicating forms of RNA, such as pEff, are very efficient for producing helical VLPs. Keywords: virus-like particle; overexpression; filamentous; rod-shaped; pEAQ-HT; pEff; Potexvirus; Potyviridae; high-resolution cryo-EM Author: Eva C. Thuenemann, Matthew J. Byrne, Hadrien Peyret, Keith Saunders, Roger Castells-Graells, Inmaculada Ferriol, Mattia Santoni, John F. C. Steele, Neil A. Ranson, Linda Avesani, Juan Jose Lopez-Moya and George P. 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FIJBBK+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 376 0 FIJBBM+PalatinoLinotype TrueType WinAnsi yes yes no 377 0 FIJBBO+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 378 0 FIJJAP+Arial CID TrueType Identity-H yes yes yes 379 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-03-08 02:36:19 CET RepresentationInformation: Vir_a2021v13n5p885iENG.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-03-04 08:39:22 CET Size: 1937171 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: きちぴづひどちぬびぜ〴ぜ〰ちのつぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぃぬはのどのでぜ〴ぜ〰はてぜ〴ぜ〰ぃはのびぴひふっぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ごひちのびどづのぴぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぎどっはぴどちのちぜ〴ぜ〰ぢづのぴとちねどちのちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Children: Item: Title: ぃぬはのどのでぜ〴ぜ〰はてぜ〴ぜ〰ぃはのびぴひふっぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰ごひちのびどづのぴぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぎどっはぴどちのちぜ〴ぜ〰ぢづのぴとちねどちのちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ごひちのびどづのぴぜ〴ぜ〰ぅへばひづびびどはのぜ〴ぜ〰どのぜ〴ぜ〰ぎどっはぴどちのちぜ〴ぜ〰ぢづのぴとちねどちのちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ぐひはぴづどのぜ〴ぜ〰ぅへぴひちっぴどはのぜ〴ぜ〰ちのつぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ぐふひどてどっちぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㘩‾㸊⡜㌷㙜㌷㝜〰ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ぎはひぴとづひのぜ〴ぜ〰あぬはぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㜩‾㸊⡜㌷㙜㌷㝜〰ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ごひちのびねどびびどはのぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴ぜ〰ぜ〵ぜ〰ごぅきぜ〵ㅜ〰ぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㠩‾㸊⡜㌷㙜㌷㝜〰ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Item: Title: ぃひべはぜ〴ぜ〰ぅぬづっぴひはのぜ〴ぜ〰きどっひはびっはばべぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸱ Children: Item: Title: ぉねちでづぜ〴ぜ〰ぐひはっづびびどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獵扳散瑩潮⸲⸸⸲ Item: Title: