87c6d530e961f3f5142198424dab9e44 VIRS_a2021v13p2404.pdf a2eedf9dbea4777e0e9d796ff7c77ed8d2ddeef6 VIRS_a2021v13p2404.pdf bf528518b4a82e09930ca8d5bedb21b9a94e35c14cde7a59126ca5ab9ecd2b02 VIRS_a2021v13p2404.pdf Title: Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 Subject: The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework. Keywords: West Nile virus; Usutu virus; Bagaza virus; tick-borne encephalitis virus; flaviviruses; Spain Author: Sebastian Napp, Francisco Llorente, Cécile Beck, Eduard Jose-Cunilleras, Mercè Soler, Lola Pailler-García, Rayane Amaral, Pilar Aguilera-Sepúlveda, Maria Pifarré, Rafael Molina-López, Elena Obón, Olga Nicolás, Sylvie Lecollinet, Miguel Ángel Jiménez-Clavero and Núria Busquets Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Dec 1 08:53:05 2021 CET ModDate: Wed Dec 1 09:12:24 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 852898 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EHMQIV+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 GKFIUK+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 RPACBK+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 WHLIZK+VnURWPalladioL-Bold Type 1 Custom yes yes no 23 0 BTJSEZ+VnURWPalladioL Type 1 Custom yes yes no 28 0 HOWKDX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 ZYGMNY+CMSY10 Type 1 Builtin yes yes no 81 0 ALFNIN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 98 0 ALFNIP+PalatinoLinotype TrueType WinAnsi yes yes no 101 0 ALFNJB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 104 0 WBVKDJ+URWPalladioL-BoldItal Type 1 Custom yes yes no 114 0 ALFNIN+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 125 0 ALFNIP+PalatinoLinotype TrueType WinAnsi yes yes no 128 0 ALFNJB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 131 0 ALGANA+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 134 0 SUEOXN+VnURWPalladioL-Italic Type 1 Custom yes yes no 150 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-04-26 02:32:37 CEST RepresentationInformation: VIRS_a2021v13p2404.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-04-25 11:34:12 CEST Size: 852898 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 356 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Laboratory Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Children: Item: Title: Birds Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Horses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Spatial Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Flaviviruses in Birds Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Flaviviruses in Horses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Spatial Analyses Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Info: Title: Widespread Circulation of Flaviviruses in Horses and Birds in Northeastern Spain (Catalonia) between 2010 and 2019 Author: Sebastian Napp, Francisco Llorente, Cécile Beck, Eduard Jose-Cunilleras, Mercè Soler, Lola Pailler-García, Rayane Amaral, Pilar Aguilera-Sepúlveda, Maria Pifarré, Rafael Molina-López, Elena Obón, Olga Nicolás, Sylvie Lecollinet, Miguel Ángel Jiménez-Clavero and Núria Busquets Subject: The surveillance for West Nile virus (WNV) in Catalonia (northeastern Spain) has consistently detected flaviviruses not identified as WNV. With the aim of characterizing the flaviviruses circulating in Catalonia, serum samples