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Keywords: DNA bridges, genomic instability, chromosome damage, mitosis, DNA repair Author: Anna Genescà and Teresa Anglada Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.15 CreationDate: Wed Sep 22 14:50:45 2021 CEST ModDate: Wed Sep 22 17:54:14 2021 CEST Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 779.528 pts Page rot: 0 File size: 4434450 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BYECFY+MinionPro-Regular Type 1 Custom yes yes no 24 0 JLVCLD+HelveticaNeueLTStd-Bd Type 1 Custom yes yes no 25 0 MIMIGL+HelveticaNeueLTStd-Lt Type 1 Custom yes yes no 26 0 CCVXXJ+HelveticaNeueLTStd-BdIt Type 1 Custom yes yes no 31 0 KTDVCQ+HelveticaNeueLTStd-LtIt Type 1 Custom yes yes no 32 0 EYKBQG+HelveticaNeueLTStd-MdIt Type 1 Custom yes yes no 33 0 TNRPCD+MTSYN Type 1 Builtin yes yes no 34 0 MISHUY+MTGU Type 1 Builtin yes yes no 109 0 NADRNU+MinionPro-It Type 1 Custom yes yes no 113 0 YKRAVM+MinionPro-Bold Type 1 Custom yes yes no 148 0 XODHDR+RBLMI Type 1 Builtin yes yes no 182 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-11-23 05:27:19 CET RepresentationInformation: pmc_34650988.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:05:53 CEST Size: 4434450 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 738 FreeObjects: 3 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Breakage of CRISPR/Cas9-Induced Chromosome Bridges in Mitotic Cells Children: Item: Title: Introduction Item: Title: Materials and Methods Children: Item: Title: Cell Culture Item: Title: Plasmid Construction Item: Title: Viral Particle Production and Transduction Item: Title: Methods to Generate Chromosome Bridges Item: Title: BrdU Incorporation Item: Title: Immunofluorescence Item: Title: Fluorescence in situ Hybridization Item: Title: SensiTive Recognition of Individual DNA Ends Item: Title: Live-Cell Imaging Item: Title: Statistics Item: Title: Results Children: Item: Title: The Frequency of Chromosome Bridges Decreases as Cells Exit Mitosis and Progress to Interphase Item: Title: Breakage of Chromosome Bridges During Mitosis Item: Title: DNA Ends in Mitotic Chromosome Bridges Are Sensed as Broken Ends Item: Title: DNA Ends in Mitotic Chromatin Bridges Are Not Processed for Repair Item: Title: Breakage of Bridges in Mitotic Cells Is Associated With their Stretching Item: Title: Discussion Children: Item: Title: The Ends of Broken Chromosome Bridges in Mitotic Cells Are Detected but Not Repaired Item: Title: Intense Stretching and Constriction of the Condensed Chromosome of Bridges in Dividing Cells Item: Title: Data Availability Statement Item: Title: Author Contributions Item: Title: Funding Item: Title: Acknowledgments Item: Title: Supplementary Material Item: Title: References Info: Title: Breakage of CRISPR/Cas9-Induced Chromosome Bridges in Mitotic Cells Author: Anna Genescà and Teresa Anglada Subject: Chromosomal instability, the most frequent form of plasticity in cancer cells, often proceeds through the formation of chromosome bridges. 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