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Title:           Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardiac scaffolds for myocardial infarction
Subject:         Bioactive Materials, 6 (2021) 3314-3327. doi:10.1016/j.bioactmat.2021.02.026
Keywords:        Exosomes,Mesenchymal stem/stromal cells,Migration,Infiltration,Cardiac tissue engineering
Author:          Marta Monguió-Tortajada
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      Title: Local administration of porcine immunomodulatory, chemotactic and angiogenic extracellular vesicles using engineered cardia ...
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        Title: 1 Introduction
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        Title: 2 Materials and methods
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          Title: 2.1 Porcine cardiac adipose tissue-derived MSC isolation and culture
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          Title: 2.2 Extracellular vesicle production and isolation
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          Title: 2.3 Bead-based flow cytometry
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          Title: 2.4 Cryo-electron microscopy
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          Title: 2.5 Nanoparticle tracking analysis
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          Title: 2.6 EV staining
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          Title: 2.7 Endotoxin detection
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          Title: 2.8 Porcine peripheral blood mononuclear cells isolation and functional assays
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          Title: 2.9 Cytokine response
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          Title: 2.10 Porcine outgrowing endothelial progenitor cell cultures and characterization
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          Title: 2.11 Migration assay
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          Title: 2.12 Tube formation assay
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          Title: 2.13 Generation of cardiac scaffolds
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          Title: 2.14 EV delivery into decellularised scaffolds
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          Title: 2.15 Near-infrared scaffold scanning
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          Title: 2.16 Scanning electron microscopy
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          Title: 2.17 Myocardial infarction pig model
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          Title: 2.18 Tissue collection, morphometric and histopathological analyses
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          Title: 2.19 Immunohistochemical analysis
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          Title: 2.20 Statistical analysis
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        Title: 3 Results
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          Title: 3.1 Porcine cATMSC-EV purification
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          Title: 3.2 cATMSC-EV have immune suppressive properties
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          Title: 3.3 Porcine cATMSC-EV recruit allogeneic pro-regenerative cells and promote angiogenesis
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          Title: 3.4 Biocompatible 3D-Engineered scaffolds maintain the internal ultrastructure and are devoid of cells after decellularisation
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          Title: 3.5 Porcine cATMSC-EV are successfully retained within 3D-Engineered cardiac scaffolds
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          Title: 3.6 Porcine cATMSC-EV combined with cardiac scaffolds abrogate the cytokine response of allogeneic PBMC
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          Title: 3.7 Locally administered porcine cATMSC-EV stay within scaffolds and infarct core
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          Title: 3.8 In vivo scaffold neovascularization and reduction of macrophage and T cell infiltration
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        Title: 4 Discussion
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        Title: Funding sources
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        Title: CRediT authorship contribution statement
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        Title: Declaration of competing interest
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        Title: Acknowledgements
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        Title: Abbreviations
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        Title: Appendix A Supplementary data
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        Title: References
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    Author: Marta Monguió-Tortajada
    Subject: Bioactive Materials, 6 (2021) 3314-3327. doi:10.1016/j.bioactmat.2021.02.026
    Keywords: Exosomes,Mesenchymal stem/stromal cells,Migration,Infiltration,Cardiac tissue engineering
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  Checksum: 6179ed1b
   Type: CRC32
  Checksum: c0537509cd66e60e7b057dc9737e45a7
   Type: MD5
  Checksum: ef82ed73631c34e889e7bad07593b6e8205024fe
   Type: SHA-1
  Checksum: bde8e6f2730ef4019c7243a0202b9ca388688797d943f9fccd07e0589e28c76d
   Type: SHA-256