b466aef752f58e9cdf8fda03d87c17b6 270616.pdf 3549a8e898ebfa1eba37fc9c19e768ac280be336 270616.pdf bf9304215e3b190f64f7cb0328da66ab0e67d711988ab310afa3d179616322ff 270616.pdf Title: Combining microfluidics with machine learning algorithms for RBC classification in rare hereditary hemolytic anemia Subject: Scientific Reports, https://doi.org/10.1038/s41598-021-92747-2 Author: Valeria Rizzuto Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue Jun 22 16:18:30 2021 CEST ModDate: Thu Jun 24 07:19:53 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 3819759 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XTHIQP+Wingdings-Regular Type 1C WinAnsi yes yes yes 387 0 MinionPro-Regular Type 1C Custom yes no yes 67 0 LHQAZM+Corbel-Bold CID TrueType Identity-H yes yes yes 68 0 Corbel2 TrueType WinAnsi yes no yes 69 0 KADZLO+Corbel CID TrueType Identity-H yes yes yes 70 0 MinionPro-Bold Type 1C Custom yes no yes 96 0 MinionPro-It Type 1C Custom yes no yes 97 0 IGTSES+TimesNewRomanPSMT TrueType WinAnsi yes yes yes 211 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 03:08:13 CEST RepresentationInformation: 270616.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:05:03 CEST Size: 3819759 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 474 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: Combining microfluidics with machine learning algorithms for RBC classification in rare hereditary hemolytic anemia Children: Item: Title: Results Children: Item: Title: Samples collection. Item: Title: Experimental process. Children: Item: Title: RBCs separation and experimental solution. Item: Title: Microfluidic unit on a chip and RBCs perfusion. Item: Title: Video analysis. Children: Item: Title: Dataset collection and analysis of images from human RBCs. Children: Item: Title: Two-class problem: healthy vs unhealthy. Item: Title: Four-class problem: healthy vs SCD, THAL, and HS. Item: Title: Discussion Item: Title: Conclusions Item: Title: Methods Children: Item: Title: Experimental process. Children: Item: Title: Samples collection. Item: Title: RBCs separation and experimental solution. Item: Title: Microfluidic unit on a chip fabrication. Item: Title: RBCs perfusion. Item: Title: Video preprocessing. Item: Title: Video analysis. Children: Item: Title: Cell localization and automatic ROI extraction. Item: Title: Deep learning analysis and feature extraction. Item: Title: Feature selection. Children: Item: Title: RBC classification set-up for healthy vs unhealthy. Item: Title: RBC classification set-up for healthy vs SCD, THAL, and HS. Item: Title: References Item: Title: Acknowledgements Info: Title: Combining microfluidics with machine learning algorithms for RBC classification in rare hereditary hemolytic anemia Author: Valeria Rizzuto Subject: Scientific Reports, https://doi.org/10.1038/s41598-021-92747-2 Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue Jun 22 16:48:30 CEST 2021 ModDate: Thu Jun 24 07:19:53 CEST 2021 ID: 0x1d33dc3127ba166af30e248c6d22520f, 0x95d2f1dfb19d73c818f85784ca959edf Filters: FilterPipeline: FlateDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1660 ImageHeight: 1416 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1660 ImageHeight: 986 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1156 ImageHeight: 2605 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1424 ImageHeight: 1058 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1100 ImageHeight: 90 ColorSpace: black is zero BitsPerSample: 1 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Fonts: Type0: Font: BaseFont: LHQAZM+Corbel-Bold Encoding: Identity-H ToUnicode: true Font: BaseFont: KADZLO+Corbel Encoding: Identity-H ToUnicode: true Type1: Font: BaseFont: MinionPro-Bold FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Bold Flags: Serif, Nonsymbolic FontBBox: -319, -360, 1684, 1024 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: MinionPro-It FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-It Flags: Serif, Nonsymbolic, Italic FontBBox: -201, -360, 1684, 1002 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true Font: BaseFont: XTHIQP+Wingdings-Regular FontSubset: true FirstChar: 0 LastChar: 255 FontDescriptor: FontName: XTHIQP+Wingdings-Regular Flags: Nonsymbolic FontBBox: 0, -103, 1359, 439 FontFile3: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: MinionPro-Regular FirstChar: 0 LastChar: 255 FontDescriptor: FontName: MinionPro-Regular Flags: Serif, Nonsymbolic FontBBox: -290, -360, 1684, 989 FontFile3: true EncodingDictionary: BaseEncoding: WinAnsiEncoding Differences: true ToUnicode: true TrueType: Font: BaseFont: IGTSES+TimesNewRomanPSMT FontSubset: true FirstChar: 32 LastChar: 121 FontDescriptor: FontName: IGTSES+TimesNewRomanPSMT Flags: Serif, Nonsymbolic FontBBox: -568, -307, 2046, 1040 