47cdf2f2c61e408a142f5a3b030c556f pmc_33810110.pdf 2c28f19836d1b37a571af4eed815c8e9f6730329 pmc_33810110.pdf 277a7360896c60eb261c0d2998ec08ee26a2f865c4a5c1bc0c3fe4353b8fb2aa pmc_33810110.pdf Title: Three-Year Clinical Follow-Up of Children Intrauterine Exposed to Zika Virus Subject: Congenital Zika virus (ZIKV) infection may present with a broad spectrum of clinical manifestations. Some sequelae, particularly neurodevelopmental problems, may have a later onset. We conducted a prospective cohort study of 799 high-risk pregnant women who were followed up until delivery. Eighty-three women and/or newborns were considered ZIKV exposed and/or infected. Laboratory diagnosis was made by polymerase chain reaction in the pregnant mothers and their respective newborns, as well as Dengue virus, Chikungunya virus, and ZIKV serology. Serology for toxoplasmosis, rubella, cytomegalovirus, herpes simplex virus, and syphilis infections were also performed in microcephalic newborns. The newborns included in the study were followed up until their third birthday. Developmental delay was observed in nine patients (13.2%): mild cognitive delay in three patients, speech delay in three patients, autism spectrum disorder in two patients, and severe neurological abnormalities in one microcephalic patient; sensorineural hearing loss, three patients and dysphagia, six patients. Microcephaly due to ZIKV occurred in three patients (3.6%). Clinical manifestations can appear after the first year of life in children infected/exposed to ZIKV, emphasizing the need for long-term follow-up. Keywords: Zika virus; congenital infection; microcephaly; cohort studies Author: Rosa Estela Gazeta, Ana Paula Antunes Pascalicchio Bertozzi, Rita de Cássia Aguirre Dezena, Andrea Cristina Botelho Silva, Thamirys Cosmo Gillo Fajardo, Daniel T. Catalan, Maria de Fátima Valente Rizzo, Antonio Fernandes Moron, Antoni Soriano-Arandes, Nuria Sanchez Clemente, Tania Quintella, Dora Fix Ventura, Francisco Max Damico, Valtenice de Cassia Rodrigues de Matos França, Juliana Paula Gomes de Almeida, Ana Laura de Sene Amâncio Zara, Lucas Castro Pires, Cohort Zika vírus Jundiaí and Saulo Duarte Passos Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Mar 24 15:19:42 2021 CET ModDate: Wed Mar 24 15:30:38 2021 CET Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 859751 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- HQHPJI+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 FNNHVC+VnURWPalladioL Type 1 Custom yes yes no 15 0 NJDNTZ+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 QZJBJN+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 MEDHXJ+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 HOWKDX+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 DECPOO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 91 0 DECPPA+PalatinoLinotype TrueType WinAnsi yes yes no 94 0 DEDAAC+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 97 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 105 0 KOSUXD+CMSY10 Type 1 Builtin yes yes no 109 0 CKBPVA+EURM10 Type 1 Builtin yes yes no 113 0 RZRXZK+VnURWPalladioL-Italic Type 1 Custom yes yes no 142 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:24:03 CEST RepresentationInformation: pmc_33810110.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:35 CEST Size: 859751 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 312 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Laboratory Diagnosis Destination: subsection.2.1 Item: Title: Statistical Analysis Destination: subsection.2.2 Item: Title: Ethical Procedures Destination: subsection.2.3 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Three-Year Clinical Follow-Up of Children Intrauterine Exposed to Zika Virus Author: Rosa Estela Gazeta, Ana Paula Antunes Pascalicchio Bertozzi, Rita de Cássia Aguirre Dezena, Andrea Cristina Botelho Silva, Thamirys Cosmo Gillo Fajardo, Daniel T. Catalan, Maria de Fátima Valente Rizzo, Antonio Fernandes Moron, Antoni Soriano-Arandes, Nuria Sanchez Clemente, Tania Quintella, Dora Fix Ventura, Francisco Max Damico, Valtenice de Cassia Rodrigues de Matos França, Juliana Paula Gomes de Almeida, Ana Laura de Sene Amâncio Zara, Lucas Castro Pires, Cohort Zika vírus Jundiaí and Saulo Duarte Passos Subject: Congenital Zika virus (ZIKV) infection may present with a broad spectrum of clinical manifestations. Some sequelae, particularly neurodevelopmental problems, may have a later onset. We conducted a prospective cohort study of 799 high-risk pregnant women who were followed up until delivery. Eighty-three women and/or newborns were considered ZIKV exposed and/or infected. Laboratory diagnosis was made by polymerase chain reaction in the pregnant mothers and their respective newborns, as well as Dengue virus, Chikungunya virus, and ZIKV serology. Serology for toxoplasmosis, rubella, cytomegalovirus, herpes simplex virus, and syphilis infections were also performed in microcephalic newborns. The newborns included in the study were followed up until their third birthday. Developmental delay was observed in nine patients (13.2%): mild cognitive delay in three patients, speech delay in three patients, autism spectrum disorder in two patients, and severe neurological abnormalities in one microcephalic patient; sensorineural hearing loss, three patients and dysphagia, six patients. Microcephaly due to ZIKV occurred in three patients (3.6%). Clinical manifestations can appear after the first year of life in children infected/exposed to ZIKV, emphasizing the need for long-term follow-up. 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