c4fb1387e2b8413a6d7e6777b891f753 pmc_33799577.pdf 034a7e3a7db2abc8b26611f04d25077c1211e3cd pmc_33799577.pdf 93880df4df41fa156232b7c7b8aea265ca4383738df5afd95d3d3c42e91fdb0a pmc_33799577.pdf Title: Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats Subject: Cats are the primary reservoir host for Bartonella henselae (B. henselae), an etiological agent of human bartonellosis, including cat scratch disease. Although Bartonella DNA has been amplified from salivary swabs from cats, dogs and humans, we are not aware of studies investigating Bartonella antibodies in oral fluid (OF). Using inhouse and commercial immunofluorescence antibody assays (IFA), the objective of this study was to detect and compare antibodies against B. henselae in paired OF and serum specimens from cats. Specimens were collected from shelter and client-owned cats. For serum specimens, B. henselae seroreactivity was 78% for both the inhouse and commercial IFA assays and 56.8% for OF specimens. Comparing serum and OF specimens, there was moderate Kappa agreement (Cohen’s k = 0.434) for detection of B. henselae antibodies. Oral fluid antibodies were more likely measurable in cats with high B. henselae serum antibody titers when compared with low antibody titers. In conclusion, B. henselae OF IFA antibody measurements were less sensitive compared to serum IFA measurements of 1:64. Oral fluid antibodies were detected more often in cats with high B. henselae serum antibody titers. Therefore, OF antibodies, detectable by IFA, is of limited utility for epidemiological or diagnostic testing in cats. Keywords: bartonellosis; feline; serology; immunofluorescence antibody assay; oral fluid Author: Alejandra Álvarez-Fernández, Marta Baxarias, David Prandi, Edward B. Breitschwerdt and Laia Solano-Gallego Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 16 04:50:51 2021 CET ModDate: Tue Mar 16 05:12:19 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 5705188 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- RAFMMA+VnURWPalladioL Type 1 Custom yes yes no 10 0 YTKFOJ+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 RWXCXH+URWPalladioL-BoldItal Type 1 Custom yes yes no 20 0 YRIBLO+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 AWOXQL+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 KXDJCR+CMSY10 Type 1 Builtin yes yes no 32 0 CBKAWJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 36 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 45 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 67 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 71 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 75 0 AYXMDM+EURM10 Type 1 Builtin yes yes no 90 0 RSPJNP+EURB10 Type 1 Builtin yes yes no 94 0 GIHOMG+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 111 0 GIIFIH+TimesNewRoman,BoldItalic TrueType WinAnsi yes yes no 117 0 GIHONH+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 120 0 GIHONI+PalatinoLinotype CID TrueType Identity-H yes yes yes 123 0 GIHONJ+PalatinoLinotype TrueType WinAnsi yes yes no 129 0 GIHONL+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 132 0 GIIFIF+TimesNewRoman,Bold CID TrueType Identity-H yes yes yes 135 0 LJCKMA+PalatinoLinotype,Bold CID TrueType Identity-H yes yes yes 154 0 LJCKNB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 160 0 LJCKND+PalatinoLinotype TrueType WinAnsi yes yes no 163 0 LJCKOE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 166 0 LJCLNB+PalatinoLinotype,BoldItalic TrueType WinAnsi yes yes no 169 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:25:53 CEST RepresentationInformation: pmc_33799577.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:21 CEST Size: 5705188 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 356 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Children: Item: Title: Description of the Study Cats Destination: subsection.2.1 Item: Title: Inhouse Versus Commercial IFA Testing of Cat Sera Destination: subsection.2.2 Item: Title: Commercial IFA in Serum and OF Samples Destination: subsection.2.3 Item: Title: Modified Commercial IFA Testing of OF Specimens Destination: subsection.2.4 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Children: Item: Title: Cats Destination: subsection.4.1 Item: Title: Serum Specimens Destination: subsection.4.2 Item: Title: Oral Fluid Specimens Destination: subsection.4.3 Item: Title: Bartonella henselae Inhouse IFA Serological Testing Destination: subsection.4.4 Item: Title: Bartonella henselae Commercial IFA Paired Serological and OF Testing Destination: subsection.4.5 Item: Title: Modified B. henselae Commercial IFA OF Testing Destination: subsection.4.6 Item: Title: Statistical Analysis Destination: subsection.4.7 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Bartonella henselae Antibodies in Serum and Oral Fluid Specimens from Cats Author: Alejandra Álvarez-Fernández, Marta Baxarias, David Prandi, Edward B. Breitschwerdt and Laia Solano-Gallego Subject: Cats are the primary reservoir host for Bartonella henselae (B. henselae), an etiological agent of human bartonellosis, including cat scratch disease. Although Bartonella DNA has been amplified from salivary swabs from cats, dogs and humans, we are not aware of studies investigating Bartonella antibodies in oral fluid (OF). Using inhouse and commercial immunofluorescence antibody assays (IFA), the objective of this study was to detect and compare antibodies against B. henselae in paired OF and serum specimens from cats. Specimens were collected from shelter and client-owned cats. For serum specimens, B. henselae seroreactivity was 78% for both the inhouse and commercial IFA assays and 56.8% for OF specimens. Comparing serum and OF specimens, there was moderate Kappa agreement (Cohen’s k = 0.434) for detection of B. henselae antibodies. Oral fluid antibodies were more likely measurable in cats with high B. henselae serum antibody titers when compared with low antibody titers. In conclusion, B. henselae OF IFA antibody measurements were less sensitive compared to serum IFA measurements of 1:64. Oral fluid antibodies were detected more often in cats with high B. henselae serum antibody titers. Therefore, OF antibodies, detectable by IFA, is of limited utility for epidemiological or diagnostic testing in cats. 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