c9f6a7f8f1e5fdd1117a0e197275d492 pmc_33654150.pdf 6e84d52d250914a819787df95646c7003748bc72 pmc_33654150.pdf 15183bce3589c35312bf870145a34e47a08943216568482949dc85d4de54fb47 pmc_33654150.pdf Title: First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract Subject: Scientific Reports, https://doi.org/10.1038/s41598-021-84303-9 Author: Jessica Calo Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed Feb 24 03:28:56 2021 CET ModDate: Wed Feb 24 04:49:31 2021 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2061843 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- QTIRCO+Wingdings-Regular Type 1C WinAnsi yes yes yes 386 0 MinionPro-It Type 1C Custom yes no yes 76 0 MinionPro-Regular Type 1C Custom yes no yes 75 0 CIOYPU+Corbel-BoldItalic CID TrueType Identity-H yes yes yes 387 0 NGHRNY+Corbel-Bold CID TrueType Identity-H yes yes yes 77 0 Corbel2 TrueType WinAnsi yes no yes 78 0 SDRVFO+Corbel CID TrueType Identity-H yes yes yes 79 0 SDRVFO+Corbel CID TrueType Identity-H yes yes yes 426 0 MinionPro-Bold Type 1C Custom yes no yes 105 0 Corbel2 TrueType WinAnsi yes no yes 109 0 LMCJLC+STIXMath-Regular Type 1C Custom yes yes yes 323 0 LMCJLC+TimesNewRomanPSMT CID TrueType Identity-H yes yes yes 193 0 TimesNewRomanPSMT2 TrueType WinAnsi yes no yes 278 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:40:34 CEST RepresentationInformation: pmc_33654150.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:05:27 CEST Size: 2061843 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 464 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract Children: Item: Title: Results Children: Item: Title: Amino acid receptors mRNAs are present in the rainbow trout gastrointestinal tract. Item: Title: Luminal amino acids modulates amino acid receptors and intracellular signaling molecules mRNAs. Item: Title: Gut hormones are differentially expressed along the rainbow trout GIT. Item: Title: Gene and protein abundance of gut hormones respond to luminal amino acids. Item: Title: Discussion Item: Title: Material and methods Children: Item: Title: Animals. Item: Title: Experimental designs. Children: Item: Title: Study of amino acid receptors and gut hormones mRNA distribution along the GIT. Item: Title: Study of the response of amino acid receptors to the luminal presence of amino acids. Item: Title: Assessment of plasma metabolite levels. Item: Title: Quantification of mRNA abundance by real-time PCR. Item: Title: Analysis of protein levels by Western blot. Item: Title: Statistical analysis. Item: Title: References Item: Title: Acknowledgements Info: Title: First evidence for the presence of amino acid sensing mechanisms in the fish gastrointestinal tract Author: Jessica Calo Subject: Scientific Reports, https://doi.org/10.1038/s41598-021-84303-9 Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Wed Feb 24 03:58:56 CET 2021 ModDate: Wed Feb 24 04:49:31 CET 2021 ID: 0x8d2dc35a3934d56538ed6f7fb65297e1, 0x5b5b73b577174c9c337a19b3f26bb382 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 2002 ImageHeight: 1155 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Intent: RelativeColorimetric Name: X Image: NisoImageMetadata: FormatName: image/png ImageWidth: 1436 ImageHeight: 2593 ColorSpace: black is zero BitsPerSample: 8 BitsPerSampleUnit: integer Filter: 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