きはつづぬぜ〴ぜ〰あふどぬつどのでぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐはぴづへぶどひふびぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぁひづぜ〴ぜ〰ぅててどっどづのぴぬべぜ〴ぜ〰ぅへばひづびびづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐはぴづへぶどひふび〭あちびづつぜ〴ぜ〰ばぅててぜ〴ぜ〰ざづっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ざがぐびぜ〴ぜ〰てひはねぜ〴ぜ〰くぴとづひぜ〴ぜ〰うちねどぬどづびぜ〴ぜ〰はてぜ〴ぜ〰えづぬどっちぬぜ〴ぜ〰ざどひふびぜ〴ぜ〰ぃちのぜ〴ぜ〰あづぜ〴ぜ〰ぐひはつふっづつぜ〴ぜ〰ぴはぜ〴ぜ〰えどでとぜ〴ぜ〰がづぶづぬぜ〴ぜ〰さびどのでぜ〴ぜ〰ぴとづぜ〴ぜ〰ばぅててぜ〴ぜ〰ざづっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ちのつぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぅのっちばびどつちぴづぜ〴ぜ〰げぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ごとづぜ〴ぜ〰こぴひふっぴふひづぜ〴ぜ〰はてぜ〴ぜ〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰げづびづねぢぬづびぜ〴ぜ〰ぁふぴとづのぴどっぜ〴ぜ〰ぐはぴづへぶどひふびぜ〴ぜ〰ざどひどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: ぐはぴづへぶどひふびぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぁひづぜ〴ぜ〰ぅててどっどづのぴぬべぜ〴ぜ〰ぅへばひづびびづつぜ〴ぜ〰ぢべぜ〴ぜ〰ぴとづぜ〴ぜ〰ぐはぴづへぶどひふび〭あちびづつぜ〴ぜ〰ばぅててぜ〴ぜ〰ざづっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ざがぐびぜ〴ぜ〰てひはねぜ〴ぜ〰くぴとづひぜ〴ぜ〰うちねどぬどづびぜ〴ぜ〰はてぜ〴ぜ〰えづぬどっちぬぜ〴ぜ〰ざどひふびぜ〴ぜ〰ぃちのぜ〴ぜ〰あづぜ〴ぜ〰ぐひはつふっづつぜ〴ぜ〰ぴはぜ〴ぜ〰えどでとぜ〴ぜ〰がづぶづぬぜ〴ぜ〰さびどのでぜ〴ぜ〰ぴとづぜ〴ぜ〰ばぅててぜ〴ぜ〰ざづっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ちのつぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぅのっちばびどつちぴづぜ〴ぜ〰げぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ごとづぜ〴ぜ〰こぴひふっぴふひづぜ〴ぜ〰はてぜ〴ぜ〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰げづびづねぢぬづびぜ〴ぜ〰ぁふぴとづのぴどっぜ〴ぜ〰ぐはぴづへぶどひふびぜ〴ぜ〰ざどひどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ざがぐびぜ〴ぜ〰てひはねぜ〴ぜ〰くぴとづひぜ〴ぜ〰うちねどぬどづびぜ〴ぜ〰はてぜ〴ぜ〰えづぬどっちぬぜ〴ぜ〰ざどひふびぜ〴ぜ〰ぃちのぜ〴ぜ〰あづぜ〴ぜ〰ぐひはつふっづつぜ〴ぜ〰ぴはぜ〴ぜ〰えどでとぜ〴ぜ〰がづぶづぬぜ〴ぜ〰さびどのでぜ〴ぜ〰ぴとづぜ〴ぜ〰ばぅててぜ〴ぜ〰ざづっぴはひぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ちのつぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぅのっちばびどつちぴづぜ〴ぜ〰げぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ごとづぜ〴ぜ〰こぴひふっぴふひづぜ〴ぜ〰はてぜ〴ぜ〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰げづびづねぢぬづびぜ〴ぜ〰ぁふぴとづのぴどっぜ〴ぜ〰ぐはぴづへぶどひふびぜ〴ぜ〰ざどひどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ちのつぜ〴ぜ〰ごきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰ぅのっちばびどつちぴづぜ〴ぜ〰げぎぁぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㐩‾㸊⡜㌷㙜㌷㝜〰ごとづぜ〴ぜ〰こぴひふっぴふひづぜ〴ぜ〰はてぜ〴ぜ〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰げづびづねぢぬづびぜ〴ぜ〰ぁふぴとづのぴどっぜ〴ぜ〰ぐはぴづへぶどひふびぜ〴ぜ〰ざどひどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ごとづぜ〴ぜ〰こぴひふっぴふひづぜ〴ぜ〰はてぜ〴ぜ〰ばぅてて〭ぐひはつふっづつぜ〴ぜ〰ぁぬぴきざぜ〴ぜ〰ざがぐびぜ〴ぜ〰げづびづねぢぬづびぜ〴ぜ〰ぁふぴとづのぴどっぜ〴ぜ〰ぐはぴづへぶどひふびぜ〴ぜ〰ざどひどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: IJVWXD+SFTT0900 FontSubset: true FirstChar: 41 LastChar: 41 FontDescriptor: FontName: IJVWXD+SFTT0900 Flags: Symbolic FontBBox: -210, -359, 1376, 844 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HNZZIM+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: HNZZIM+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XIXXJP+URWPalladioL-Bold FontSubset: true FirstChar: 3 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