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true Font: BaseFont: Corbel2 FirstChar: 0 LastChar: 255 FontDescriptor: FontName: Corbel2 Flags: Nonsymbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Encoding: WinAnsiEncoding ToUnicode: true CIDFontType2: Font: BaseFont: KADZLO+Corbel CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: KADZLO+Corbel Flags: Symbolic FontBBox: -553, -239, 1110, 952 FontFile2: true Font: BaseFont: LHQAZM+Corbel-Bold CIDSystemInfo: Registry: Adobe Registry: Identity Supplement: 0 FontDescriptor: FontName: LHQAZM+Corbel-Bold Flags: Symbolic FontBBox: -556, -239, 1155, 952 FontFile2: true XMP: 2021-06-22T19:48:30+05:30 Springer 2021-06-24T07:19:53+02:00 2021-06-24T07:19:53+02:00 application/pdf https://doi.org/10.1038/s41598-021-92747-2 Nature Publishing Group UK Scientific Reports, https://doi.org/10.1038/s41598-021-92747-2 Combining microfluidics with machine learning algorithms for RBC classification in rare hereditary hemolytic anemia Valeria Rizzuto Arianna Mencattini Begoña Álvarez-González Davide Di Giuseppe Eugenio Martinelli David Beneitez-Pastor Maria del Mar Mañú-Pereira Maria José Lopez-Martinez Josep Samitier 10.1038/s41598-021-92747-2 2010-04-23 true springer.com springerlink.com https://doi.org/10.1038/s41598-021-92747-2 10.1038/s41598-021-92747-2 2045-2322 journal Scientific Reports The Author(s) 2010-04-23 true 10.1038/s41598-021-92747-2 noindex springer.com springerlink.com VoR uuid:19685c71-6d38-474c-a0b2-c95857744c29 uuid:a364afbb-7797-46d6-b8d0-7b46e793b9fa default 1 converted uuid:ed72d49a-ef99-4243-9403-04bebbbca6c6 converted to PDF/A-2b pdfToolbox 2021-06-22T19:49:35+05:30 converted uuid:02aa43ab-8f8a-49c9-aa4c-e26cee6f8b9b converted to PDF/A-2b pdfToolbox 2021-06-22T19:50:21+05:30 Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) 2 B http://ns.adobe.com/pdfx/1.3/ pdfx Adobe Document Info PDF eXtension Schema external Mirrors crossmark:MajorVersionDate CrossmarkMajorVersionDate Text external Mirrors crossmark:CrossmarkDomainExclusive CrossmarkDomainExclusive Text internal Mirrors crossmark:DOI doi Text external Mirrors crossmark:CrosMarkDomains CrossMarkDomains seq Text internal A name object indicating whether the document has been modified to include trapping information robots Text internal ID of PDF/X standard GTS_PDFXVersion Text internal Conformance level of PDF/X standard GTS_PDFXConformance Text internal Company creating the PDF Company Text internal Date when document was last modified SourceModified Text http://crossref.org/crossmark/1.0/ crossmark Crossmark Schema internal Usual same as prism:doi DOI Text external The date when a publication was publishe. MajorVersionDate Text internal CrossmarkDomainExclusive CrossmarkDomainExclusive Text internal CrossMarkDomains CrossMarkDomains seq Text http://prismstandard.org/namespaces/basic/2.0/ prism Prism Schema external This element provides the url for an article or unit of content. The attribute platform is optionally allowed for situations in which multiple URLs must be specified. PRISM recommends that a subset of the PCV platform values, namely “mobile” and “web”, be used in conjunction with this element. NOTE: PRISM recommends against the use of the #other value allowed in the PRISM Platform controlled vocabulary. In lieu of using #other please reach out to the PRISM group at prism-wg@yahoogroups.com to request addition of your term to the Platform Controlled Vocabulary. url URI external The Digital Object Identifier for the article. The DOI may also be used as the dc:identifier. If used as a dc:identifier, the URI form should be captured, and the bare identifier should also be captured using prism:doi. If an alternate unique identifier is used as the required dc:identifier, then the DOI should be specified as a bare identifier within prism:doi only. If the URL associated with a DOI is to be specified, then prism:url may be used in conjunction with prism:doi in order to provide the service endpoint (i.e. the URL). doi Text external ISSN for an electronic version of the issue in which the resource occurs. Permits publishers to include a second ISSN, identifying an electronic version of the issue in which the resource occurs (therefore e(lectronic)Issn. If used, prism:eIssn MUST contain the ISSN of the electronic version. issn Text internal Volume number volume Text internal Issue number number Text internal Starting page startingPage Text internal Ending page endingPage Text external The aggregation type specifies the unit of aggregation for a content collection. Comment PRISM recommends that the PRISM Aggregation Type Controlled Vocabulary be used to provide values for this element. Note: PRISM recommends against the use of the #other value currently allowed in this controlled vocabulary. In lieu of using #other please reach