d5ab3fc81c003a35caf0c192ab94a2cb pmc_34943483.pdf 7c47ab3b6455cc2ef5a26d1c6ba97d560d5b3a12 pmc_34943483.pdf 82cd304ed999ecba31d64d6b7a9f25a2844222f53e2b4550c7365b7d35962962 pmc_34943483.pdf Title: Profiling of Myositis Specific Antibodies and Composite Scores as an Aid in the Differential Diagnosis of Autoimmune Myopathies Subject: (1) Background: Myositis specific antibodies (MSA) represent important diagnostic and stratification tools in idiopathic inflammatory myositis (IIM) patients. Here we aimed to evaluate the clinical performance of MSA profiled by a novel particle based multi-analyte technology (PMAT) in IIM and subsets thereof. (2) Methods: 264 IIM patients and 200 controls were tested for MSA using PMAT (Inova Diagnostics, research use only). Diagnostic performance was analyzed and composite scores were generated. (3) Results: The sensitivity/specificity of the individual MSA were: 19.7%/100% (Jo-1), 7.2%/100.0% (Mi-2), 3.0%/99.0% (NXP2), 3.8%/100.0% (SAE), 2.7%/100.0% (PL-7), 1.9%/99.5 (PL-12), 1.1%/100.0% (EJ), 15.5%/99.5% (TIF1), 8.3%/98.5% (MDA5), 6.1%/99.0% (HMGCR) and 1.9%/98.5% (SRP). Of all IIM patients, 180/264 tested positive for at least one of the MSAs. In the individual control group, 12/200 (6.0%) tested positive for at least one MSA, most of which had levels close to the cut-off (except one SRP and one PL-12). Only 6/264 (2.3%) IIM patients were positive for more than one antibody (MDA5/HMGCR, EJ/PL-7, 2 MDA5/TIF1, EJ/SAE, SAE/TIF1). The overall sensitivity was 68.2% paired with a specificity of 94.0%, leading to an odds ratio of 33.8. The composite scores showed good discrimination between subgroups (e.g., anti-synthetase syndrome). (4) Conclusion: MSA, especially when combined in composite scores (here measured by PMAT), provide value in stratification of patients with IIM. Keywords: myositis; autoantibodies; diagnosis; polymyositis; dermatomyositis; immunoassay Author: Michael Mahler, Kishore Malyavantham, Andrea Seaman, Chelsea Bentow, Ariadna Anunciacion-Llunell, María Teresa Sanz-Martínez, Laura Viñas-Gimenez and Albert Selva-O’Callaghan Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Dec 2 02:12:47 2021 CET ModDate: Thu Dec 2 02:17:43 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2987384 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- CIQLLF+VnURWPalladioL Type 1 Custom yes yes no 10 0 MKDWFJ+EURM10 Type 1 Builtin yes yes no 15 0 HUHJFM+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 WVCJMJ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 SMPHDE+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 IXLVLQ+CMSY10 Type 1 Builtin yes yes no 32 0 WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 36 0 BXZUUH+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 44 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 66 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 70 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 74 0 BPFEFI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 105 0 BPFEFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 108 0 BPFEFK+PalatinoLinotype TrueType WinAnsi yes yes no 114 0 BPFEGM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 117 0 BPFFLF+Cambria CID TrueType Identity-H yes yes yes 120 0 BPFEFI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 143 0 BPFEFK+PalatinoLinotype TrueType WinAnsi yes yes no 146 0 BPFEGM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 149 0 BPFFLF+Cambria CID TrueType Identity-H yes yes yes 152 0 BPFEFI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 170 0 BPFEFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 173 0 BPFEFK+PalatinoLinotype TrueType WinAnsi yes yes no 179 0 BPFEGM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 182 0 BPFEFI+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 202 0 BPFEFJ+PalatinoLinotype CID TrueType Identity-H yes yes yes 205 0 BPFEFK+PalatinoLinotype TrueType WinAnsi yes yes no 211 0 BPFEGM+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 214 0 BPFNLF+Calibri CID TrueType Identity-H yes yes yes 217 0 BPFNNF+Calibri,Bold CID TrueType Identity-H yes yes yes 223 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:37:45 CEST RepresentationInformation: pmc_34943483.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:06:32 CEST Size: 2987384 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 439 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Patient Cohort Destination: subsection.2.1 Item: Title: Autoantibody Testing Destination: subsection.2.2 Item: Title: Statistical Methods Destination: subsection.2.3 Item: Title: Results Destination: section.3 Children: Item: Title: Prevalence of Myositis Specific Antibodies in Myositis and Controls Destination: subsection.3.1 Item: Title: Quantitative Analysis of Myositis Specific Antibodies Studied Destination: subsection.3.2 Item: Title: Prevalence of Myositis Specific Antibodies in Myositis Subsets Destination: subsection.3.3 Item: Title: Generation and Performance Assessment of MyoScores for Idiopathic Inflammatory Myopathies Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Profiling of Myositis Specific Antibodies and Composite Scores as an Aid in the Differential Diagnosis of Autoimmune Myopathies Author: Michael Mahler, Kishore Malyavantham, Andrea Seaman, Chelsea Bentow, Ariadna Anunciacion-Llunell, María Teresa Sanz-Martínez, Laura Viñas-Gimenez and Albert Selva-O’Callaghan Subject: (1) Background: Myositis specific antibodies (MSA) represent important diagnostic and stratification tools in idiopathic inflammatory myositis (IIM) patients. Here we aimed to evaluate the clinical performance of MSA profiled by a novel particle based multi-analyte technology (PMAT) in IIM and subsets thereof. (2) Methods: 264 IIM patients and 200 controls were tested for MSA using PMAT (Inova Diagnostics, research use only). Diagnostic performance was analyzed and composite scores were generated. (3) Results: The sensitivity/specificity of the individual MSA were: 19.7%/100% (Jo-1), 7.2%/100.0% (Mi-2), 3.0%/99.0% (NXP2), 3.8%/100.0% (SAE), 2.7%/100.0% (PL-7), 1.9%/99.5 (PL-12), 1.1%/100.0% (EJ), 15.5%/99.5% (TIF1), 8.3%/98.5% (MDA5), 6.1%/99.0% (HMGCR) and 1.9%/98.5% (SRP). Of all IIM patients, 180/264 tested positive for at least one of the MSAs. In the individual control group, 12/200 (6.0%) tested positive for at least one MSA, most of which had levels close to the cut-off (except one SRP and one PL-12). Only 6/264 (2.3%) IIM patients were positive for more than one antibody (MDA5/HMGCR, EJ/PL-7, 2 MDA5/TIF1, EJ/SAE, SAE/TIF1). The overall sensitivity was 68.2% paired with a specificity of 94.0%, leading to an odds ratio of 33.8. The composite scores showed good discrimination between subgroups (e.g., anti-synthetase syndrome). (4) Conclusion: MSA, especially when combined in composite scores (here measured by PMAT), provide value in stratification of patients with IIM. 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