9fc3d5fac127f98aca795f319044db2f pmc_33440672.pdf 5619ba3c49ec66a6ddcbd3317812200aa651a137 pmc_33440672.pdf 4bddc6aa5cdf6d1e9d517aa496b6236505d24098a43fd70e9ad15a0791828581 pmc_33440672.pdf Title: Glial Response and Neuroinflammation in Cerebrocortical Atrophy in a Young Irish Wolfhound Dog Subject: A two-year-old, Irish Wolfhound dog presented with a history of progressive neurological signs. Neurological exam revealed disorientation, absence of menace response, reduction of right nasal sensation, hypermetria and ataxia with reduction of proprioception in all four limbs. MRI findings were compatible with laminar neuronal necrosis and possible bilateral cortical cerebral atrophy. Grossly, a severe bilateral reduction of the gray matter with flattening of gyri, mainly in frontal and parietal cerebral areas, was observed. Histologically, multiple, segmental, bilateral, and symmetric areas of neuronal loss, necrosis and degeneration, in a laminar pattern, associated with a reactive gliosis were observed. Immunohistochemical studies showed severe reduction of neuronal bodies, proliferation and hypertrophy of astrocytes and microglia. Few perivascular B and T cells were demonstrated. Based on these data, we show some of the neuroinflammatory events that occur during CNS repair in a chronic phase of this condition. Keywords: cerebral cortical atrophy; immunohistochemistry; neuronal necrosis; neuropathology Author: Fabiano J. F. de Sant’Ana, Miguel Omaña, Ester Blasco and Martí Pumarola Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jan 11 09:40:26 2021 CET ModDate: Mon Jan 11 09:42:46 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1364522 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PSUYZB+VnURWPalladioL Type 1 Custom yes yes no 10 0 IARHKD+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 INUGMD+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 WETCPX+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 WLENKM+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 SZMRXJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 QGUKZH+CMSY10 Type 1 Builtin yes yes no 78 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 82 0 NHLEGE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 92 0 NHLEHF+PalatinoLinotype TrueType WinAnsi yes yes no 95 0 NHLEHH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 98 0 NHLEGE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 108 0 NHLEHF+PalatinoLinotype TrueType WinAnsi yes yes no 111 0 NHLEHH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 114 0 NHLEGE+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 125 0 NHLEHF+PalatinoLinotype TrueType WinAnsi yes yes no 128 0 NHLEHH+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 131 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 03:53:45 CEST RepresentationInformation: pmc_33440672.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-02-01 03:47:10 CET Size: 1364522 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 208 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Description of the Case Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: Glial Response and Neuroinflammation in Cerebrocortical Atrophy in a Young Irish Wolfhound Dog Author: Fabiano J. F. de Sant’Ana, Miguel Omaña, Ester Blasco and Martí Pumarola Subject: A two-year-old, Irish Wolfhound dog presented with a history of progressive neurological signs. Neurological exam revealed disorientation, absence of menace response, reduction of right nasal sensation, hypermetria and ataxia with reduction of proprioception in all four limbs. MRI findings were compatible with laminar neuronal necrosis and possible bilateral cortical cerebral atrophy. Grossly, a severe bilateral reduction of the gray matter with flattening of gyri, mainly in frontal and parietal cerebral areas, was observed. Histologically, multiple, segmental, bilateral, and symmetric areas of neuronal loss, necrosis and degeneration, in a laminar pattern, associated with a reactive gliosis were observed. Immunohistochemical studies showed severe reduction of neuronal bodies, proliferation and hypertrophy of astrocytes and microglia. Few perivascular B and T cells were demonstrated. Based on these data, we show some of the neuroinflammatory events that occur during CNS repair in a chronic phase of this condition. Keywords: cerebral cortical atrophy; immunohistochemistry; neuronal necrosis; neuropathology Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Jan 11 09:40:26 CET 2021 ModDate: Mon Jan 11 09:42:46 CET 2021 ID: 0x33bc9898b8ae4c653f036d4adafee747, 0x33bc9898b8ae4c653f036d4adafee747 Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: OVNEXW+EURM10 FontSubset: true FirstChar: 22 LastChar: 22 FontDescriptor: FontName: OVNEXW+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: