04226ecf839697f886b9e1812e001932 pmc_34679937.pdf 1257c12ec3b6b1e7e66d320d59109411908230ca pmc_34679937.pdf 927e982169b8b40835036efdab2fa2b070ddfa8634995ee39038838ae219d696 pmc_34679937.pdf Title: Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis Subject: The aim of this study was to standardize a simple, manual platelet-rich plasma (PRP) protocol in Catalonian donkeys using single-spin tube centrifugation as a treatment for jenny endometritis. The objective was to obtain a blood product with a moderate concentration of platelets (2 or 3 times baseline physiologic values) and a low WBC (White Blood Cells) concentration. Blood was drawn from six Catalonian donkeys using acid citrate dextrose (ACD) as an anticoagulant, and then processed by single centrifugation at 133 g for two different centrifugation times (10 and 15 min). The PRP samples were evaluated by flow cytometry, and TGF-1 (Transforming Growth Factor-Beta1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The 10 min centrifugation protocol resulted in a slightly greater release of TGF-1 (6044.79 ng/mL), a 2.06-fold increase in platelet concentration, and a 15-fold reduction in leukocyte concentration when compared to the initial values. The 15 min centrifugation time resulted in a 2.44-fold increase in baseline platelet concentration, a reduction in WBC count by a factor of 20, and slightly lower TGF levels (5206 ng/mL). We conclude that both protocols are adequate for the obtention of PRP, and both may have an acceptable therapeutic potential for use in this species, although this needs to be further validated. Keywords: donkey; platelet-rich plasma (PRP); ACD; TGF-1 Author: Priscila Fantini, Román Jiménez, Karina Vilés, Antoni Iborra, Maristela Silveira Palhares, Jaime Catalán, Marta Prades and Jordi Miró Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Oct 13 11:22:23 2021 CEST ModDate: Wed Oct 13 11:33:52 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 463889 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DQHEJR+VnURWPalladioL Type 1 Custom yes yes no 10 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 15 0 ZNJAUQ+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 NVUTDZ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 MDZMJH+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 32 0 ZZTQUO+VnURWPalladioL-Bold Type 1 Custom yes yes no 36 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 44 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 66 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 70 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 74 0 IFGGML+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 96 0 IFGGMN+PalatinoLinotype TrueType WinAnsi yes yes no 99 0 IFGGMP+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 102 0 TZZUPX+URWPalladioL-BoldItal Type 1 Custom yes yes no 112 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-11-23 05:27:13 CET RepresentationInformation: pmc_34679937.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:05:54 CEST Size: 463889 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 270 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animals Destination: subsection.2.1 Item: Title: PRP Preparation Destination: subsection.2.2 Item: Title: Platelet Count and Evaluation Destination: subsection.2.3 Item: Title: Growth Factor Concentration Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Blood Cell Count and Growth Factors Determination Destination: subsection.3.1 Item: Title: Preliminary “In Vivo” Experience Destination: subsection.3.2 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Simple Tube Centrifugation Method for Platelet-Rich Plasma (PRP) Preparation in Catalonian Donkeys as a Treatment of Endometritis-Endometrosis Author: Priscila Fantini, Román Jiménez, Karina Vilés, Antoni Iborra, Maristela Silveira Palhares, Jaime Catalán, Marta Prades and Jordi Miró Subject: The aim of this study was to standardize a simple, manual platelet-rich plasma (PRP) protocol in Catalonian donkeys using single-spin tube centrifugation as a treatment for jenny endometritis. The objective was to obtain a blood product with a moderate concentration of platelets (2 or 3 times baseline physiologic values) and a low WBC (White Blood Cells) concentration. Blood was drawn from six Catalonian donkeys using acid citrate dextrose (ACD) as an anticoagulant, and then processed by single centrifugation at 133 g for two different centrifugation times (10 and 15 min). The PRP samples were evaluated by flow cytometry, and TGF-1 (Transforming Growth Factor-Beta1) concentrations were determined by enzyme-linked immunosorbent assay (ELISA). The 10 min centrifugation protocol resulted in a slightly greater release of TGF-1 (6044.79 ng/mL), a 2.06-fold increase in platelet concentration, and a 15-fold reduction in leukocyte concentration when compared to the initial values. The 15 min centrifugation time resulted in a 2.44-fold increase in baseline platelet concentration, a reduction in WBC count by a factor of 20, and slightly lower TGF levels (5206 ng/mL). We conclude that both protocols are adequate for the obtention of PRP, and both may have an acceptable therapeutic potential for use in this species, although this needs to be further validated. 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