5a0260c6cec3f3a92e6606eb5ddb8613 pmc_33652808.pdf caa74e429d30d2f1c0041ef1c5377b972d8e8465 pmc_33652808.pdf cbf90ed979b8fcdb639769e6bbce247e3d2841c5b93809467283d3e36f0393bb pmc_33652808.pdf Title: Cytochrome c Deficiency Differentially Affects the In Vivo Mitochondrial Electron Partitioning and Primary Metabolism Depending on the Photoperiod Subject: Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under short- and long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods. Keywords: alternative oxidase (AOX); cytochrome c (CYTc); oxygen isotope discrimination; metabolite profiling; photoperiod; primary metabolism Author: Igor Florez-Sarasa, Elina Welchen, Sofia Racca, Daniel H. Gonzalez, José G. Vallarino, Alisdair R. Fernie, Miquel Ribas-Carbo and Nestor Fernandez Del-Saz Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Mar 11 10:12:50 2021 CET ModDate: Fri Mar 12 03:21:34 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 18 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1704257 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- BTFGED+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 YWQWLZ+VnURWPalladioL Type 1 Custom yes yes no 15 0 VWWNCO+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 CQVZDQ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 UHQFRO+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 ABFWDH+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 VTLKDP+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 41 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 71 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 75 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 79 0 QMDDXF+CMSY10 Type 1 Builtin yes yes no 100 0 YZZPJS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 109 0 TZQQRC+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 113 0 OXBHZM+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 117 0 PHAGFY+EURB10 Type 1 Builtin yes yes no 126 0 QQOGDI+EURM10 Type 1 Builtin yes yes no 130 0 DWOYBI+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 147 0 YZZPJS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 155 0 TZQQRC+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 159 0 OXBHZM+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 163 0 YZZPJS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 182 0 TZQQRC+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 186 0 OXBHZM+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 190 0 YZZPJS+PalatinoLinotype-Italic TrueType WinAnsi yes yes yes 205 0 TZQQRC+PalatinoLinotype-Bold TrueType WinAnsi yes yes yes 209 0 OXBHZM+PalatinoLinotype-Roman TrueType WinAnsi yes yes yes 213 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:29:13 CEST RepresentationInformation: pmc_33652808.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:02 CEST Size: 1704257 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 567 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Differences in the Relative Expression of Respiratory Chain Components between Cytc Mutants and WT Plants under Short- and Long-Day Photoperiods Destination: subsection.2.1 Item: Title: Respiration, Electron Partitioning to the AOX Pathway, and Photosynthesis in Cytc Mutants and WT Plants under Short- and Long-Day Photoperiods Destination: subsection.2.2 Item: Title: Metabolite Profiling in Cytc Mutants and WT Plants under Short- and Long-Day Photoperiods Destination: subsection.2.3 Item: Title: Discussion Destination: section.3 Item: Title: Conclusions Destination: section.4 Item: Title: Materials and Methods Destination: section.5 Children: Item: Title: Plant Material and Growth Conditions Destination: subsection.5.1 Item: Title: Protein and Transcript Levels Destination: subsection.5.2 Item: Title: Respiration and Oxygen Isotope Discrimination Measurements Destination: subsection.5.3 Item: Title: Leaf Gas Exchange and Chlorophyll Fluorescence Measurements Destination: subsection.5.4 Item: Title: Metabolite Profiling Destination: subsection.5.5 Item: Title: Statistical Analyses Destination: subsection.5.6 Item: Title: References Destination: subsection.5.6 Info: Title: Cytochrome c Deficiency Differentially Affects the In Vivo Mitochondrial Electron Partitioning and Primary Metabolism Depending on the Photoperiod Author: Igor Florez-Sarasa, Elina Welchen, Sofia Racca, Daniel H. Gonzalez, José G. Vallarino, Alisdair R. Fernie, Miquel Ribas-Carbo and Nestor Fernandez Del-Saz Subject: Plant respiration provides