d73ff69b56083c9639fb35aea8962599 pathogens-a2021v10n8p1016.pdf 4c710fe93a03b81293ee47bf982e073e6abe4c34 pathogens-a2021v10n8p1016.pdf 1e91830887f985735e98dae860eb4f660e8a729cecf51001b55a518f1396d9a8 pathogens-a2021v10n8p1016.pdf Title: Genotyping of Porcine Circovirus 2 (PCV-2) in Vaccinated Pigs Suffering from PCV-2-Systemic Disease between 2009 and 2020 in Spain Subject: Vaccination against porcine circovirus 2 (PCV-2) is a common practice all over the world. Vaccines can prevent PCV-2-systemic disease (PCV-2-SD) outbreaks but not PCV-2 infection, which can be detectable in a percentage of vaccinated animals. Occasionally, PCV-2-SD is diagnosed in vaccinated farms. The objective of this study was to genotype the PCV-2 strains detected in vaccinated animals diagnosed with PCV-2-SD. Additionally, the evolution of the frequency of PCV-2 genotype detection at Spanish, European, and world levels was assessed. Fifty cases diagnosed as PCV-2-SD between 2009 and 2020 were included in this study. PCV-2 genotype was determined by sequencing the Cap gene region. Among them, only PCV-2b (23/50, 46%) and PCV-2d (27/50, 54%) genotypes were detected. Although the frequency of detection of these two genotypes was similar, their temporal distribution was different. Whereas most PCV-2b sequences (17/23, 74%) were detected between 2009 and 2012, PCV-2d sequences were obtained from 2013 to 2020. Indeed, a predominance of the PCV-2d genotype was observed from 2013 onwards, a trend also noticed at European and world levels. The results suggest that detection of particular genotypes in vaccinated animals probably reflects the general prevalence of the genotypes over time rather than genotype-specific vaccine-immunity escaping. Keywords: porcine circovirus 2; genotypes; PCV-2-SD; vaccination Author: Marina Sibila, Caterina Rocco, Giovanni Franzo, Eva Huerta, Mariano Domingo, José Ignacio Núñez and Joaquim Segalés Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Aug 12 09:51:16 2021 CEST ModDate: Thu Aug 12 09:54:42 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2991212 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MQFYIK+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 XYABOM+VnURWPalladioL Type 1 Custom yes yes no 15 0 NQWYET+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 OODWXZ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 HYENPM+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 37 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 59 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 63 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 67 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 84 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 90 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 96 0 FNTSBS+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 110 0 FNTSBS+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 116 0 FNTSBS+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 122 0 ECTHDD+VnURWPalladioL-Italic Type 1 Custom yes yes no 145 0 DKZMYW+CMSY10 Type 1 Builtin yes yes no 149 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 153 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-03-17 02:31:21 CET RepresentationInformation: pathogens-a2021v10n8p1016.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2022-03-10 12:12:52 CET Size: 2991212 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 285 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: PCV-2 Phylogenetic Analyses and Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: PCV-2 Genotype Frequencies at the World, European, and Spanish Levels Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Sample Selection Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Deparaffination and DNA Extraction Protocol Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: PCV-2 CAP Gene Amplification and Sequencing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: PCV-2 ORF2 Phylogenetic Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Phylogenetic Analyses of PCV-2 Sequences at World, European, and Spanish Levels Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Info: Title: Genotyping of Porcine Circovirus 2 (PCV-2) in Vaccinated Pigs Suffering from PCV-2-Systemic Disease between 2009 and 2020 in Spain Author: Marina Sibila, Caterina Rocco, Giovanni Franzo, Eva Huerta, Mariano Domingo, José Ignacio Núñez and Joaquim Segalés