c75292cd0911e2093fbc84a8953cc474 pmc_34769254.pdf 0edfb1c22eb58e6494b354ca8ff5171356d53f6d pmc_34769254.pdf 2948098b70506b9ca13b424e68a66898f70f016e450b53dacb9af7097e41c77a pmc_34769254.pdf Title: Immunomodulatory Lectin-like Peptides for Fish Erythrocytes-Targeting as Potential Antiviral Drug Delivery Platforms Subject: One of the challenges of science in disease prevention is optimizing drug and vaccine delivery. Until now, many strategies have been employed in this sector, but most are quite complex and labile. To overcome these limitations, great efforts are directed to coupling drugs to carriers, either of natural or synthetic origin. Among the most studied cell carriers are antigen-presenting cells (APCs), however, red blood cells (RBCs) are positioned as attractive carriers in drug delivery due to their abundance and availability in the body. Furthermore, fish RBCs have a nucleus and have been shown to have a strong involvement in modulating the immune response. In this study, we evaluated the binding of three peptides to rainbow trout RBCs, two lectin-like peptides and another derived from Plasmodium falciparum membrane protein, in order to take advantage of this peptide-RBCs binding to generate tools to improve the specificity, efficacy, immunostimulatory effect, and safety of the antiviral therapeutic or prophylactic administration systems currently used. Keywords: peptide; red blood cells; erythrocyte; drug delivery; ligand; prophylactic; therapeutic; cell-targeting; lectin; fish Author: Maria Salvador-Mira, Veronica Chico, Monica Arostica, Fanny Guzmán, Nerea Roher, Luis Perez and Maria del Mar Ortega-Villaizan Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Nov 4 06:44:02 2021 CET ModDate: Thu Nov 4 06:48:49 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 4547322 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ARIQJE+VnURWPalladioL Type 1 Custom yes yes no 10 0 DWYLWW+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 FRWJVR+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 VVAGJC+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 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yes no 156 0 GAFLIK+PalatinoLinotype,Italic CID TrueType Identity-H yes yes yes 159 0 GAFLIL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 165 0 GAFKNL+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 179 0 GAFLIJ+PalatinoLinotype TrueType WinAnsi yes yes no 182 0 GAFLIL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 185 0 BCDEEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 217 0 BCDEEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 229 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 240 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 02:55:07 CEST RepresentationInformation: pmc_34769254.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:06:08 CEST Size: 4547322 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 498 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: In Vitro Evaluation of Peptide Binding to Rainbow Trout RBCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: In Vitro Analysis of Gene Expression in Rainbow Trout RBCs after Peptide Treatment Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: In Vivo Evaluation of Peptide Binding to Rainbow Trout RBCs Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Children: Item: Title: Peptide Synthesis and Characterization Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Animals Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: RBCs Purification and In Vitro Treatment with the Peptides Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Peripheral Blood Sampling after In Vivo Injection with the Peptides Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: RNA Isolation and Gene Expression by RT-qPCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Flow Cytometer Assays Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Fluorescent Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Confocal Microscopy Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Software Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Info: Title: Immunomodulatory Lectin-like Peptides for Fish Erythrocytes-Targeting as Potential Antiviral Drug Delivery Platforms Author: Maria Salvador-Mira, Veronica Chico, Monica Arostica, Fanny Guzmán, Nerea Roher, Luis Perez and Maria del Mar Ortega-Villaizan Subject: One of the challenges of science in disease prevention is optimizing drug and vaccine delivery. Until now, many strategies have been employed in this sector, but most are quite complex and labile. To overcome these limitations, great efforts are directed to coupling drugs to carriers, either of natural or synthetic origin. Among the most studied cell carriers are antigen-presenting cells (APCs), however, red blood cells (RBCs) are positioned as attractive carriers in drug delivery due to their abundance and availability in the body. Furthermore, fish RBCs have a nucleus and have been shown to have a strong involvement in modulating the immune response. In this study, we evaluated the binding of three peptides to rainbow trout RBCs, two lectin-like peptides and another derived from Plasmodium falciparum membrane protein, in order to take advantage of this peptide-RBCs binding to generate tools to improve the specificity, efficacy, immunostimulatory effect, and safety of the antiviral therapeutic or prophylactic administration systems currently used. 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