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Title:          Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells
Subject:        Nano Today, 36 (2021) 101056. doi:10.1016/j.nantod.2020.101056
Keywords:       HIV; Bispecific nanoparticles; NK cells; Polarization
Author:         Antonio Astorga-Gamaza
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      Title: Antibody cooperative adsorption onto AuNPs and its exploitation to force natural killer cells to kill HIV-infected T cells
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        Title: Introduction
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        Title: Results
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          Title: Generation of functionalized bispecific nanoparticles
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          Title: Polarization assessment after following the cooperative adsorption conjugation protocol
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          Title: BiAb-AuNPs promote specific cell-to-cell contact between CD4-expressing HIV and NK cells
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          Title: pBiAb-AuNPs induce a potent cytotoxic response against primary CD4 T cells from HIV-infected patients and against ex vivo human infected tonsillar explants
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          Title: pBiAb-AuNPs strongly enhance the NK-mediated killing of latent HIV-infected cells after viral reactivation
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        Title: Discussion
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        Title: Materials and methods
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          Title: Ethics statement
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          Title: Cells, antibodies and reagents
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          Title: Synthesis of gold nanoparticles and conjugation with antibodies
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            Title: Nanoparticle synthesis
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            Title: Nanoparticle conjugation
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          Title: Physicochemical characterization of BiAb-AuNPs
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            Title: TEM - SEM analysis
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            Title: UV-Visible absorption spectroscopy
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            Title: DLS and Zeta potential measurements
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          Title: Bioconjugation to Nivolumab
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          Title: Distribution of antibodies on cooperatively-conjugated AuNPs by TEM
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          Title: Thermogravimetric assay
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        Title: Declaration of competing interest
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  Checksum: c56f545f
   Type: CRC32
  Checksum: e55140900e648f12eee61ebfe2e3f9fc
   Type: MD5
  Checksum: 31ffcf9bb19ece68381aac5e61a93031d4e539d6
   Type: SHA-1