Subject: Vaccination against porcine circovirus 2 (PCV-2) is a common practice all over the world. Vaccines can prevent PCV-2-systemic disease (PCV-2-SD) outbreaks but not PCV-2 infection, which can be detectable in a percentage of vaccinated animals. Occasionally, PCV-2-SD is diagnosed in vaccinated farms. The objective of this study was to genotype the PCV-2 strains detected in vaccinated animals diagnosed with PCV-2-SD. Additionally, the evolution of the frequency of PCV-2 genotype detection at Spanish, European, and world levels was assessed. Fifty cases diagnosed as PCV-2-SD between 2009 and 2020 were included in this study. PCV-2 genotype was determined by sequencing the Cap gene region. Among them, only PCV-2b (23/50, 46%) and PCV-2d (27/50, 54%) genotypes were detected. Although the frequency of detection of these two genotypes was similar, their temporal distribution was different. Whereas most PCV-2b sequences (17/23, 74%) were detected between 2009 and 2012, PCV-2d sequences were obtained from 2013 to 2020. Indeed, a predominance of the PCV-2d genotype was observed from 2013 onwards, a trend also noticed at European and world levels. The results suggest that detection of particular genotypes in vaccinated animals probably reflects the general prevalence of the genotypes over time rather than genotype-specific vaccine-immunity escaping. Keywords: porcine circovirus 2; genotypes; PCV-2-SD; vaccination Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Aug 12 09:51:16 CEST 2021 ModDate: Thu Aug 12 09:54:42 CEST 2021 ID: 0xe469ad94aff86ce36c75888a91bc89ff, 0xe469ad94aff86ce36c75888a91bc89ff Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ECTHDD+VnURWPalladioL-Italic FontSubset: true FirstChar: 160 LastChar: 225 FontDescriptor: FontName: ECTHDD+VnURWPalladioL-Italic Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: NQWYET+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: NQWYET+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: DKZMYW+CMSY10 FontSubset: true FirstChar: 2 LastChar: 48 FontDescriptor: FontName: DKZMYW+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: OODWXZ+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 241 FontDescriptor: FontName: OODWXZ+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: MQFYIK+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 243 FontDescriptor: FontName: MQFYIK+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: HYENPM+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: HYENPM+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XYABOM+VnURWPalladioL FontSubset: true FirstChar: 160 LastChar: 225 FontDescriptor: FontName: XYABOM+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 35, 759, 168, 795 Annotation: Subtype: Link Rect: 515, 759, 560, 795 Annotation: Subtype: Link Rect: 124, 641, 136, 654 Annotation: Subtype: Link Rect: 316, 641, 327, 654 Annotation: Subtype: Link Rect: 136, 628, 147, 642 Annotation: Subtype: Link Rect: 263, 628, 274, 642 Annotation: Subtype: Link Rect: 82, 346, 154, 355 Annotation: Subtype: Link Rect: 35, 334, 123, 343 Annotation: Subtype: Link Rect: 35, 162, 97, 184 Annotation: Subtype: Link Rect: 153, 89, 154, 98 Annotation: Subtype: Link Rect: 35, 77, 147, 86 Annotation: Subtype: Link Rect: 440, 126, 447, 134 ActionDest: 160 Annotation: Subtype: Link Rect: 387, 113, 394, 122 ActionDest: 160 Annotation: Subtype: Link Rect: 122, 17, 283, 28 Annotation: Subtype: Link Rect: 398, 17, 558, 28 Annotation: Subtype: Link Rect: 35, 446, 87, 465 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 334, 699, 341, 708 ActionDest: 160 Annotation: Subtype: Link Rect: 213, 674, 220, 683 ActionDest: 160 Annotation: Subtype: Link Rect: 223, 674, 230, 683 ActionDest: 160 Annotation: Subtype: Link Rect: 343, 661, 350, 670 ActionDest: 160 Annotation: Subtype: Link Rect: 364, 649, 371, 658 ActionDest: 160 Annotation: Subtype: Link Rect: 534, 624, 541, 633 ActionDest: 160 Annotation: Subtype: Link Rect: 544, 624, 556, 633 ActionDest: 160 Annotation: Subtype: Link Rect: 394, 586, 406, 595 ActionDest: 160 Annotation: Subtype: Link Rect: 409, 586, 421, 595 ActionDest: 160 Annotation: Subtype: Link Rect: 399, 574, 411, 582 ActionDest: 160 Annotation: Subtype: Link Rect: 283, 561, 295, 570 ActionDest: 160 Annotation: Subtype: Link Rect: 213, 536, 225, 545 ActionDest: 160 Annotation: Subtype: Link Rect: 225, 536, 237, 545 ActionDest: 160 Annotation: Subtype: Link