b6997a33434655c1e197896b111480a9 pmc_33803363.pdf 3d9ed1c4af9444b78ef063a19cf5a7bc3fa24edc pmc_33803363.pdf c9e6dc134bb929fe799f03b0633ac1a09c156d45a6eb6e6fc87e6dee4b73ce03 pmc_33803363.pdf Title: Two Archaeal Metagenome-Assembled Genomes from El Tatio Provide New Insights into the Crenarchaeota Phylum Subject: A phylogenomic and functional analysis of the first two Crenarchaeota MAGs belonging to El Tatio geysers fields in Chile is reported. A soil sample contiguous to a geothermal activity exposed lagoon of El Tatio was used for shotgun sequencing. Afterwards, contigs were binned into individual population-specific genomes data. A phylogenetic placement was carried out for both MAG 9-5TAT and MAG 47-5TAT. Then functional comparisons and metabolic reconstruction were carried out. Results showed that both MAG 9-5TAT and MAG 47-5TAT likely represent new species in the genus Thermoproteus and the genus Sulfolobus, respectively. These findings provide new insights into the phylogenetic and genomic diversity for archaea species that inhabit the El Tatio geysers field and expand the understanding of the Crenarchaeota phylum diversity. Keywords: extreme environments; hot-springs; phylogenetic placement Author: Andrés Santos, Pablo Bruna, Jaime Martinez-Urtaza, Francisco Solís, Bernardita Valenzuela, Pedro Zamorano and Leticia Barrientos Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 9 14:13:44 2021 CET ModDate: Tue Mar 9 14:16:17 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1540727 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- XPROCP+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 GWPAYG+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 ETBXZM+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 XYPWHM+CMSY10 Type 1 Builtin yes yes no 23 0 YSPQJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 27 0 KONDUX+EuphemiaUCAS-Bold Type 1C WinAnsi yes yes no 36 0 HIZBRM+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 39 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 61 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 65 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 69 0 OPZDHV+VnURWPalladioL Type 1 Custom yes yes no 105 0 CKBPVA+EURM10 Type 1 Builtin yes yes no 112 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:25:09 CEST RepresentationInformation: pmc_33803363.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:29 CEST Size: 1540727 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 257 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Site Information and Sample Collection Destination: subsection.2.1 Item: Title: DNA Extraction and Sequencing. Destination: subsection.2.2 Item: Title: Metagenome-Assembled Genomes Binning Destination: subsection.2.3 Item: Title: Taxonomic and Phylogenetic Inference of MAGs Destination: subsection.2.4 Item: Title: Metabolic Reconstruction and Functional Annotation Destination: subsection.2.5 Item: Title: Pangenomic Comparison Destination: subsection.2.6 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Metagenome-Assembled Genome Binning Destination: subsection.3.1 Item: Title: Phylogenetic Placement of the MAGs Destination: subsection.3.2 Item: Title: Functional Annotation and Metabolic Reconstruction of the MAGs Destination: subsection.3.3 Item: Title: Pangenomic Analysis of the MAGs Destination: subsection.3.4 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Two Archaeal Metagenome-Assembled Genomes from El Tatio Provide New Insights into the Crenarchaeota Phylum Author: Andrés Santos, Pablo Bruna, Jaime Martinez-Urtaza, Francisco Solís, Bernardita Valenzuela, Pedro Zamorano and Leticia Barrientos Subject: A phylogenomic and functional analysis of the first two Crenarchaeota MAGs belonging to El Tatio geysers fields in Chile is reported. A soil sample contiguous to a geothermal activity exposed lagoon of El Tatio was used for shotgun sequencing. Afterwards, contigs were binned into individual population-specific genomes data. A phylogenetic placement was carried out for both MAG 9-5TAT and MAG 47-5TAT. Then functional comparisons and metabolic reconstruction were carried out. Results showed that both MAG 9-5TAT and MAG 47-5TAT likely represent new species in the genus Thermoproteus and the genus Sulfolobus, respectively. These findings provide new insights into the phylogenetic and genomic diversity for archaea species that inhabit the El Tatio geysers field and expand the understanding of the Crenarchaeota phylum diversity. 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