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Title:           The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucu
Subject:         Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with si
Keywords:        RNA silencing suppression, watermelon mosaic potyvirus, cucurbit yellow stunting disease crinivirus, plant virus mixed infection, virus pathogenesis in plants
Author:          Juan José López-Moya
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     Item: 
      Title: The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucurbit yellow stunting disorder virus 
      Children: 
       Item: 
        Title: Introduction
       Item: 
        Title: Materials and Methods
        Children: 
         Item: 
          Title: Plasmid Constructs
         Item: 
          Title: Agroinfiltration and Green Fluorescent Protein Imaging
         Item: 
          Title: Quantitative RT-PCR
         Item: 
          Title: Protein Extract Preparations and Western Blotting
         Item: 
          Title: Subcellular Localization and Co-localization
         Item: 
          Title: BiFC Assays
         Item: 
          Title: Co-immunoprecipitation of Tagged Proteins
       Item: 
        Title: Results
        Children: 
         Item: 
          Title: Confirmation of RNA Silencing Suppression Activity for Transiently Expressed Viral Gene Products
         Item: 
          Title: Combination of Heterologous Gene Products: Negative Effect of WMV P1 on the RSS Activity of CYSDV P25
         Item: 
          Title: Dynamics of Protein Expression in Patches Agroinfiltrated With CYSDV P25 and WMV P1, Both Individually and in Combination
         Item: 
          Title: Dose-Dependent Effect of the Presence of WMV on the RSS Activity of CYSDV P25
         Item: 
          Title: Subcellular Localization of WMV P1 and CYSDV P25 in Nucleus and Cytoplasm
         Item: 
          Title: Interaction of WMV P1 and CYSDV P25
       Item: 
        Title: Discussion
       Item: 
        Title: Data Availability Statement 
       Item: 
        Title: Author Contributions 
     Item: 
      Title: References
   Info: 
    Title: The P1 Protein of Watermelon mosaic virus Compromises the Activity as RNA Silencing Suppressor of the P25 Protein of Cucu
    Author: Juan José López-Moya
    Subject: Mixed viral infections in plants involving a potyvirus and other unrelated virus often result in synergistic effects, with si
    Keywords: RNA silencing suppression, watermelon mosaic potyvirus, cucurbit yellow stunting disease crinivirus, plant virus mixed infection, virus pathogenesis in plants
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       Rect: 350, 366, 434, 356
      Annotation: 
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       Rect: 524, 338, 549, 328
      Annotation: 
       Subtype: Link
       Rect: 314, 328, 378, 318
      Annotation: 
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       Rect: 520, 290, 549, 280
      Annotation: 
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       Rect: 314, 281, 376, 271
      Annotation: 
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       Rect: 379, 252, 437, 242
      Annotation: 
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       Rect: 360, 224, 423, 214
      Annotation: 
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       Rect: 448, 186, 549, 176
      Annotation: 
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       Rect: 492, 119, 549, 109
      Annotation: 
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       Rect: 304, 109, 388, 100
     Thumb: true
  Checksum: 90f7f883
   Type: CRC32
  Checksum: 9f5dda0c20c9e95531ea229aa894ffa4
   Type: MD5
  Checksum: d1763dc721abca254785435ff457c4d2bdde92c8
   Type: SHA-1
  Checksum: 640bd32fc7a644163324082e13954f007beb32fdcd351164e809ae6cb30e048e
   Type: SHA-256