e3ac8529cb26e1cdea9db22fafd0cb4e catalysts_a2021v11n10p1167.pdf 66ca398205657b70d1817ce370bd9af0da5adc3a catalysts_a2021v11n10p1167.pdf c51b2817d0c56c0486b42c380d5ff667cda7e7c7d9b6448eadf26b09e24ff7d9 catalysts_a2021v11n10p1167.pdf Title: Catalyst Replacement Policy on Multienzymatic Systems: Theoretical Study in the One-Pot Sequential Batch Production of Lactofructose Syrup Subject: One-pot systems are an interesting proposal to carry out multi-enzymatic reactions, though this strategy implies establishing an optimal balance between the activity and operability of the involved enzymes. This is crucial for enzymes with marked differences in their operational stability, such as one-pot production of lactofructose syrup from cheese whey permeate, which involves two enzymes—-galactosidase (-gal) and glucose isomerase (GI). The aim of this work was to study the behavior of one-pot sequential batch production of lactofructose syrup considering both enzymes immobilized individually, in order to evaluate and design a strategy of replacement of the catalysts according to their stabilities. To this end, the modelling and simulation of the process was carried out, considering simultaneously the kinetics of both reactions and the kinetics of inactivation of both enzymes. For the latter, it was also considered the modulating effect that sugars present in the medium may have on the stability of -gal, which is the less stable enzyme. At the simulated reaction conditions of 40 C, pH 7, and 0.46 (IUGI/IU-gal), the results showed that considering the stability of -gal under non-reactive conditions, meaning in absence of the effect of modulation, it is necessary to carry out four replacements of -gal for each cycle of use of GI. On the other hand, when considering the modulation caused by the sugars on the -gal stability, the productivity increases up to 23% in the case of the highest modulation factor studied ( = 0.8). This work shows the feasibility of conducting a one-pot operation with immobilized enzymes of quite different operational stability, and that a proper strategy of biocatalyst replacement increases the productivity of the process. Keywords: multi-enzymatic reactions; one-pot; catalyst replacement policy; modulation factor; sequential batch; lactofructose syrup; -galactosidase; glucose isomerase Author: Pablo Silva, Vanessa Arancibia, Daniela Cid, Oscar Romero, Andrés Illanes and Lorena Wilson Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Oct 12 06:16:04 2021 CEST ModDate: Tue Oct 12 06:29:44 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2242160 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FQWRUN+VnURWPalladioL Type 1 Custom yes yes no 10 0 DUYMIB+EURM10 Type 1 Builtin yes yes no 15 0 HHFREY+EURM7 Type 1 Builtin yes yes no 19 0 FCTACA+URWPalladioL-Ital Type 1 Custom yes yes no 23 0 YLSWKD+URWPalladioL-Bold Type 1 Custom yes yes no 28 0 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TrueType WinAnsi yes yes no 228 0 HBGDOM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 231 0 CRPVJA+CMEX10 Type 1 Builtin yes yes no 238 0 GIGFZE+CMR10 Type 1 Builtin yes yes no 242 0 HVSBKL+PazoMath Type 1 Builtin yes yes no 246 0 ZYYJXI+EURB10 Type 1 Builtin yes yes no 256 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:26:26 CEST RepresentationInformation: catalysts_a2021v11n10p1167.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 11:58:52 CEST Size: 2242160 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 446 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results and Discussions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Selection of Operating Conditions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Effect of Modulation of -gal Stability on the Production of Lactofructose Syrup in One-Pot Sequential Batch Operation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: Materials Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Modeling Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Enzyme Kinetics Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Enzyme Inactivation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Mathematical Model for the One-Pot Production of Lactofructose Syrup in Sequential Batch Operation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Reactor Operation Conditions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Metrics Used in Reactor Operations Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Info: Title: Catalyst Replacement Policy on Multienzymatic Systems: Theoretical Study in the One-Pot Sequential Batch Production of Lactofructose Syrup Author: Pablo Silva, Vanessa Arancibia, Daniela Cid, Oscar Romero, Andrés Illanes and Lorena Wilson Subject: One-pot systems are an interesting proposal to carry out multi-enzymatic reactions, though this strategy implies establishing an optimal balance between the activity and operability of the involved enzymes. This is crucial for enzymes with marked differences in their operational stability, such as one-pot production of lactofructose syrup from cheese whey permeate, which involves two enzymes -galactosidase (-gal) and glucose isomerase (GI). The aim of this work was to study the behavior of one-pot sequential batch production of lactofructose syrup considering both enzymes immobilized individually, in order to evaluate and design a strategy of replacement of the catalysts according to their stabilities. To this end, the modelling and simulation of the process was carried out, considering simultaneously the kinetics of both reactions and the kinetics of inactivation of both enzymes. For the latter, it was also considered the modulating effect that sugars present in the medium may have on the stability of -gal, which is the less stable enzyme. At the simulated reaction conditions of 40 C, pH 7, and 0.46 (IUGI/IU-gal), the results showed that considering the stability of -gal under non-reactive conditions, meaning in absence of the effect of modulation, it is necessary to carry out four replacements of -gal for each cycle of use of GI. On the other hand, when considering the modulation caused by the sugars on the -gal stability, the productivity increases up to 23% in the case of the highest modulation factor studied ( = 0.8). This work shows the feasibility of conducting a one-pot operation with immobilized enzymes of quite different operational stability, and that a proper strategy of biocatalyst replacement increases the productivity of the process. 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