INUGMD+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 241 FontDescriptor: FontName: INUGMD+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WETCPX+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 241 FontDescriptor: FontName: WETCPX+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: PSUYZB+VnURWPalladioL FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: PSUYZB+VnURWPalladioL Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: WLENKM+VnURWPalladioL-Bold FontSubset: true FirstChar: 189 LastChar: 189 FontDescriptor: FontName: WLENKM+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QGUKZH+CMSY10 FontSubset: true FirstChar: 14 LastChar: 14 FontDescriptor: FontName: QGUKZH+CMSY10 Flags: Symbolic FontBBox: -29, -960, 1116, 775 FontFile: true Font: BaseFont: IARHKD+URWPalladioL-Ital FontSubset: true FirstChar: 44 LastChar: 121 FontDescriptor: FontName: IARHKD+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: Differences: true Pages: Page: Sequence: 1 Annotations: Annotation: Subtype: Link Rect: 35, 759, 165, 795 Annotation: Subtype: Link Rect: 515, 759, 560, 795 Annotation: Subtype: Link Rect: 161, 659, 172, 673 Annotation: Subtype: Link Rect: 424, 659, 435, 673 Annotation: Subtype: Link Rect: 63, 313, 154, 322 Annotation: Subtype: Link Rect: 35, 303, 74, 310 Annotation: Subtype: Link Rect: 36, 162, 98, 184 Annotation: Subtype: Link Rect: 123, 89, 154, 98 Annotation: Subtype: Link Rect: 35, 77, 154, 86 Annotation: Subtype: Link Rect: 35, 66, 49, 74 Annotation: Subtype: Link Rect: 257, 131, 264, 140 ActionDest: 138 Annotation: Subtype: Link Rect: 213, 106, 220, 115 ActionDest: 138 Annotation: Subtype: Link Rect: 112, 17, 247, 28 Annotation: Subtype: Link Rect: 407, 17, 558, 28 Annotation: Subtype: Link Rect: 35, 389, 87, 408 Page: Sequence: 2 Annotations: Annotation: Subtype: Link Rect: 477, 737, 484, 746 ActionDest: 138 Annotation: Subtype: Link Rect: 320, 712, 327, 721 ActionDest: 138 Annotation: Subtype: Link Rect: 327, 699, 334, 708 ActionDest: 138 Annotation: Subtype: Link Rect: 281, 661, 288, 670 ActionDest: 138 Annotation: Subtype: Link Rect: 469, 661, 476, 670 ActionDest: 138 Annotation: Subtype: Link Rect: 548, 661, 555, 670 ActionDest: 138 Annotation: Subtype: Link Rect: 397, 649, 404, 658 ActionDest: 138 Annotation: Subtype: Link Rect: 314, 382, 321, 394 ActionDest: 86 Annotation: Subtype: Link Rect: 449, 106, 456, 118 ActionDest: 86 Page: Sequence: 3 Annotations: Annotation: Subtype: Link Rect: 466, 186, 473, 198 ActionDest: 102 Page: Sequence: 4 Annotations: Annotation: Subtype: Link Rect: 436, 320, 443, 332 ActionDest: 119 Annotation: Subtype: Link Rect: 287, 182, 294, 194 ActionDest: 119 Annotation: Subtype: Link Rect: 245, 131, 252, 143 ActionDest: 119 Annotation: Subtype: Link Rect: 452, 119, 459, 131 ActionDest: 119 Page: Sequence: 5 Annotations: Annotation: Subtype: Link Rect: 319, 277, 326, 286 ActionDest: 138 Annotation: Subtype: Link Rect: 477, 202, 484, 211 ActionDest: 138 Annotation: Subtype: Link Rect: 487, 202, 499, 211 ActionDest: 138 Annotation: Subtype: Link Rect: 238, 164, 245, 173 ActionDest: 138 Annotation: Subtype: Link Rect: 505, 126, 512, 135 ActionDest: 138 Annotation: Subtype: Link Rect: 286, 101, 293, 110 ActionDest: 138 Page: Sequence: 6 Annotations: Annotation: Subtype: Link Rect: 255, 584, 267, 593 ActionDest: 138 Annotation: Subtype: Link Rect: 268, 584, 279, 593 ActionDest: 138 Annotation: Subtype: Link Rect: 261, 559, 268, 568 ActionDest: 138 Annotation: Subtype: Link Rect: 368, 547, 375, 556 ActionDest: 138 Page: Sequence: 7 Annotations: Annotation: Subtype: Link Rect: 262, 661, 299, 672 Annotation: Subtype: Link Rect: 306, 661, 342, 672 Annotation: Subtype: Link Rect: 59, 639, 97, 649 Annotation: Subtype: Link Rect: 103, 639, 139, 649 Annotation: Subtype: Link Rect: 127, 616, 164, 626 Annotation: Subtype: Link Rect: 171, 616, 207, 626 Annotation: Subtype: Link Rect: 273, 580, 310, 591 Annotation: Subtype: Link Rect: 316, 580, 352, 591 Annotation: Subtype: Link Rect: 195, 547, 233, 557 Annotation: Subtype: Link Rect: 239, 547, 275, 557 Annotation: Subtype: Link Rect: 519, 523, 557, 534 Annotation: Subtype: Link Rect: 59, 512, 95, 522 Annotation: Subtype: Link Rect: 56, 488, 302, 499 Annotation: Subtype: Link Rect: 59, 455, 97, 465 Annotation: Subtype: Link Rect: 103, 455, 139, 465 Annotation: Subtype: Link Rect: 283, 419, 321, 430 Annotation: Subtype: Link Rect: 327, 419, 363, 430 Annotation: Subtype: Link Rect: 181, 397, 218, 407 Annotation: Subtype: Link Rect: 224, 397, 260, 407 Annotation: Subtype: Link Rect: 131, 351, 168, 361 Annotation: Subtype: Link Rect: 175, 351, 211, 361 Checksum: 08cf8c67 Type: CRC32 Checksum: 9fc3d5fac127f98aca795f319044db2f Type: MD5 Checksum: 5619ba3c49ec66a6ddcbd3317812200aa651a137 Type: SHA-1