096ac660d95071918d3135cc9999c195 pmc_34944608.pdf 13bc011a69a77a7e4a8e140c095d64b078496a3d pmc_34944608.pdf b7900ade054dcdf3d187b64ad6b72204d4759e14a77b5f08cd46454312908332 pmc_34944608.pdf Title: Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging Subject: Glaucoma causes blindness due to the progressive death of retinal ganglion cells. The immune response chronically and subclinically mediates a homeostatic role. In current clinical practice, it is impossible to analyse neuroinflammation non-invasively. However, analysis of vitreous images using optical coherence tomography detects the immune response as hyperreflective opacities. This study monitors vitreous parainflammation in two animal models of glaucoma, comparing both healthy controls and sexes over six months. Computational analysis characterizes in vivo the hyperreflective opacities, identified histologically as hyalocyte-like Iba-1+ (microglial marker) cells. Glaucomatous eyes showed greater intensity and number of vitreous opacities as well as dynamic fluctuations in the percentage of activated cells (50–250 microns2) vs. non-activated cells (10–50 microns2), isolated cells (10 microns2) and complexes (>250 microns2). Smaller opacities (isolated cells) showed the highest mean intensity (intracellular machinery), were the most rounded at earlier stages (recruitment) and showed the greatest change in orientation (motility). Study of vitreous parainflammation could be a biomarker of glaucoma onset and progression. Keywords: optical coherence tomography; biomedical imaging; image analysis and processing; vitreous body; glaucoma; animal models; inflammation Author: María Jesús Rodrigo, Manuel Subías, Alberto Montolío, Silvia Méndez-Martínez, Teresa Martínez-Rincón, Lorena Arias, David García-Herranz, Irene Bravo-Osuna, Julian Garcia-Feijoo, Luis Pablo, José Cegoñino, Rocio Herrero-Vanrell, Ana Carretero, Jesus Ruberte, Elena Garcia-Martin and Amaya Pérez del Palomar Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Nov 30 02:11:54 2021 CET ModDate: Tue Nov 30 02:15:21 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 27 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5655179 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- IEFSKB+VnURWPalladioL Type 1 Custom yes yes no 10 0 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Ms Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Healthy Control Cohort Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: Histology Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: In Vivo Analysis of Vitreous Immunity Detected Using OCT Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Children: Item: Title: Quantification of the Mean Number of Opacities/Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Percentage of Opacities/Cells by Size Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Mean Intensity of Opacities/Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Average Eccentricity of the Opacities/Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Mean Orientation of the Opacities/Cells Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Info: Title: Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging Author: María Jesús Rodrigo, Manuel Subías, Alberto Montolío, Silvia Méndez-Martínez, Teresa Martínez-Rincón, Lorena Arias, David García-Herranz, Irene Bravo-Osuna, Julian Garcia-Feijoo, Luis Pablo, José Cegoñino, Rocio Herrero-Vanrell, Ana Carretero, Jesus Ruberte, Elena Garcia-Martin and Amaya Pérez del Palomar Subject: Glaucoma causes blindness due to the progressive death of retinal ganglion cells. The immune response chronically and subclinically mediates a homeostatic role. In current clinical practice, it is impossible to analyse neuroinflammation non-invasively. However, analysis of vitreous images using optical coherence tomography detects the immune response as hyperreflective opacities. This study monitors vitreous parainflammation in two animal models of glaucoma, comparing both healthy controls and sexes over six months. Computational analysis characterizes in vivo the hyperreflective opacities, identified histologically as hyalocyte-like Iba-1+ (microglial marker) cells. Glaucomatous eyes showed greater intensity and number of vitreous opacities as well as dynamic fluctuations in the percentage of activated cells (50 250 microns2) vs. non-activated cells (10 50 microns2), isolated cells (10 microns2) and complexes (>250 microns2). Smaller opacities (isolated cells) showed the highest mean intensity (intracellular machinery), were the most rounded at earlier stages (recruitment) and showed the greatest change in orientation (motility). Study of vitreous parainflammation could be a biomarker of glaucoma onset and progression. 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