Rect: 535, 536, 547, 545 ActionDest: 160 Annotation: Subtype: Link Rect: 270, 485, 282, 495 ActionDest: 160 Annotation: Subtype: Link Rect: 199, 344, 206, 356 ActionDest: 79 Annotation: Subtype: Link Rect: 380, 281, 387, 293 ActionDest: 79 Page: Sequence: 3 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 362, 679, 369, 691 ActionDest: 105 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 497, 556, 509, 565 ActionDest: 160 Annotation: Subtype: Link Rect: 544, 531, 556, 540 ActionDest: 160 Annotation: Subtype: Link Rect: 387, 493, 399, 502 ActionDest: 160 Annotation: Subtype: Link Rect: 476, 481, 488, 490 ActionDest: 160 Annotation: Subtype: Link Rect: 491, 456, 503, 465 ActionDest: 160 Annotation: Subtype: Link Rect: 504, 456, 516, 465 ActionDest: 160 Annotation: Subtype: Link Rect: 497, 406, 509, 414 ActionDest: 160 Annotation: Subtype: Link Rect: 510, 405, 521, 414 ActionDest: 160 Annotation: Subtype: Link Rect: 510, 393, 521, 402 ActionDest: 160 Annotation: Subtype: Link Rect: 522, 393, 534, 402 ActionDest: 160 Annotation: Subtype: Link Rect: 317, 330, 329, 339 ActionDest: 163 Annotation: Subtype: Link Rect: 502, 317, 514, 327 ActionDest: 163 Annotation: Subtype: Link Rect: 379, 280, 391, 289 ActionDest: 163 Annotation: Subtype: Link Rect: 214, 142, 226, 151 ActionDest: 160 Annotation: Subtype: Link Rect: 226, 142, 238, 151 ActionDest: 160 Annotation: Subtype: Link Rect: 518, 104, 530, 113 ActionDest: 163 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 195, 668, 202, 677 ActionDest: 160 Annotation: Subtype: Link Rect: 489, 605, 501, 614 ActionDest: 163 Annotation: Subtype: Link Rect: 442, 480, 454, 489 ActionDest: 163 Annotation: Subtype: Link Rect: 545, 316, 557, 325 ActionDest: 163 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 253, 720, 451, 732 Annotation: Subtype: Link Rect: 322, 697, 334, 706 ActionDest: 163 Annotation: Subtype: Link Rect: 287, 685, 299, 694 ActionDest: 163 Annotation: Subtype: Link Rect: 287, 669, 380, 681 Annotation: Subtype: Link Rect: 169, 632, 349, 644 Annotation: Subtype: Link Rect: 490, 408, 497, 417 ActionDest: 160 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 86, 626, 123, 636 Annotation: Subtype: Link Rect: 438, 602, 476, 613 Annotation: Subtype: Link Rect: 482, 602, 518, 613 Annotation: Subtype: Link Rect: 476, 591, 513, 602 Annotation: Subtype: Link Rect: 520, 591, 556, 602 Annotation: Subtype: Link Rect: 433, 545, 471, 556 Annotation: Subtype: Link Rect: 187, 510, 224, 521 Annotation: Subtype: Link Rect: 419, 487, 456, 498 Annotation: Subtype: Link Rect: 462, 487, 498, 498 Annotation: Subtype: Link Rect: 258, 418, 296, 429 Annotation: Subtype: Link Rect: 344, 395, 381, 406 Annotation: Subtype: Link Rect: 518, 372, 555, 383 Annotation: Subtype: Link Rect: 479, 349, 515, 360 Annotation: Subtype: Link Rect: 521, 349, 557, 360 Annotation: Subtype: Link Rect: 329, 326, 366, 337 Annotation: Subtype: Link Rect: 59, 304, 97, 314 Annotation: Subtype: Link Rect: 103, 304, 139, 314 Annotation: Subtype: Link Rect: 220, 281, 257, 291 Annotation: Subtype: Link Rect: 264, 281, 300, 291 Annotation: Subtype: Link Rect: 393, 257, 430, 268 Annotation: Subtype: Link Rect: 437, 257, 473, 268 Annotation: Subtype: Link Rect: 104, 200, 141, 210 Annotation: Subtype: Link Rect: 148, 200, 184, 210 Annotation: Subtype: Link Rect: 277, 177, 315, 187 Annotation: Subtype: Link Rect: 386, 153, 423, 164 Annotation: Subtype: Link Rect: 458, 130, 496, 141 Annotation: Subtype: Link Rect: 502, 130, 538, 141 Annotation: Subtype: Link Rect: 95, 108, 133, 118 Annotation: Subtype: Link Rect: 191, 73, 228, 84 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 424, 721, 461, 732 Annotation: Subtype: Link Rect: 468, 721, 504, 732 Annotation: Subtype: Link Rect: 353, 698, 390, 709 Annotation: Subtype: Link Rect: 153, 675, 190, 686 Annotation: Subtype: Link Rect: 196, 675, 232, 686 Annotation: Subtype: Link Rect: 263, 652, 300, 663 Annotation: Subtype: Link Rect: 307, 652, 343, 663 Annotation: Subtype: Link Rect: 391, 583, 428, 594 Annotation: Subtype: Link Rect: 59, 549, 97, 559 Annotation: Subtype: Link Rect: 225, 525, 263, 536 Annotation: Subtype: Link Rect: 269, 525, 305, 536 Annotation: Subtype: Link Rect: 245, 503, 282, 513 Annotation: Subtype: Link Rect: 288, 503, 324, 513 Checksum: 31744ffd Type: CRC32 Checksum: d73ff69b56083c9639fb35aea8962599 Type: MD5 Checksum: 4c710fe93a03b81293ee47bf982e073e6abe4c34 Type: SHA-1