a3593fdb96143e56ff3f8b9a3625d78c 269855.pdf 031c154857df551a12bbe00ee130a51ee874a1a0 269855.pdf be4169d956a140032d7b279ecf3affb0e002f5aad47dddf2a12cf0c10cd59627 269855.pdf Title: Adenosine A2A Receptors Are Upregulated in Peripheral Blood Mononuclear Cells from Atrial Fibrillation Patients Subject: Atrial fibrillation (AF) is the most common form of cardiac arrhythmia seen in clinical practice. While some clinical parameters may predict the transition from paroxysmal to persistent AF, the molecular mechanisms behind the AF perpetuation are poorly understood. Thus, oxidative stress, calcium overload and inflammation, among others, are believed to be involved in AF-induced atrial remodelling. Interestingly, adenosine and its receptors have also been related to AF development and perpetuation. Here, we investigated the expression of adenosine A2A receptor (A2AR) both in right atrium biopsies and peripheral blood mononuclear cells (PBMCs) from non-dilated sinus rhythm (ndSR), dilated sinus rhythm (dSR) and AF patients. In addition, plasma adenosine content and adenosine deaminase (ADA) activity in these subjects were also determined. Our results revealed increased A2AR expression in the right atrium from AF patients, as previously described. Interestingly, increased levels of adenosine content and reduced ADA activity in plasma from AF patients were detected. An increase was observed when A2AR expression was assessed in PBMCs from AF subjects. Importantly, a positive correlation (p = 0.001) between A2AR expression in the right atrium and PBMCs was observed. Overall, these results highlight the importance of the A2AR in AF and suggest that the evaluation of this receptor in PBMCs may be potentially be useful in monitoring disease severity and the efficacy of pharmacological treatments in AF patients. Keywords: adenosine A2A receptor; atrial fibrillation; adenosine deaminase; peripheral blood mononuclear cells; adenosine Author: Héctor Godoy-Marín, Romain Duroux, Kenneth A. Jacobson, Concepció Soler, Hildegard Colino-Lage, Veronica Jiménez-Sábado, José Montiel, Leif Hove-Madsen and Francisco Ciruela Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Apr 8 07:34:34 2021 CEST ModDate: Fri Apr 9 05:02:58 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3041799 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 DUFXDP+VnURWPalladioL Type 1 Custom yes yes no 15 0 FPNCLJ+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 WYIMZH+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 DQFFVC+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 IRWHZX+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 36 0 CKUPDE+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 39 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 61 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 65 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 69 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 81 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 88 0 AHKEML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 99 0 AHKEMM+PalatinoLinotype CID TrueType Identity-H yes yes yes 102 0 AHKEMN+PalatinoLinotype TrueType WinAnsi yes yes no 108 0 AHKEMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 111 0 AHKJDG+TimesNewRoman CID TrueType Identity-H yes yes yes 114 0 AHKEML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 131 0 AHKEMM+PalatinoLinotype CID TrueType Identity-H yes yes yes 134 0 AHKEMN+PalatinoLinotype TrueType WinAnsi yes yes no 140 0 AHKEMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 143 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 150 0 AHKEML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 161 0 AHKEMM+PalatinoLinotype CID TrueType Identity-H yes yes yes 164 0 AHKEMN+PalatinoLinotype TrueType WinAnsi yes yes no 170 0 AHKEMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 173 0 AHKEML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 185 0 AHKEMM+PalatinoLinotype CID TrueType Identity-H yes yes yes 188 0 AHKEMN+PalatinoLinotype TrueType WinAnsi yes yes no 194 0 AHKEMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 197 0 AHKEML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 212 0 AHKEMM+PalatinoLinotype CID TrueType Identity-H yes yes yes 215 0 AHKEMN+PalatinoLinotype TrueType WinAnsi yes yes no 221 0 AHKEMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 224 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:13:35 CEST RepresentationInformation: 269855.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:04:46 CEST Size: 3041799 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 398 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Children: Item: Title: Reagents Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Human Samples (Subject Demographics) Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Membrane Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Gel Electrophoresis and Immunoblotting Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: RT-qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Sample Preparation and HPLC-MS/MS Determination of Ribonucleosides Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Adenosine Deaminase Activity Determination Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Cell Culture Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Flow Cytometry and Confocal Imaging Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Data and Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Info: Title: Adenosine A2A Receptors Are Upregulated in Peripheral Blood Mononuclear Cells from Atrial Fibrillation Patients Author: Héctor Godoy-Marín, Romain Duroux, Kenneth A. Jacobson, Concepció Soler, Hildegard Colino-Lage, Veronica Jiménez-Sábado, José Montiel, Leif Hove-Madsen and Francisco Ciruela Subject: Atrial fibrillation (AF) is the most common form of cardiac arrhythmia seen in clinical practice. While some clinical parameters may predict the transition from paroxysmal to persistent AF, the molecular mechanisms behind the AF perpetuation are poorly understood. Thus, oxidative stress, calcium overload and inflammation, among others, are believed to be involved in AF-induced atrial remodelling. Interestingly, adenosine and its receptors have also been related to AF development and perpetuation. Here, we investigated the expression of adenosine A2A receptor (A2AR) both in right atrium biopsies and peripheral blood mononuclear cells (PBMCs) from non-dilated sinus rhythm (ndSR), dilated sinus rhythm (dSR) and AF patients. In addition, plasma adenosine content and adenosine deaminase (ADA) activity in these subjects were also determined. Our results revealed increased A2AR expression in the right atrium from AF patients, as previously described. Interestingly, increased levels of adenosine content and reduced ADA activity in plasma from AF patients were detected. An increase was observed when A2AR expression was assessed in PBMCs from AF subjects. Importantly, a positive correlation (p = 0.001) between A2AR expression in the right atrium and PBMCs was observed. Overall, these results highlight the importance of the A2AR in AF and suggest that the evaluation of this receptor in PBMCs may be potentially be useful in monitoring disease severity and the efficacy of pharmacological treatments in AF patients. 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