861a8d676925122cade289b2d305f342 pmc_33806840.pdf 178b03d6ad96a41461793d09679bd3313754d9a5 pmc_33806840.pdf 5ba29b5156224e12e0e0dd3251a1f40ef13901aa57fb3c2e70c4f637a7e76f09 pmc_33806840.pdf Title: Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain Subject: Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2–1.3) of the Pyrenean chamois (Rupicapra pyrenaica) and 1.4% (CI95% <0.01–8.1) of the sheep (Ovis aries) sampled, with Chlamydia pecorum the only chlamydial species identified. No association of C. pecorum with ocular disease or co-infection with Mycoplasma conjunctivae was found. Further studies on the pathogenesis of infectious keratoconjunctivitis are needed to better understand the ecology of C. pecorum and its possible role as a ruminant pathogen at the wildlife–livestock interface. Keywords: Chlamydia pecorum; domestic sheep; infectious keratoconjunctivitis; ocular disease; Pyrenean chamois; Rupicapra pyrenaica; wildlife–livestock interface Author: Andrea Dias-Alves, Oscar Cabezón, Nicole Borel, Jorge Ramón López-Olvera, Gregorio Mentaberre, Santiago Lavín and Xavier Fernández Aguilar Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 23 10:49:52 2021 CET ModDate: Tue Mar 23 10:53:53 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 7 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 271989 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRJKGQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 RLYSKC+VnURWPalladioL Type 1 Custom yes yes no 15 0 QVQBGI+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 XEFYJJ+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 MIYDBS+URWPalladioL-BoldItal Type 1 Custom yes yes no 28 0 HPHZOV+URWPalladioL-Roma Type 1 Custom yes yes no 32 0 UXHOAG+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 41 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 63 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 67 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 71 0 AAPCWM+CMSY10 Type 1 Builtin yes yes no 89 0 RFECCF+EURM10 Type 1 Builtin yes yes no 93 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:24:33 CEST RepresentationInformation: pmc_33806840.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:31 CEST Size: 271989 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 232 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Results Destination: section.2 Item: Title: Discussion Destination: section.3 Item: Title: Materials and Methods Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Molecular Detection and Identification of Chlamydiaceae in the Eyes of Wild and Domestic Ruminant Hosts from Northern Spain Author: Andrea Dias-Alves, Oscar Cabezón, Nicole Borel, Jorge Ramón López-Olvera, Gregorio Mentaberre, Santiago Lavín and Xavier Fernández Aguilar Subject: Infections by Chlamydiae are associated with ocular disease in humans and animals. In this study, the presence and diversity of Chlamydia spp. was assessed in diseased and healthy eyes of domestic sheep and wild ruminants that share mountain habitats in northern Spain. The presence of Chlamydia spp. was tested by real-time PCR in 1786 conjunctival swabs collected from both eyes of 893 animals from mountain habitats in northern Spain, and chlamydial species were identified in the positive samples by ArrayTube microarray methods. Chlamydial DNA was detected in 0.6% (CI95% 0.2 1.3) of the Pyrenean chamois (Rupicapra pyrenaica) and 1.4% (CI95% <0.01 8.1) of the sheep (Ovis aries) sampled, with Chlamydia pecorum the only chlamydial species identified. No association of C. pecorum with ocular disease or co-infection with Mycoplasma conjunctivae was found. Further studies on the pathogenesis of infectious keratoconjunctivitis are needed to better understand the ecology of C. pecorum and its possible role as a ruminant pathogen at the wildlife livestock interface. 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