out to the PRISM group at info@prismstandard.org to request addition of your term to the Aggregation Type Controlled Vocabulary. aggregationType Text external Title of the magazine, or other publication, in which a resource was/will be published. Typically this will be used to provide the name of the magazine an article appeared in as metadata for the article, along with information such as the article title, the publisher, volume, number, and cover date. Note: Publication name can be used to differentiate between a print magazine and the online version if the names are different such as “magazine” and “magazine.com.” publicationName Text external Copyright copyright Text http://ns.adobe.com/pdf/1.3/ pdf Adobe PDF Schema internal A name object indicating whether the document has been modified to include trapping information Trapped Text http://ns.adobe.com/xap/1.0/mm/ xmpMM XMP Media Management Schema internal UUID based identifier for specific incarnation of a document InstanceID URI internal The common identifier for all versions and renditions of a document. DocumentID URI internal The common identifier for all versions and renditions of a document. OriginalDocumentID URI internal A reference to the original document from which this one is derived. It is a minimal reference; missing components can be assumed to be unchanged. For example, a new version might only need to specify the instance ID and version number of the previous version, or a rendition might only need to specify the instance ID and rendition class of the original. DerivedFrom ResourceRef Identifies a portion of a document. This can be a position at which the document has been changed since the most recent event history (stEvt:changed). For a resource within an xmpMM:Ingredients list, the ResourceRef uses this type to identify both the portion of the containing document that refers to the resource, and the portion of the referenced resource that is referenced. http://ns.adobe.com/xap/1.0/sType/Part# stPart Part http://www.aiim.org/pdfa/ns/id/ pdfaid PDF/A ID Schema internal Part of PDF/A standard part Integer internal Amendment of PDF/A standard amd Text internal Conformance level of PDF/A standard conformance Text http://ns.adobe.com/xap/1.0/t/pg/ xmpTPg XMP Paged-Text internal XMP08 Spec: An ordered array of plate names that are needed to print the document (including any in contained documents). PlateNames Seq Text http://www.niso.org/schemas/jav/1.0/ jav NISO external Values for Journal Article Version are one of the following: AO = Author’s Original SMUR = Submitted Manuscript Under Review AM = Accepted Manuscript P = Proof VoR = Version of Record CVoR = Corrected Version of Record EVoR = Enhanced Version of Record journal_article_version Closed Choice of Text Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 354, 290, 357, 278 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 358, 290, 361, 278 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 475, 250, 477, 238 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 479, 250, 482, 238 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 219, 210, 222, 198 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 481, 675, 556, 658 Flags: Print AppearanceDictionary: true Thumb: true Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 520, 704, 523, 692 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 263, 624, 266, 612 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 269, 624, 272, 612 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 204, 604, 210, 592 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 350, 594, 355, 582 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 290, 574, 293, 562 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 295, 574, 300, 562 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 302, 574, 307, 562 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 487, 534, 492, 522 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 333, 484, 338, 472 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 191, 424, 196, 412 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 225, 414, 231, 402 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 190, 394, 196, 382 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 217, 364, 223, 352 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 226, 364, 232, 352 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 240, 344, 246, 332 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 398, 334, 404, 322 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 244, 314, 250, 302 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 431, 294, 436, 282 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 192, 274, 198, 262 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 201, 274, 207, 262 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 