e76a9c3f35ec64773299c5b8bf26c9d1 pmc_34577085.pdf 5e9f749805cf01b3f39c5dce169b1cfccd6e76c9 pmc_34577085.pdf 79517d6e82420fd3287b576619c662f4254dd05ab4d4fda0855d38aaa98ff584 pmc_34577085.pdf Title: Selenite Downregulates STAT3 Expression and Provokes Lymphocytosis in the Liver of Chronically Exposed Syrian Golden Hamsters Subject: Arsenic is considered a worldwide pollutant that can be present in drinking water. Arsenic exposure is associated with various diseases, including cancer. Antioxidants as selenite and -tocopherol-succinate have been shown to modulate arsenic toxic effects. Since changes in STAT3 and PSMD10 gene expression have been associated with carcinogenesis, the aim of this study was to evaluate the effect of arsenic exposure and co-treatments with selenite or -tocopherol-succinate on the expression of these genes, in the livers of chronically exposed Syrian golden hamsters. Animals were divided into six groups: (i) control, (ii) chronically treated with 100 ppm arsenic, (iii) treated with 6 ppm -tocopherol-succinate (-TOS), (iv) treated with 8.5 ppm selenite, (v) treated witharsenic + -TOS, and (vi) treated with arsenic + selenite. Urine samples and livers were collected after 20 weeks of continuous exposure. The urine samples were analyzed for arsenic species by atomic absorption spectrophotometry, and real-time RT-qPCR analysis was performed for gene expression evaluation. A reduction in STAT3 expression was observed in the selenite-treated group. No differences in PSMD10 expression were found among groups. Histopathological analysis revealed hepatic lymphocytosis in selenite-treated animals. As a conclusion, long-term exposure to arsenic does not significantly alter the expression of STAT3 and PSMD10 oncogenes in the livers of hamsters; however, selenite down-regulates STAT3 expression and provokes lymphocytosis. Keywords: arsenic; selenite; -tocopherol; liver; hamster; oncogenes Author: María Elena Camacho-Moll, Adriana Sampayo-Reyes, Fabiola Castorena-Torres, Gerardo Lozano-Garza, Gabriela Alarcón-Galván, Alba Hernández, Ricard Marcos, Juan Manuel Alcocer-González, Reyes Tamez-Guerra and Mario Bermúdez de León Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Sep 16 10:45:25 2021 CEST ModDate: Thu Sep 16 11:02:01 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 12 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2060274 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- AWHDWW+VnURWPalladioL Type 1 Custom yes yes no 10 0 ZXDKIP+EURM10 Type 1 Builtin yes yes no 15 0 KADPNJ+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 BVQFGT+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 TNFYJA+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 JCYIDT+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 PUSVWS+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 YFFKLH+CMSY10 Type 1 Builtin yes yes no 85 0 DHHFHA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 96 0 DHHFHB+PalatinoLinotype CID TrueType Identity-H yes yes yes 99 0 DHHFHC+PalatinoLinotype TrueType WinAnsi yes yes no 105 0 DHHFIE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 108 0 DHHFHA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 130 0 DHHFHB+PalatinoLinotype CID TrueType Identity-H yes yes yes 133 0 DHHFHC+PalatinoLinotype TrueType WinAnsi yes yes no 139 0 DHHFIE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 142 0 DHHFHA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 159 0 DHHFHC+PalatinoLinotype TrueType WinAnsi yes yes no 162 0 DHHFIE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 165 0 DHHFHA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 177 0 DHHFHC+PalatinoLinotype TrueType WinAnsi yes yes no 180 0 DHHFIE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 183 0 URYLRZ+URWPalladioL-BoldItal Type 1 Custom yes yes no 194 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 201 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2022-03-22 02:44:31 CET RepresentationInformation: pmc_34577085.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-10-04 03:36:06 CEST Size: 2060274 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 406 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Children: Item: Title: Chemicals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Animals and Treatments Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Measurement of Arsenic and Arsenic Species Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: RNA Extraction and cDNA Synthesis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: SYBR Green Quantitative Real-Time Reverse Transcriptase (RT)-PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Hematoxylin and Eosin Staining Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Data Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Info: Title: Selenite Downregulates STAT3 Expression and Provokes Lymphocytosis in the Liver of Chronically Exposed Syrian Golden Hamsters Author: María Elena Camacho-Moll, Adriana Sampayo-Reyes, Fabiola Castorena-Torres, Gerardo Lozano-Garza, Gabriela Alarcón-Galván, Alba Hernández, Ricard Marcos, Juan Manuel Alcocer-González, Reyes Tamez-Guerra and Mario Bermúdez de León Subject: Arsenic is considered a worldwide pollutant that can be present in drinking water. Arsenic exposure is associated with various diseases, including cancer. Antioxidants as selenite and -tocopherol-succinate have been shown to modulate arsenic toxic effects. Since changes in STAT3 and PSMD10 gene expression have been associated with carcinogenesis, the aim of this study was to evaluate the effect of arsenic exposure and co-treatments with selenite or -tocopherol-succinate on the expression of these genes, in the livers of chronically exposed Syrian golden hamsters. Animals were divided into six groups: (i) control, (ii) chronically treated with 100 ppm arsenic, (iii) treated with 6 ppm -tocopherol-succinate (-TOS), (iv) treated with 8.5 ppm selenite, (v) treated witharsenic + -TOS, and (vi) treated with arsenic + selenite. Urine samples and livers were collected after 20 weeks of continuous exposure. The urine samples were analyzed for arsenic species by atomic absorption spectrophotometry, and real-time RT-qPCR analysis was performed for gene expression evaluation. A reduction in STAT3 expression was observed in the selenite-treated group. No differences in PSMD10 expression were found among groups. Histopathological analysis revealed hepatic lymphocytosis in selenite-treated animals. As a conclusion, long-term exposure to arsenic does not significantly alter the expression of STAT3 and PSMD10 oncogenes in the livers of hamsters; however, selenite down-regulates STAT3 expression and provokes lymphocytosis. 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