7820a3c5851c2be95b9acf7b0891de9b pmc_33634970.pdf 47e08677b8a216e6800bfd4dae5cac48f0cd6e09 pmc_33634970.pdf 8a9df51e623896d3d1d6170bcc3ce792e9da3a8554e3d77d713dce07d63bc316 pmc_33634970.pdf Title: Enforced sialyl‐Lewis‐X (sLeX) display in E‐selectin ligands by exofucosylation is dispensable for CD19‐CAR T‐cell activity and bone marrow homing Subject: Keywords: Author: Creator: LaTeX with hyperref package Producer: Acrobat Distiller 10.1.10 (Windows) CreationDate: Mon Feb 22 18:35:47 2021 CET ModDate: Mon Feb 22 18:36:29 2021 CET Custom Metadata: yes Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 2330014 bytes Optimized: yes PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- MABHFL+STIXTwoText Type 1C Custom yes yes yes 333 0 MABHMJ+STIXTwoText-Bold Type 1C Custom yes yes yes 338 0 MABJOM+STIXTwoText-Italic Type 1C Custom yes yes yes 343 0 MACBOJ+STIXTwoMath Type 1C Custom yes yes yes 131 0 MACCBJ+STIXTwoText-BoldItalic Type 1C WinAnsi yes yes no 136 0 MADBND+STIX-Regular Type 1C Custom yes yes yes 74 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:29:40 CEST RepresentationInformation: pmc_33634970.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:08:59 CEST Size: 2330014 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf Profile: Linearized PDF PDFMetadata: Objects: 400 FreeObjects: 1 IncrementalUpdates: 1 DocumentCatalog: PageLayout: SinglePage PageMode: UseOutlines Outlines: Item: Title: Enforced sialyl-Lewis-X (sLeX) display in E-selectin ligands by exofucosylation is dispensable for CD19-CAR T-cell activity and bone marrow homing Destination: chapter..1 Children: Item: Title: Abstract Destination: section*.2 Item: Title: 1 | INTRODUCTION Destination: section..1.1 Item: Title: 2 | RESULTS Destination: section..1.2 Children: Item: Title: 2.1 | Cell-autonomous and exofucosylation-enforced expression of sLeX in CD19-CAR T cells Destination: subsection..1.2.1 Item: Title: 2.2 | Exofucosylation enhances neither cytotoxic activity nor homing of CAR T cells in vitro Destination: subsection..1.2.2 Item: Title: 2.3 | Exofucosylation enhances neither homing to BM/spleen nor activity/persistence of CAR T cells in vivo Destination: subsection..1.2.3 Item: Title: 3 | DISCUSSION Destination: section..1.3 Item: Title: 4 | MATERIALS AND METHODS Destination: section..1.4 Children: Item: Title: 4.1 | CD19-CAR vector, lentiviral production, T-cell transduction, activation and expansion Destination: subsection..1.4.1 Item: Title: 4.2 | Exofucosylation reaction Destination: subsection..1.4.2 Item: Title: 4.3 | E-selectin-Ig immunoprecipitation Destination: subsection..1.4.3 Children: Item: Title: 4.3.1 | In vitro cytotoxicity assays Destination: subsubsection..1.4.3.1 Item: Title: 4.4 | HUVEC transwell assays Destination: subsection..1.4.4 Item: Title: 4.5 | In vivo NALM6, SEM, and B-ALL patient-derived xenograft (PDX) models Destination: subsection..1.4.5 Item: Title: 4.6 | FACS analysis Destination: subsection..1.4.6 Item: Title: 4.7 | Statistical analysis Destination: subsection..1.4.7 Item: Title: ACKNOWLEDGMENTS Destination: section*.4 Item: Title: AUTHOR CONTRIBUTIONS Destination: section*.6 Item: Title: CONFLICT OF INTEREST Destination: section*.8 Item: Title: APPROVAL AND CONSENT TO PARTICIPATE Destination: section*.10 Item: Title: AVAILABILITY OF DATA AND MATERIAL Destination: section*.12 Item: Title: ORCID Destination: section*.14 Item: Title: REFERENCES Destination: section*.16 Item: Title: SUPPORTING INFORMATION Destination: section*.18 Info: Title: Enforced sialyl‐Lewis‐X (sLeX) display in E‐selectin ligands by exofucosylation is dispensable for CD19‐CAR T‐cell activity and bone marrow homing Author: 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