from birds and horses collected between 2010 and 2019 and positive by panflavivirus competition ELISA (cELISA) were analyzed by microneutralization test (MNT) against different flaviviruses. A third of the samples tested were inconclusive by MNT, highlighting the limitations of current diagnostic techniques. Our results evidenced the widespread circulation of flaviviruses, in particular WNV, but also Usutu virus (USUV), and suggest that chicken and horses could serve as sentinels for both viruses. In several regions, WNV and USUV overlapped, but no significant geographical aggregation was observed. Bagaza virus (BAGV) was not detected in birds, while positivity to tick-borne encephalitis virus (TBEV) was sporadically detected in horses although no endemic foci were observed. So far, no human infections by WNV, USUV, or TBEV have been reported in Catalonia. However, these zoonotic flaviviruses need to be kept under surveillance, ideally within a One Health framework. Keywords: West Nile virus; Usutu virus; Bagaza virus; tick-borne encephalitis virus; flaviviruses; Spain Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Dec 01 08:53:05 CET 2021 ModDate: Wed Dec 01 09:12:24 CET 2021 ID: 0xe3a9b5edc58df089f870df404513ce24, 0xe3a9b5edc58df089f870df404513ce24 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ZYGMNY+CMSY10 FontSubset: true FirstChar: 14 LastChar: 21 FontDescriptor: FontName: ZYGMNY+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: WBVKDJ+URWPalladioL-BoldItal FontSubset: true FirstChar: 112 LastChar: 112 FontDescriptor: FontName: WBVKDJ+URWPalladioL-BoldItal Flags: Symbolic FontBBox: -170, -300, 1073, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: RPACBK+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: RPACBK+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: SUEOXN+VnURWPalladioL-Italic FontSubset: true FirstChar: 160 LastChar: 226 FontDescriptor: FontName: SUEOXN+VnURWPalladioL-Italic Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WHLIZK+VnURWPalladioL-Bold FontSubset: true FirstChar: 129 LastChar: 243 FontDescriptor: FontName: WHLIZK+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: EHMQIV+URWPalladioL-Ital FontSubset: true FirstChar: 39 LastChar: 121 FontDescriptor: FontName: EHMQIV+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: BTJSEZ+VnURWPalladioL FontSubset: true FirstChar: 128 LastChar: 243 FontDescriptor: FontName: BTJSEZ+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: GKFIUK+URWPalladioL-Bold FontSubset: true FirstChar: 2 LastChar: 122 FontDescriptor: FontName: GKFIUK+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 35, 759, 147, 795 Annotation: Subtype: Link Rect: 515, 759, 560, 795 Annotation: Subtype: Link Rect: 280, 659, 291, 673 Annotation: Subtype: Link Rect: 413, 659, 424, 673 Annotation: Subtype: Link Rect: 95, 634, 106, 648 Annotation: Subtype: Link Rect: 274, 634, 285, 648 Annotation: Subtype: Link Rect: 447, 634, 458, 648 Annotation: Subtype: Link Rect: 139, 622, 150, 635 Annotation: Subtype: Link Rect: 105, 358, 154, 367 Annotation: Subtype: Link Rect: 35, 346, 122, 355 Annotation: Subtype: Link Rect: 35, 162, 97, 184 Annotation: Subtype: Link Rect: 153, 89, 154, 98 Annotation: Subtype: Link Rect: 35, 77, 147, 86 Annotation: Subtype: Link Rect: 112, 17, 241, 28 Annotation: Subtype: Link Rect: 409, 17, 558, 28 