metabolic flexibility under changing environmental conditions by modulating the activity of the nonphosphorylating alternative pathways from the mitochondrial electron transport chain, which bypass the main energy-producing components of the cytochrome oxidase pathway (COP). While adjustments in leaf primary metabolism induced by changes in day length are well studied, possible differences in the in vivo contribution of the COP and the alternative oxidase pathway (AOP) between different photoperiods remain unknown. In our study, in vivo electron partitioning between AOP and COP and expression analysis of respiratory components, photosynthesis, and the levels of primary metabolites were studied in leaves of wild-type (WT) plants and cytochrome c (CYTc) mutants, with reduced levels of COP components, under short- and long-day photoperiods. Our results clearly show that differences in AOP and COP in vivo activities between WT and cytc mutants depend on the photoperiod likely due to energy and stress signaling constraints. Parallel responses observed between in vivo respiratory activities, TCA cycle intermediates, amino acids, and stress signaling metabolites indicate the coordination of different pathways of primary metabolism to support growth adaptation under different photoperiods. Keywords: alternative oxidase (AOX); cytochrome c (CYTc); oxygen isotope discrimination; metabolite profiling; photoperiod; primary metabolism Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Mar 11 10:12:50 CET 2021 ModDate: Fri Mar 12 03:21:34 CET 2021 ID: 0x9028ba535b73db30426ae288bd049b4c, 0x9028ba535b73db30426ae288bd049b4c Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: ABFWDH+URWPalladioL-Roma FontSubset: true FirstChar: 2 LastChar: 252 FontDescriptor: FontName: ABFWDH+URWPalladioL-Roma Flags: Symbolic FontBBox: -166, -283, 1021, 943 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: QQOGDI+EURM10 FontSubset: true FirstChar: 22 LastChar: 28 FontDescriptor: FontName: QQOGDI+EURM10 Flags: Symbolic FontBBox: -32, -243, 1060, 720 FontFile: true Font: BaseFont: VWWNCO+URWPalladioL-Ital FontSubset: true FirstChar: 2 LastChar: 121 FontDescriptor: FontName: VWWNCO+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 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Annotation: Subtype: Link Rect: 278, 629, 316, 640 Annotation: Subtype: Link Rect: 322, 629, 358, 640 Annotation: Subtype: Link Rect: 416, 606, 454, 617 Annotation: Subtype: Link Rect: 325, 583, 363, 594 Annotation: Subtype: Link Rect: 400, 560, 437, 571 Annotation: Subtype: Link Rect: 444, 560, 480, 571 Annotation: Subtype: Link Rect: 157, 538, 195, 548 Annotation: Subtype: Link Rect: 104, 503, 141, 513 Annotation: Subtype: Link Rect: 297, 479, 334, 490 Annotation: Subtype: Link Rect: 340, 479, 376, 490 Annotation: Subtype: Link Rect: 91, 457, 128, 467 Annotation: Subtype: Link Rect: 104, 434, 142, 444 Annotation: Subtype: Link Rect: 148, 434, 184, 444 Annotation: Subtype: Link Rect: 520, 410, 557, 421 Annotation: Subtype: Link Rect: 363, 387, 401, 398 Annotation: Subtype: Link Rect: 407, 387, 443, 398 Annotation: Subtype: Link Rect: 301, 364, 338, 375 Annotation: Subtype: Link Rect: 131, 331, 169, 341 Annotation: Subtype: Link Rect: 507, 307, 545, 318 Annotation: Subtype: Link Rect: 155, 285, 192, 295 Annotation: Subtype: Link Rect: 402, 261, 439, 272 Annotation: Subtype: Link Rect: 263, 238, 301, 249 Annotation: Subtype: Link Rect: 296, 226, 334, 237 Annotation: Subtype: Link Rect: 465, 215, 501, 226 Annotation: Subtype: Link Rect: 377, 192, 415, 203 Annotation: Subtype: Link Rect: 421, 192, 457, 203 Annotation: Subtype: Link Rect: 462, 169, 499, 180 Annotation: Subtype: Link Rect: 505, 169, 541, 180 Annotation: Subtype: Link Rect: 284, 146, 321, 157 Annotation: Subtype: Link Rect: 327, 146, 363, 157 Annotation: Subtype: Link Rect: 191, 112, 229, 122 Annotation: Subtype: Link Rect: 203, 88, 241, 99 Annotation: Subtype: Link Rect: 439, 65, 477, 76 Annotation: Subtype: Link Rect: 483, 65, 519, 76 Page: Sequence: 18 Annotations: Annotation: Subtype: Link Rect: 417, 721, 454, 732 Annotation: Subtype: Link Rect: 461, 721, 497, 732 Annotation: Subtype: Link Rect: 330, 698, 368, 709 Annotation: Subtype: Link Rect: 374, 698, 410, 709 Checksum: 5753142b Type: CRC32 Checksum: 5a0260c6cec3f3a92e6606eb5ddb8613 Type: MD5 Checksum: caa74e429d30d2f1c0041ef1c5377b972d8e8465 Type: SHA-1 Checksum: cbf90ed979b8fcdb639769e6bbce247e3d2841c5b93809467283d3e36f0393bb Type: SHA-256