34cc66f49cdc996acdae574b21b1e1bf pmc_33731752.pdf 04bae5ef3a217cf5d1e5cd570df525e63685fa07 pmc_33731752.pdf 65ac9fe3910e76ae38be0d25c6eec88213352a28d0c6752539c0a882962930d1 pmc_33731752.pdf Title: Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning Subject: Scientific Reports, https://doi.org/10.1038/s41598-021-85460-7 Author: M. Saladrigas-García Creator: Springer Producer: Adobe PDF Library 15.0; modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Thu Mar 11 03:35:32 2021 CET ModDate: Thu Mar 11 08:23:09 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 13 Encrypted: no Page size: 595.276 x 782.362 pts Page rot: 0 File size: 1656516 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VIBELH+Wingdings-Regular Type 1C WinAnsi yes yes yes 380 0 MinionPro-It Type 1C Custom yes no yes 42 0 MinionPro-Regular Type 1C Custom yes no yes 41 0 ILSJQH+Corbel-Bold CID TrueType Identity-H yes yes yes 43 0 Corbel2 TrueType WinAnsi yes no yes 44 0 CHUEKT+Corbel CID TrueType Identity-H yes yes yes 45 0 CHUEKT+Corbel CID TrueType Identity-H yes yes yes 413 0 MinionPro-Bold Type 1C Custom yes no yes 68 0 WYOVDF+MinionPro-BoldIt Type 1C WinAnsi yes yes yes 79 0 Corbel2 TrueType WinAnsi yes no yes 342 0 ZQRRPZ+MTSYN4 Type 1C Custom yes yes no 93 0 AUGXDX+MinionPro-Regular3 Type 1C Custom yes yes no 94 0 AUGXDX+MinionPro-Regular2 Type 1C Custom yes yes no 95 0 ZQRRPZ+MTSYN3 Type 1C Custom yes yes no 96 0 AUGXDX+MinionPro-It2 Type 1C Custom yes yes no 97 0 AUGXDX+MinionPro-Regular4 Type 1C Custom yes yes no 98 0 ZQRRPZ+MTSYN2 Type 1C Custom yes yes no 99 0 ZQRRPZ+MTSYN Type 1C Custom yes yes no 100 0 ZQRRPZ+MTSYN5 Type 1C Custom yes yes no 101 0 YRCLBB+MTMI Type 1C Custom yes yes no 102 0 YRCLBB+MTMI Type 1C Custom yes yes no 103 0 ZQRRPZ+MTSYN7 Type 1C Custom yes yes no 104 0 AUGXDX+MinionPro-Regular5 Type 1C Custom yes yes no 105 0 ZQRRPZ+MTSYN6 Type 1C Custom yes yes no 106 0 WODZZF+TimesNewRomanPSMT TrueType WinAnsi yes yes yes 303 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 02:48:11 CEST RepresentationInformation: pmc_33731752.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:09:12 CEST Size: 1656516 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 463 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: false CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en-US Outlines: Item: Title: Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning Children: Item: Title: Results Children: Item: Title: Caecal microbiota (16S rRNA gene sequencing). Children: Item: Title: Microbiota structure and biodiversity. Item: Title: Taxonomy of caecal microbiota. Item: Title: Jejunal gene expression. Item: Title: Metabolomic response. Item: Title: Integration of the omics technologies. Item: Title: Functionality of the large intestine. Item: Title: Discussion Item: Title: Conclusion Item: Title: Methods Children: Item: Title: Animals and study design. Item: Title: Blood and intestinal sampling. Item: Title: DNA extraction and 16S rRNA gene sequencing. Item: Title: Sequencing data bioinformatics. Item: Title: RNA extraction and cDNA preparation. Item: Title: Plate design and gene expression study by qPCR. Item: Title: Nuclear magnetic resonance spectroscopy. Item: Title: 1H-NMR data pre-processing. Item: Title: Ussing chamber experiments. Item: Title: Statistical analysis. Item: Title: References Item: Title: Acknowledgements Info: Title: Early socialization and environmental enrichment of lactating piglets affects the caecal microbiota and metabolomic response after weaning Author: M. 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