Annotation: Subtype: Link Rect: 35, 470, 87, 489 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 398, 686, 405, 695 ActionDest: 147 Annotation: Subtype: Link Rect: 350, 649, 357, 658 ActionDest: 147 Annotation: Subtype: Link Rect: 199, 611, 206, 620 ActionDest: 147 Annotation: Subtype: Link Rect: 327, 561, 334, 570 ActionDest: 147 Annotation: Subtype: Link Rect: 251, 548, 258, 557 ActionDest: 147 Annotation: Subtype: Link Rect: 294, 523, 301, 532 ActionDest: 147 Annotation: Subtype: Link Rect: 534, 511, 541, 520 ActionDest: 147 Annotation: Subtype: Link Rect: 474, 485, 481, 495 ActionDest: 147 Annotation: Subtype: Link Rect: 482, 460, 489, 469 ActionDest: 147 Annotation: Subtype: Link Rect: 393, 435, 405, 444 ActionDest: 147 Annotation: Subtype: Link Rect: 441, 410, 453, 419 ActionDest: 147 Annotation: Subtype: Link Rect: 431, 398, 443, 407 ActionDest: 147 Annotation: Subtype: Link Rect: 291, 372, 303, 382 ActionDest: 147 Annotation: Subtype: Link Rect: 544, 360, 556, 369 ActionDest: 147 Annotation: Subtype: Link Rect: 204, 335, 216, 344 ActionDest: 147 Annotation: Subtype: Link Rect: 274, 322, 286, 331 ActionDest: 147 Annotation: Subtype: Link Rect: 318, 285, 330, 294 ActionDest: 147 Annotation: Subtype: Link Rect: 331, 285, 343, 294 ActionDest: 155 Annotation: Subtype: Link Rect: 352, 259, 364, 269 ActionDest: 155 Annotation: Subtype: Link Rect: 367, 222, 379, 231 ActionDest: 155 Annotation: Subtype: Link Rect: 511, 184, 523, 193 ActionDest: 155 Annotation: Subtype: Link Rect: 233, 159, 245, 168 ActionDest: 155 Annotation: Subtype: Link Rect: 351, 147, 358, 156 ActionDest: 147 Annotation: Subtype: Link Rect: 334, 134, 346, 143 ActionDest: 155 Annotation: Subtype: Link Rect: 544, 109, 556, 118 ActionDest: 155 Annotation: Subtype: Link Rect: 190, 71, 202, 80 ActionDest: 155 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 403, 735, 415, 744 ActionDest: 155 Annotation: Subtype: Link Rect: 206, 685, 218, 694 ActionDest: 155 Annotation: Subtype: Link Rect: 339, 672, 351, 681 ActionDest: 155 Annotation: Subtype: Link Rect: 544, 622, 556, 631 ActionDest: 155 Annotation: Subtype: Link Rect: 507, 559, 519, 568 ActionDest: 155 Annotation: Subtype: Link Rect: 419, 534, 426, 543 ActionDest: 147 Annotation: Subtype: Link Rect: 321, 509, 333, 518 ActionDest: 155 Annotation: Subtype: Link Rect: 334, 509, 346, 518 ActionDest: 155 Annotation: Subtype: Link Rect: 498, 459, 510, 468 ActionDest: 155 Annotation: Subtype: Link Rect: 338, 433, 345, 443 ActionDest: 147 Annotation: Subtype: Link Rect: 346, 434, 358, 443 ActionDest: 155 Annotation: Subtype: Link Rect: 358, 433, 370, 443 ActionDest: 155 Annotation: Subtype: Link Rect: 280, 92, 292, 102 ActionDest: 155 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 473, 446, 485, 455 ActionDest: 155 Annotation: Subtype: Link Rect: 528, 282, 540, 291 ActionDest: 155 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 303, 679, 310, 691 ActionDest: 88 Annotation: Subtype: Link Rect: 203, 379, 210, 388 ActionDest: 108 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 203, 353, 210, 363 ActionDest: 108 Annotation: Subtype: Link Rect: 441, 171, 448, 183 ActionDest: 111 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 248, 499, 255, 511 ActionDest: 88 Annotation: Subtype: Link Rect: 274, 499, 281, 511 ActionDest: 111 Annotation: Subtype: Link Rect: 331, 