e3af4dee52474135e6e57cfeabee3c57 pmc_33809899.pdf 9a70df7e60e98fe4dada0f20b2aaa493cdc9b671 pmc_33809899.pdf 08c90788be8c6c645fcfbd38269cdc6efe123b0ed5bf88ee661c15665ea77af9 pmc_33809899.pdf Title: Effects of L-Glutamine Supplementation during the Gestation of Gilts and Sows on the Offspring Development in a Traditional Swine Breed Subject: The use of amino acids during pregnancy, such as glutamine (Gln), seems to be a promising strategy in selected swine breeds to improve the offspring prenatal development. The main goal of the current study was to assess the development of the offspring from parity 1–3 sows of a traditional breed, which were supplemented with 1% glutamine after Day 35 of gestation, under farm conditions. A total of 486 (288 treated) piglets from 78 (46 treated) Iberian sows were used. At birth and slaughterhouse, fatty acid composition, metabolism, and mTOR pathway gene expression were analyzed. At birth, treated newborns showed greater amounts of specific amino acids in plasma, such as glutamine, asparagine, or alanine, and n-3 fatty acids in cellular membranes than control newborns. The expression of genes belonging to mTOR Complex 1 was also higher in treated piglets with normal birth-weight. However, these findings did not improve productive traits at birth or following periods in litters from supplemented gilts (parity 1) or sows (parities 2–3). Thus, further research is needed to properly understand the effects of prenatal glutamine supplementation, particularly in traditional swine breeds.60pt Keywords: amino acids; fatty acids; mTOR; parity; pig; pregnancy Author: Marta Vázquez-Gómez, Consolación García-Contreras, Susana Astiz, Laura Torres-Rovira, José Luis Pesantez-Pacheco, Ana Heras-Molina, Teresa Castro Madrigal, Clemente López-Bote, Cristina Óvilo, Antonio González-Bulnes and Beatriz Isabel Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 23 03:48:16 2021 CET ModDate: Tue Mar 23 04:02:15 2021 CET Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 386749 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FOWADO+VnURWPalladioL Type 1 Custom yes yes no 10 0 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edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Gene Expression by Quantitative PCR Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Fat Content and Fatty Acid Composition of Diets and Tissue Samples Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Statistical Analysis Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Children: Item: Title: Gilts and Sows Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Offspring at Birth Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Children: Item: Title: Body Measures and Composition Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Plasma Parameters Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: Gene Expression Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Item: Title: Fatty Acid Composition of Brain, Liver, and Muscle Tissues Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@ed9d034 Item: Title: Postnatal Development Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@6121c9d6 Children: Item: Title: Carcass Traits and Gene Expression at the Slaughterhouse Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@87f383f Item: Title: Fatty Acid Composition of Muscle, Backfat, and Liver Tissues at the Slaughterhouse Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4eb7f003 Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@eafc191 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@612fc6eb Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1060b431 Info: Title: Effects of L-Glutamine Supplementation during the Gestation of Gilts and Sows on the Offspring Development in a Traditional Swine Breed Author: Marta Vázquez-Gómez, Consolación García-Contreras, Susana Astiz, Laura Torres-Rovira, José Luis Pesantez-Pacheco, Ana Heras-Molina, Teresa Castro Madrigal, Clemente López-Bote, Cristina Óvilo, Antonio González-Bulnes and Beatriz Isabel Subject: The use of amino acids during pregnancy, such as glutamine (Gln), seems to be a promising strategy in selected swine breeds to improve the offspring prenatal development. The main goal of the current study was to assess the development of the offspring from parity 1 3 sows of a traditional breed, which were supplemented with 1% glutamine after Day 35 of gestation, under farm conditions. A total of 486 (288 treated) piglets from 78 (46 treated) Iberian sows were used. At birth and slaughterhouse, fatty acid composition, metabolism, and mTOR pathway gene expression were analyzed. At birth, treated newborns showed greater amounts of specific amino acids in plasma, such as glutamine, asparagine, or alanine, and n-3 fatty acids in cellular membranes than control newborns. The expression of genes belonging to mTOR Complex 1 was also higher in treated piglets with normal birth-weight. However, these findings did not improve productive traits at birth or following periods in litters from supplemented gilts (parity 1) or sows (parities 2 3). Thus, further research is needed to properly understand the effects of prenatal glutamine supplementation, particularly in traditional swine breeds.60pt Keywords: amino acids; fatty acids; mTOR; parity; pig; pregnancy Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Mar 23 03:48:16 CET 2021 ModDate: Tue Mar 23 04:02:15 CET 2021 ID: 0x863dace41d1add41975b416ec8f5927f, 0x863dace41d1add41975b416ec8f5927f Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: SKMTQT+VnURWPalladioL-Bold FontSubset: true FirstChar: 161 LastChar: 226 FontDescriptor: FontName: SKMTQT+VnURWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: TVDJPW+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 122 FontDescriptor: FontName: TVDJPW+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: XEYJZU+CMSY10 FontSubset: 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