393, 264, 399, 252 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 510, 234, 515, 222 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 517, 234, 522, 222 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 292, 214, 298, 202 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 299, 214, 305, 202 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 306, 214, 312, 202 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 313, 214, 319, 202 Flags: Print AppearanceDictionary: true Destination: 218 Thumb: true Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 181, 283, 185, 270 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 453, 241, 457, 228 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 445, 209, 449, 196 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 387, 189, 390, 176 Flags: Print AppearanceDictionary: true Destination: 194 Annotation: Subtype: Link Rect: 392, 189, 397, 176 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 375, 117, 380, 104 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 279, 57, 283, 44 Flags: Print AppearanceDictionary: true Destination: 105 Thumb: true Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 469, 169, 473, 157 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 209, 67, 214, 55 Flags: Print AppearanceDictionary: true Destination: 105 Annotation: Subtype: Link Rect: 189, 57, 193, 45 Flags: Print AppearanceDictionary: true Destination: 105 Thumb: true Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 454, 734, 458, 722 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 193, 442, 197, 430 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 302, 412, 306, 400 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 292, 372, 296, 360 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 218, 352, 222, 340 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 201, 332, 205, 320 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 181, 240, 185, 228 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 281, 170, 285, 158 Flags: Print AppearanceDictionary: true Destination: 146 Annotation: Subtype: Link Rect: 448, 110, 453, 98 Flags: Print AppearanceDictionary: true Destination: 153 Thumb: true Page: Sequence: 6 Thumb: true Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 501, 324, 506, 312 Flags: Print AppearanceDictionary: true Destination: 153 Annotation: Subtype: Link Rect: 257, 152, 263, 140 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 262, 72, 268, 60 Flags: Print AppearanceDictionary: true Destination: 218 Thumb: true Page: Sequence: 8 Annotations: Annotation: Subtype: Link Rect: 307, 674, 309, 662 Flags: Print AppearanceDictionary: true Destination: 194 Thumb: true Page: Sequence: 9 Annotations: Annotation: Subtype: Link Rect: 525, 532, 530, 520 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 546, 286, 552, 274 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 174, 266, 179, 254 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 182, 266, 188, 254 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 420, 266, 424, 254 Flags: Print AppearanceDictionary: true Destination: 77 Annotation: Subtype: Link Rect: 197, 154, 203, 142 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 493, 114, 498, 102 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 327, 104, 332, 92 Flags: Print AppearanceDictionary: true Destination: 218 Thumb: true Page: Sequence: 10 Annotations: Annotation: Subtype: Link Rect: 377, 612, 383, 600 Flags: Print AppearanceDictionary: true Destination: 218 Annotation: Subtype: Link Rect: 482, 263, 554, 253 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 169, 255, 198, 245 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 467, 102, 554, 92 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 169, 93, 187, 83 Flags: Print AppearanceDictionary: true Thumb: true Page: Sequence: 11 Annotations: Annotation: Subtype: Link Rect: 192, 665, 332, 655 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 185, 589, 291, 579 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 499, 546, 554, 536 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 169, 538, 216, 528 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 500, 436, 554, 426 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 499, 160, 554, 147 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 155, 149, 260, 137 Flags: Print AppearanceDictionary: true Annotation: Subtype: Link Rect: 351, 120, 443, 107 Flags: Print AppearanceDictionary: true Thumb: true Page: Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 358, 664, 517, 652 Flags: Print AppearanceDictionary: true Thumb: true Checksum: 73913c0a Type: CRC32 Checksum: b466aef752f58e9cdf8fda03d87c17b6 Type: MD5 Checksum: 3549a8e898ebfa1eba37fc9c19e768ac280be336 Type: SHA-1