486, 338, 498 ActionDest: 88 Annotation: Subtype: Link Rect: 358, 486, 365, 498 ActionDest: 111 Annotation: Subtype: Link Rect: 417, 449, 424, 461 ActionDest: 111 Annotation: Subtype: Link Rect: 257, 423, 264, 435 ActionDest: 111 Annotation: Subtype: Link Rect: 254, 222, 266, 231 ActionDest: 155 Annotation: Subtype: Link Rect: 396, 159, 403, 168 ActionDest: 147 Annotation: Subtype: Link Rect: 305, 84, 317, 93 ActionDest: 155 Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 203, 722, 215, 731 ActionDest: 147 Annotation: Subtype: Link Rect: 239, 710, 251, 719 ActionDest: 147 Annotation: Subtype: Link Rect: 470, 672, 482, 681 ActionDest: 155 Annotation: Subtype: Link Rect: 501, 634, 513, 643 ActionDest: 155 Annotation: Subtype: Link Rect: 514, 634, 526, 643 ActionDest: 155 Annotation: Subtype: Link Rect: 287, 622, 298, 631 ActionDest: 155 Annotation: Subtype: Link Rect: 289, 534, 301, 543 ActionDest: 155 Annotation: Subtype: Link Rect: 415, 434, 427, 443 ActionDest: 155 Annotation: Subtype: Link Rect: 427, 433, 439, 443 ActionDest: 155 Annotation: Subtype: Link Rect: 415, 408, 427, 417 ActionDest: 155 Annotation: Subtype: Link Rect: 400, 308, 412, 317 ActionDest: 155 Annotation: Subtype: Link Rect: 507, 270, 519, 279 ActionDest: 155 Annotation: Subtype: Link Rect: 241, 183, 252, 192 ActionDest: 155 Annotation: Subtype: Link Rect: 537, 157, 544, 166 ActionDest: 147 Annotation: Subtype: Link Rect: 544, 157, 555, 166 ActionDest: 155 Annotation: Subtype: Link Rect: 328, 69, 340, 79 ActionDest: 155 Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 341, 685, 353, 694 ActionDest: 158 Annotation: Subtype: Link Rect: 490, 634, 502, 643 ActionDest: 155 Annotation: Subtype: Link Rect: 434, 584, 441, 593 ActionDest: 147 Annotation: Subtype: Link Rect: 374, 534, 386, 543 ActionDest: 155 Annotation: Subtype: Link Rect: 299, 484, 311, 493 ActionDest: 158 Annotation: Subtype: Link Rect: 381, 421, 393, 430 ActionDest: 158 Annotation: Subtype: Link Rect: 397, 371, 409, 380 ActionDest: 158 Annotation: Subtype: Link Rect: 203, 320, 215, 330 ActionDest: 158 Annotation: Subtype: Link Rect: 296, 308, 308, 317 ActionDest: 158 Annotation: Subtype: Link Rect: 503, 308, 515, 317 ActionDest: 158 Annotation: Subtype: Link Rect: 251, 295, 263, 304 ActionDest: 158 Annotation: Subtype: Link Rect: 301, 233, 313, 242 ActionDest: 158 Annotation: Subtype: Link Rect: 503, 157, 515, 166 ActionDest: 158 Annotation: Subtype: Link Rect: 246, 107, 258, 116 ActionDest: 158 Annotation: Subtype: Link Rect: 198, 82, 210, 91 ActionDest: 158 Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 256, 672, 268, 681 ActionDest: 158 Annotation: Subtype: Link Rect: 236, 634, 248, 643 ActionDest: 158 Annotation: Subtype: Link Rect: 351, 546, 363, 556 ActionDest: 155 Annotation: Subtype: Link Rect: 280, 521, 292, 530 ActionDest: 155 Annotation: Subtype: Link Rect: 525, 496, 532, 505 ActionDest: 147 Annotation: Subtype: Link Rect: 424, 181, 560, 192 Annotation: Subtype: Link Rect: 166, 169, 258, 180 Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 429, 455, 467, 466 Annotation: Subtype: Link Rect: 269, 421, 306, 431 Annotation: Subtype: Link Rect: 437, 410, 475, 420 Annotation: Subtype: Link Rect: 481, 410, 517, 420 Annotation: Subtype: Link Rect: 332, 386, 369, 397 Annotation: Subtype: Link Rect: 376, 386, 412, 397 Annotation: Subtype: Link