956ec70ebbbdc1324cbecb9fb9550749 chemosensors_a2021m12v9n12p330.pdf edd9a90e0a44be932498471235d4f07f0d012f8d chemosensors_a2021m12v9n12p330.pdf 4cf5ab6882e64a979ce68881f483e61f738c7bfef880fc5ae391357e855df7ad chemosensors_a2021m12v9n12p330.pdf Title: Current Trends in SPR Biosensing of SARS-CoV-2 Entry Inhibitors Subject: The emerging risk of viral diseases has triggered the search for preventive and therapeutic agents. Since the beginning of the COVID-19 pandemic, greater efforts have been devoted to investigating virus entry mechanisms into host cells. The feasibility of plasmonic sensing technologies for screening interactions of small molecules in real time, while providing the pharmacokinetic drug profiling of potential antiviral compounds, offers an advantageous approach over other biophysical methods. This review summarizes recent advancements in the drug discovery process of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) inhibitors using Surface Plasmon Resonance (SPR) biosensors. A variety of SPR assay formats are discussed according to the binding kinetics and drug efficacies of both natural products and repurposed drugs. Special attention has been given to the targeting of antiviral agents that block the receptor binding domain of the spike protein (RBD-S) and the main protease (3CLpro) of SARS-CoV-2. The functionality of plasmonic biosensors for high-throughput screening of entry virus inhibitors was also reviewed taking into account experimental parameters (binding affinities, selectivity, stability), potential limitations and future applications. Keywords: plasmonic; SPR biosensor; viral diagnostics; virus entry inhibitors; drug screening; antiviral agents; SARS-CoV-2; COVID-19 Author: Elba Mauriz and Laura M. Lechuga Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Nov 25 10:45:06 2021 CET ModDate: Thu Nov 25 11:03:58 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 25 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4610375 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JWJJUF+VnURWPalladioL Type 1 Custom yes yes no 10 0 QHROGP+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 KQEKJR+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 ITIEDQ+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 QOQMCA+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 32 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 54 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 58 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 62 0 PGPLKH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: SPR Analysis of SARS-CoV-2 Entry Inhibitors Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: SPR Strategies for Studying the Blocking of ACE2 Receptors Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Children: Item: Title: SPR Biosensor as Primary Screening Method Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Kinetic Analysis of Natural Products Obtained from Plants Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: SPR Role in Drug Repurposing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: SPR Biosensors as Diagnostic Tools to Detect Neutralizing Antibodies Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: SPR Strategies for the Development of SARS-CoV-2 Main Protease Inhibitors Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: SPR Analysis of Mpro Inhibitors from Plant Origin Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Discovery of Mpro Inhibitors via Drug Repurposing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Conclusions and Future Perspectives Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Info: Title: Current Trends in SPR Biosensing of SARS-CoV-2 Entry Inhibitors Author: Elba Mauriz and Laura M. Lechuga Subject: The emerging risk of viral diseases has triggered the search for preventive and therapeutic agents. Since the beginning of the COVID-19 pandemic, greater efforts have been devoted to investigating virus entry mechanisms into host cells. The feasibility of plasmonic sensing technologies for screening interactions of small molecules in real time, while providing the pharmacokinetic drug profiling of potential antiviral compounds, offers an advantageous approach over other biophysical methods. This review summarizes recent advancements in the drug discovery process of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) inhibitors using Surface Plasmon Resonance (SPR) biosensors. A variety of SPR assay formats are discussed according to the binding kinetics and drug efficacies of both natural products and repurposed drugs. Special attention has been given to the targeting of antiviral agents that block the receptor binding domain of the spike protein (RBD-S) and the main protease (3CLpro) of SARS-CoV-2. The functionality of plasmonic biosensors for high-throughput screening of entry virus inhibitors was also reviewed taking into account experimental parameters (binding affinities, selectivity, stability), potential limitations and future applications. 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