c143962a554c540e0819546488e4ba3b pmc_33919749.pdf 463b00ace5054e1f9f1d5b374c6e6b5f0a5114f4 pmc_33919749.pdf e2c6dc5f9b6eaa7b1f145c367680c54d66178da67f259f16b05dc41d1d4815ac pmc_33919749.pdf Title: Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm Subject: Abdominal aortic aneurysm (AAA) is increasing due to aging of the population and is a major cause of death among the elderly. Ultrasound screening programs are useful in early diagnosis, but aneurysm size is not always a good predictor of rupture. Our aim was to analyze the value of circulating molecules related to oxidative stress and inflammation as new biomarkers to assist the management of AAA. The markers were quantified by ELISA, and their expression in the aneurysmal wall was studied by real-time PCR and by immunostaining. Correlation analysis of the studied markers with aneurysm diameter and peak wall stress (PWS), obtained by finite element analysis, and multivariate regression analysis to assess potential confounding factors were performed. Our study shows an extensive inflammatory infiltration in the aneurysmal wall, mainly composed by T-cells, macrophages and B-cells and altered levels of reactive oxygen species (ROS), IgM, IgG, CD38, GDF15, S100A4 and CD36 in plasma and in the aneurysmal tissue of AAA patients compared with controls. Circulating levels of IgG, CD38 and GDF15 positively correlated with abdominal aortic diameter, and CD38 was correlated with PWS. Our data show that altered levels of IgG, CD38 and GDF15 have potential diagnostic value in the assessment of AAA. Keywords: abdominal aorta aneurysm; biomarkers; inflammation; oxidative stress; prognosis Author: David Sánchez-Infantes, Meritxell Nus, Miquel Navas-Madroñal, Joan Fité, Belén Pérez, Antonio J. Barros-Membrilla, Begoña Soto, José Martínez-González, Mercedes Camacho, Cristina Rodriguez, Ziad Mallat and María Galán Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Apr 14 10:21:17 2021 CEST ModDate: Wed Apr 14 10:37:43 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 9958845 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DAZUAQ+URWPalladioL-Ital Type 1 Custom yes yes no 10 0 CDKCOE+URWPalladioL-Bold Type 1 Custom yes yes no 15 0 SQSBOR+URWPalladioL-Roma Type 1 Custom yes yes no 19 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 23 0 HSNIKI+VnURWPalladioL Type 1 Custom yes yes no 28 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 IFEDSM+CMSY10 Type 1 Builtin yes yes no 78 0 MPUDFL+PazoMath Type 1 Builtin yes yes no 85 0 QPZFHW+EURM10 Type 1 Builtin yes yes no 89 0 ICBUDW+URWPalladioL-BoldItal Type 1 Custom yes yes no 99 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 113 0 ABJHIO+PalatinoLinotype CID TrueType Identity-H yes yes yes 116 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 122 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 125 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 142 0 ABJHIO+PalatinoLinotype CID TrueType Identity-H yes yes yes 145 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 151 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 154 0 ABKGCN+Arial,Italic TrueType WinAnsi yes yes no 171 0 ABKGCO+Arial CID TrueType Identity-H yes yes yes 174 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 180 0 ABJHIO+PalatinoLinotype CID TrueType Identity-H yes yes yes 183 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 189 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 192 0 ABKFOM+Arial,Bold CID TrueType Identity-H yes yes yes 195 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 264 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 267 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 270 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 289 0 ABJHIO+PalatinoLinotype CID TrueType Identity-H yes yes yes 292 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 298 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 301 0 ABJHGO+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 319 0 ABJHIP+PalatinoLinotype TrueType WinAnsi yes yes no 322 0 ABJHKB+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 325 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:20:37 CEST RepresentationInformation: pmc_33919749.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:48 CEST Size: 9958845 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 534 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Human Samples Destination: subsection.2.1 Item: Title: ELISA Kits Destination: subsection.2.2 Item: Title: Total mRNA and Protein Isolation from Tissue Destination: subsection.2.3 Item: Title: Quantitative Real-Time PCR Destination: subsection.2.4 Item: Title: Immunostaining and Histology Destination: subsection.2.5 Item: Title: Reactive Oxygen Species (ROS) Quantification in Plasma Destination: subsection.2.6 Item: Title: Western Blot Destination: subsection.2.7 Item: Title: Finite Element Analysis Destination: subsection.2.8 Item: Title: Statistical Analysis Destination: subsection.2.9 Item: Title: Results Destination: section.3 Children: Item: Title: Immune Infiltrate and Immunoglobulins in Human AAA Destination: subsection.3.1 Item: Title: Vascular and Circulating Levels of Oxidative Stress and Inflammation-Related Biomarkers in AAA Patients Destination: subsection.3.2 Item: Title: Correlation of Biomarker Levels and Preoperative Aortic Diameter Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Oxidative Stress and Inflammatory Markers in Abdominal Aortic Aneurysm Author: David Sánchez-Infantes, Meritxell Nus, Miquel Navas-Madroñal, Joan Fité, Belén Pérez, Antonio J. Barros-Membrilla, Begoña Soto, José Martínez-González, Mercedes Camacho, Cristina Rodriguez, Ziad Mallat and María Galán Subject: Abdominal aortic aneurysm (AAA) is increasing due to aging of the population and is a major cause of death among the elderly. Ultrasound screening programs are useful in early diagnosis, but aneurysm size is not always a good predictor of rupture. Our aim was to analyze the value of circulating molecules related to oxidative stress and inflammation as new biomarkers to assist the management of AAA. The markers were quantified by ELISA, and their expression in the aneurysmal wall was studied by real-time PCR and by immunostaining. Correlation analysis of the studied markers with aneurysm diameter and peak wall stress (PWS), obtained by finite element analysis, and multivariate regression analysis to assess potential confounding factors were performed. Our study shows an extensive inflammatory infiltration in the aneurysmal wall, mainly composed by T-cells, macrophages and B-cells and altered levels of reactive oxygen species (ROS), IgM, IgG, CD38, GDF15, S100A4 and CD36 in plasma and in the aneurysmal tissue of AAA patients compared with controls. Circulating levels of IgG, CD38 and GDF15 positively correlated with abdominal aortic diameter, and CD38 was correlated with PWS. Our data show that altered levels of IgG, CD38 and GDF15 have potential diagnostic value in the assessment of AAA. 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