9ec17d77f17ed049bb49114b2acfb6e6 msf-08-00009.pdf 83f791081cbf07c3ff4cb1c67a884de902dd7350 msf-08-00009.pdf 8b391cf35d8fa6c5fa685f15d577ae8c0d6412048bbfeaa09077ff0afccb7e8d msf-08-00009.pdf Title: Measuring Temporal Patterns of the Nest-Building Process in Mice for Animal Welfare and Disease Monitoring "2279 Subject: Nesting behavior in rodents, used to assess animal welfare/illness and instrumental tasks, is also proposed as valuable for disease monitoring, evaluating potential risk factors and interventions. The reliability of Deacon’s 5-point ordinal scale to score nests at 24 h is well-recognized. However, previous work with the 3xTg-AD mice model of Alzheimer’s disease proposed a 3-day protocol to discard false negatives, thus unveiling genotype-, sex- and age-dependent differences. Here, we propose the size of nesting as a numeric variable, complementary to the ordinal scale, to allow parametric repeated measures analysis for identifying and evaluating temporal patterns in the nest-building process. Thus, nests of male and female mice with normal and AD-pathological aging ‘measured’ during 3-days showed that the nest-building process responded to a linear equation in wild-type animals or when female sex was considered but disrupted in males or the AD-genotype. Genotype per sex interaction indicated the optimal nest-building process in wild-type females, as they build the best nests at 72 h and the worst nests in 3xTg-AD mice at 48 h. On each day, data were consistent with the ordinal scale, but the identification of temporal patterns with the numeric variable confirmed nest-building as a complex process, which is sensitive to sex and genotype. Keywords: environment; social; nesting; daily life activities; animal welfare; disease monitoring;aging Author: Lydia Giménez-Llort and Ana María Ruiz de Molina-García Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Aug 5 10:29:47 2022 CEST ModDate: Fri Aug 5 10:35:45 2022 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 5 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 620848 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 LZJWOX+VnURWPalladioL Type 1 Custom yes yes no 15 0 QGYXGD+URWPalladioL-Bold Type 1 Custom yes yes no 19 0 LOCJDD+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 CGLLWB+URWPalladioL-Ital Type 1 Custom yes yes no 28 0 VYVZGZ+CMSY10 Type 1 Builtin yes yes no 72 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 84 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 88 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 92 0 BCDIEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 96 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2025-02-20 02:15:31 CET RepresentationInformation: msf-08-00009.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2025-02-19 10:17:31 CET Size: 620848 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 191 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Animal Model of Alzheimer’s Disease Destination: subsection.2.1 Item: Title: Experimental Subjects and Design Destination: subsection.2.2 Item: Title: Behavioral Assessment Destination: subsection.2.3 Item: Title: Statistics Destination: subsection.2.4 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Measuring Temporal Patterns of the Nest-Building Process in Mice for Animal Welfare and Disease Monitoring "2279 Author: Lydia Giménez-Llort and Ana María Ruiz de Molina-García Subject: Nesting behavior in rodents, used to assess animal welfare/illness and instrumental tasks, is also proposed as valuable for disease monitoring, evaluating potential risk factors and interventions. The reliability of Deacon’s 5-point ordinal scale to score nests at 24 h is well-recognized. However, previous work with the 3xTg-AD mice model of Alzheimer’s disease proposed a 3-day protocol to discard false negatives, thus unveiling genotype-, sex- and age-dependent differences. Here, we propose the size of nesting as a numeric variable, complementary to the ordinal scale, to allow parametric repeated measures analysis for identifying and evaluating temporal patterns in the nest-building process. Thus, nests of male and female mice with normal and AD-pathological aging ‘measured’ during 3-days showed that the nest-building process responded to a linear equation in wild-type animals or when female sex was considered but disrupted in males or the AD-genotype. Genotype per sex interaction indicated the optimal nest-building process in wild-type females, as they build the best nests at 72 h and the worst nests in 3xTg-AD mice at 48 h. On each day, data were consistent with the ordinal scale, but the identification of temporal patterns with the numeric variable confirmed nest-building as a complex process, which is sensitive to sex and genotype. 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