Rect: 59, 318, 97, 328 Annotation: Subtype: Link Rect: 103, 318, 139, 328 Annotation: Subtype: Link Rect: 454, 294, 492, 305 Annotation: Subtype: Link Rect: 59, 260, 97, 270 Annotation: Subtype: Link Rect: 103, 260, 139, 270 Annotation: Subtype: Link Rect: 242, 236, 280, 247 Annotation: Subtype: Link Rect: 480, 225, 517, 236 Annotation: Subtype: Link Rect: 59, 156, 97, 167 Annotation: Subtype: Link Rect: 103, 156, 139, 167 Annotation: Subtype: Link Rect: 108, 133, 146, 144 Annotation: Subtype: Link Rect: 345, 87, 382, 98 Annotation: Subtype: Link Rect: 436, 63, 474, 74 Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 238, 721, 276, 732 Annotation: Subtype: Link Rect: 212, 698, 250, 709 Annotation: Subtype: Link Rect: 256, 698, 292, 709 Annotation: Subtype: Link Rect: 454, 675, 491, 686 Annotation: Subtype: Link Rect: 322, 652, 360, 663 Annotation: Subtype: Link Rect: 59, 618, 97, 628 Annotation: Subtype: Link Rect: 103, 618, 139, 628 Annotation: Subtype: Link Rect: 95, 595, 132, 605 Annotation: Subtype: Link Rect: 139, 595, 175, 605 Annotation: Subtype: Link Rect: 520, 583, 557, 594 Annotation: Subtype: Link Rect: 315, 560, 560, 571 Annotation: Subtype: Link Rect: 56, 548, 134, 559 Annotation: Subtype: Link Rect: 140, 515, 178, 525 Annotation: Subtype: Link Rect: 451, 491, 489, 502 Annotation: Subtype: Link Rect: 495, 491, 531, 502 Annotation: Subtype: Link Rect: 392, 468, 430, 479 Annotation: Subtype: Link Rect: 217, 445, 254, 456 Annotation: Subtype: Link Rect: 109, 411, 146, 421 Annotation: Subtype: Link Rect: 95, 377, 132, 387 Annotation: Subtype: Link Rect: 234, 341, 272, 352 Annotation: Subtype: Link Rect: 219, 308, 257, 318 Annotation: Subtype: Link Rect: 487, 295, 525, 306 Annotation: Subtype: Link Rect: 234, 262, 271, 272 Annotation: Subtype: Link Rect: 155, 227, 193, 237 Annotation: Subtype: Link Rect: 199, 227, 235, 237 Annotation: Subtype: Link Rect: 270, 192, 308, 203 Annotation: Subtype: Link Rect: 314, 192, 350, 203 Annotation: Subtype: Link Rect: 517, 180, 555, 191 Annotation: Subtype: Link Rect: 294, 158, 331, 168 Annotation: Subtype: Link Rect: 337, 158, 373, 168 Annotation: Subtype: Link Rect: 300, 134, 337, 145 Annotation: Subtype: Link Rect: 268, 112, 306, 122 Annotation: Subtype: Link Rect: 312, 112, 348, 122 Annotation: Subtype: Link Rect: 226, 88, 264, 99 Page: Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 95, 710, 133, 720 Annotation: Subtype: Link Rect: 474, 698, 560, 709 Annotation: Subtype: Link Rect: 86, 653, 124, 663 Annotation: Subtype: Link Rect: 86, 629, 520, 640 Annotation: Subtype: Link Rect: 212, 583, 250, 594 Annotation: Subtype: Link Rect: 427, 560, 465, 571 Annotation: Subtype: Link Rect: 471, 560, 507, 571 Annotation: Subtype: Link Rect: 59, 526, 97, 536 Annotation: Subtype: Link Rect: 103, 526, 139, 536 Annotation: Subtype: Link Rect: 470, 502, 507, 513 Annotation: Subtype: Link Rect: 513, 502, 549, 513 Annotation: Subtype: Link Rect: 227, 479, 264, 490 Annotation: Subtype: Link Rect: 299, 399, 337, 410 Annotation: Subtype: Link Rect: 59, 377, 97, 387 Annotation: Subtype: Link Rect: 103, 377, 139, 387 Annotation: Subtype: Link Rect: 144, 342, 182, 352 Annotation: Subtype: Link Rect: 188, 342, 224, 352 Checksum: 6c224901 Type: CRC32 Checksum: 87c6d530e961f3f5142198424dab9e44 Type: MD5 Checksum: a2eedf9dbea4777e0e9d796ff7c77ed8d2ddeef6 Type: SHA-1