78c168585cebdc8b8083cd8a63e9b24d pmc_33800211.pdf 22d6e0dbeab369950c8be051cd61f4ba614d56b5 pmc_33800211.pdf 5c324d3252df089afe9d9df6c14b025daafe1bd933248bb404c9313a03ff628d pmc_33800211.pdf Title: Utility of the Cobas® Plasma Separation Card as a Sample Collection Device for Serological and Virological Diagnosis of Hepatitis C Virus Infection Subject: Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests. The aim of this study was to compare the performance of dried plasma spots (DPS), prepared using the cobas® Plasma Separation Card (PSC), to plasma and serum from venipuncture, for HCV diagnosis. We carried out a prospective study using DPS and paired plasma or serum samples. Serum and DPS samples were analyzed by immunoassay using Elecsys® Anti-HCV II (Roche). Plasma and DPS samples were analyzed using the cobas® HCV viral load and cobas® HCV genotyping tests (Roche). All DPS samples that had high anti-HCV antibody titers in serum were also antibody-positive, as were five of eight samples with moderate titers. Eight samples with low titers in serum were negative with DPS. Among 80 samples with plasma HCV viral loads between 61.5 and 2.2 108 IU/mL, 74 were RNA-positive in DPS. The mean viral load difference between plasma and DPS was 2.65 log10 IU/mL. The performance of DPS for detection of serological and virological markers of hepatitis C virus infection was comparable to that of the conventional specimen types. However, the limits of detection were higher for DPS. Keywords: hepatitis C; dried plasma spot; plasma separation card Author: Fernando Velásquez-Orozco, Ariadna Rando-Segura, Joan Martínez-Camprecios, Paula Salmeron, Adrián Najarro-Centeno, Àngels Esteban, Josep Quer, María Buti, Tomás Pumarola-Suñe and Francisco Rodríguez-Frías Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 10 06:10:17 2021 CET ModDate: Wed Mar 10 06:13:45 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1598914 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- TYDNEH+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 YOSCZR+VnURWPalladioL Type 1 Custom yes yes no 15 0 QZXSEE+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 ZTZZXJ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 KNZPDC+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 IAFOCY+CMSY10 Type 1 Builtin yes yes no 32 0 BXZUUH+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 85 0 OJNJCM+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 96 0 OJNJAG+TimesNewRomanPSMT TrueType WinAnsi yes yes no 99 0 OJNJAH+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 102 0 OJNJBI+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 108 0 OJNJCJ+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 111 0 OJNJCK+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 117 0 OJNJCL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 120 0 OJNJCM+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 150 0 OJOKAI+Arial-BoldMT TrueType WinAnsi yes yes no 153 0 OJNJAG+TimesNewRomanPSMT TrueType WinAnsi yes yes no 156 0 OJNJAH+PalatinoLinotype-Italic CID TrueType Identity-H yes yes yes 159 0 OJNJBI+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 165 0 OJNJCJ+PalatinoLinotype-Bold CID TrueType Identity-H yes yes yes 168 0 OJNJCK+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 174 0 OJNJCL+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 177 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 02:47:37 CEST RepresentationInformation: pmc_33800211.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:09:22 CEST Size: 1598914 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 288 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Children: Item: Title: Study Design Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Sample Preparation Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Serological Testing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Viral Load Testing Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: HCV Genotyping Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Children: Item: Title: Serological Diagnosis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Determination of HCV RNA Viral Load Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Determination of HCV Genotype Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Info: Title: Utility of the Cobas® Plasma Separation Card as a Sample Collection Device for Serological and Virological Diagnosis of Hepatitis C Virus Infection Author: Fernando Velásquez-Orozco, Ariadna Rando-Segura, Joan Martínez-Camprecios, Paula Salmeron, Adrián Najarro-Centeno, Àngels Esteban, Josep Quer, María Buti, Tomás Pumarola-Suñe and Francisco Rodríguez-Frías Subject: Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests. The aim of this study was to compare the performance of dried plasma spots (DPS), prepared using the cobas® Plasma Separation Card (PSC), to plasma and serum from venipuncture, for HCV diagnosis. We carried out a prospective study using DPS and paired plasma or serum samples. Serum and DPS samples were analyzed by immunoassay using Elecsys® Anti-HCV II (Roche). Plasma and DPS samples were analyzed using the cobas® HCV viral load and cobas® HCV genotyping tests (Roche). All DPS samples that had high anti-HCV antibody titers in serum were also antibody-positive, as were five of eight samples with moderate titers. Eight samples with low titers in serum were negative with DPS. Among 80 samples with plasma HCV viral loads between 61.5 and 2.2 108 IU/mL, 74 were RNA-positive in DPS. The mean viral load difference between plasma and DPS was 2.65 log10 IU/mL. The performance of DPS for detection of serological and virological markers of hepatitis C virus infection was comparable to that of the conventional specimen types. However, the limits of detection were higher for DPS. 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