2b0295d70f5486bd0acd38c45c8f93ad jcm-10-03496.pdf 5875079296a3895664d0462bdcd722f70f44830f jcm-10-03496.pdf 5f3d777d427066de9c55d93e61a0d64066048025ec6963c39cb4d72a664e66d3 jcm-10-03496.pdf Title: Markers of Monocyte Activation, Inflammation, and Microbial Translocation Are Associated with Liver Fibrosis in Alcohol Use Disorder Subject: Background: The association between markers of inflammation (interleukin (IL)-6 and IL-10), monocyte activation (sCD163 and sCD14), and microbial translocation (lipopolysaccharide (LPS) and LPS binding protein) and liver fibrosis in patients with alcohol use disorder (AUD) and no overt liver disease is not well established. Methods: We studied patients admitted for treatment of AUD at two hospitals in Barcelona. Advanced liver fibrosis (ALF) was defined as FIB-4 > 3.25. Results: A total of 353 participants (76.3% male) were included and 94 (26.5%) had ALF. In adjusted correlation analyses, sCD163, sCD14, IL-6, IL-10, and LPS binding protein levels directly correlated with FIB-4 values (adjusted correlation coefficients 0.214, 0.452, 0.317, 0.204, and 0.171, respectively). However, LPS levels were inversely associated with FIB-4 (-0.283). All plasma marker levels in the highest quartile, except LPS, were associated with ALF (sCD163, sCD14, IL-6, IL-10, and LPS binding protein: adjusted odds ratio (aOR) 11.49 (95% confidence interval 6.42–20.56), 1.87 (1.11–3.16), 2.99 (1.79–5.01), 1.84 (1.11–3.16), and 2.13 (1.30–3.50), respectively). Conversely, LPS levels in the lowest quartile were associated with ALF (aOR 2.58 (1.48–4.58), p < 0.01). Conclusion: In AUD patients, plasma levels of the markers of inflammation, monocyte activation, and microbial translocation are associated with ALF. Keywords: alcohol use disorder; monocyte activation; inflammation; microbial translocation; liver fibrosis Author: Daniel Fuster, Xavier Garcia-Calvo, Oriol Farré, Paola Zuluaga, Ferran Bolao, Alba Leis, Anna Hernández-Rubio, Inmaculada Rivas and Robert Muga Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Aug 26 07:57:21 2021 CEST ModDate: Thu Aug 26 08:00:15 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 821341 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FDPKDP+VnURWPalladioL Type 1 Custom yes yes no 10 0 TELWZH+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 UWLJOF+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 RLQMBA+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 SOSCBV+CMSY10 Type 1 Builtin yes yes no 28 0 GIFXFT+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 43 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 65 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 69 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 73 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 91 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 103 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 107 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 111 0 BCDHEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 115 0 BCDKEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 122 0 BCDLEE+Cambria TrueType WinAnsi yes yes no 127 0 BCDMEE+Calibri TrueType WinAnsi yes yes no 131 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-06-21 02:49:59 CEST RepresentationInformation: jcm-10-03496.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2024-06-20 16:11:59 CEST Size: 821341 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 291 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Participants Destination: subsection.2.1 Item: Title: Measurements Destination: subsection.2.2 Item: Title: Statistical Analysis Destination: subsection.2.3 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Markers of Monocyte Activation, Inflammation, and Microbial Translocation Are Associated with Liver Fibrosis in Alcohol Use Disorder Author: Daniel Fuster, Xavier Garcia-Calvo, Oriol Farré, Paola Zuluaga, Ferran Bolao, Alba Leis, Anna Hernández-Rubio, Inmaculada Rivas and Robert Muga Subject: Background: The association between markers of inflammation (interleukin (IL)-6 and IL-10), monocyte activation (sCD163 and sCD14), and microbial translocation (lipopolysaccharide (LPS) and LPS binding protein) and liver fibrosis in patients with alcohol use disorder (AUD) and no overt liver disease is not well established. Methods: We studied patients admitted for treatment of AUD at two hospitals in Barcelona. Advanced liver fibrosis (ALF) was defined as FIB-4 > 3.25. Results: A total of 353 participants (76.3% male) were included and 94 (26.5%) had ALF. In adjusted correlation analyses, sCD163, sCD14, IL-6, IL-10, and LPS binding protein levels directly correlated with FIB-4 values (adjusted correlation coefficients 0.214, 0.452, 0.317, 0.204, and 0.171, respectively). However, LPS levels were inversely associated with FIB-4 (-0.283). All plasma marker levels in the highest quartile, except LPS, were associated with ALF (sCD163, sCD14, IL-6, IL-10, and LPS binding protein: adjusted odds ratio (aOR) 11.49 (95% confidence interval 6.42 20.56), 1.87 (1.11 3.16), 2.99 (1.79 5.01), 1.84 (1.11 3.16), and 2.13 (1.30 3.50), respectively). Conversely, LPS levels in the lowest quartile were associated with ALF (aOR 2.58 (1.48 4.58), p < 0.01). Conclusion: In AUD patients, plasma levels of the markers of inflammation, monocyte activation, and microbial translocation are associated with ALF. 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