f1e96fd5cf453c38ead4287ec5f59f37 pmc_33921803.pdf c28ea3768df1f2610b398a0dd2aacb475fc55a49 pmc_33921803.pdf 5d88847e06aa136175df36d54dccf7e8e5f40899148ef968231ce46833acbb85 pmc_33921803.pdf Title: Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established? Subject: Background: The aim of the present study is to describe the occurrence of Blastocystis sp. detection among asymptomatic subjects and patients with irritable bowel syndrome in order to evaluate the potential association between irritable bowel syndrome and the parasitic infection. Methods: Cross-sectional study where adult patients with irritable bowel syndrome diagnosed according to Rome IV criteria were included. A control group was formed by asymptomatic subjects older than 18 years. Exclusion criteria were: immunosuppressive condition or having received any drug with demonstrated activity against Blastocystis sp. within the last 6 months before study inclusion. Epidemiological and clinical information was collected from all included participants. Two stool samples were obtained from all participants: one sample for microscopic examination and one sample for Blastocystis sp. PCR detection. Blastocystis sp. infection was defined by the positivity of any of the diagnostic techniques. Results: Seventy-two participants were included (36 asymptomatic subjects and 36 patients with irritable bowel syndrome). Thirty-five (48.6%) were men, and median age of participants was 34 (IQR 29–49) years. The overall rate of Blastocystis sp. carriage was 27.8% (20/72). The prevalence assessed through microscopic examination was 22.2% (16/72), while the prevalence measured by PCR was 15.3% (11/72). When comparing the presence of Blastocystis sp. between asymptomatic subjects and IBS patients, we did not find any statistically significant difference (36.1% vs. 19.4% respectively, p = 0.114). Conclusions: regarding the occurrence of Blastocystis sp., no differences were found between asymptomatic participants and patients with irritable bowel disease irrespective of the diagnostic technique performed. Keywords: Blastocystis sp.; irritable bowel syndrome; pathogenesis Author: Fernando Salvador, Beatriz Lobo, Lidia Goterris, Carmen Alonso-Cotoner, Javier Santos, Elena Sulleiro, Begoña Bailo, David Carmena, Adrián Sánchez-Montalvá, Pau Bosch-Nicolau, Juan Espinosa-Pereiro, Isabel Fuentes and Israel Molina Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue Apr 20 16:53:03 2021 CEST ModDate: Tue Apr 20 16:57:00 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1740039 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- RNMVOE+VnURWPalladioL Type 1 Custom yes yes no 10 0 DIADTY+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 QZEYAG+URWPalladioL-BoldItal Type 1 Custom yes yes no 20 0 EUDUJH+URWPalladioL-Bold Type 1 Custom yes yes no 24 0 CVAMYA+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 AIQCCL+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 GLCFZC+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 DKZMYW+CMSY10 Type 1 Builtin yes yes no 82 0 RFECCF+EURM10 Type 1 Builtin yes yes no 89 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:20:33 CEST RepresentationInformation: pmc_33921803.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:49 CEST Size: 1740039 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 233 FreeObjects: 3 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Methods Destination: section.2 Children: Item: Title: Statistical and Ethical Issues Destination: subsection.2.1 Item: Title: Microbiological Techniques Destination: subsection.2.2 Item: Title: Results Destination: section.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: Destination: section.A. Item: Title: References Destination: section.A. Info: Title: Blastocystis sp. Carriage and Irritable Bowel Syndrome: Is the Association Already Established? Author: Fernando Salvador, Beatriz Lobo, Lidia Goterris, Carmen Alonso-Cotoner, Javier Santos, Elena Sulleiro, Begoña Bailo, David Carmena, Adrián Sánchez-Montalvá, Pau Bosch-Nicolau, Juan Espinosa-Pereiro, Isabel Fuentes and Israel Molina Subject: Background: The aim of the present study is to describe the occurrence of Blastocystis sp. detection among asymptomatic subjects and patients with irritable bowel syndrome in order to evaluate the potential association between irritable bowel syndrome and the parasitic infection. Methods: Cross-sectional study where adult patients with irritable bowel syndrome diagnosed according to Rome IV criteria were included. A control group was formed by asymptomatic subjects older than 18 years. Exclusion criteria were: immunosuppressive condition or having received any drug with demonstrated activity against Blastocystis sp. within the last 6 months before study inclusion. Epidemiological and clinical information was collected from all included participants. Two stool samples were obtained from all participants: one sample for microscopic examination and one sample for Blastocystis sp. PCR detection. Blastocystis sp. infection was defined by the positivity of any of the diagnostic techniques. Results: Seventy-two participants were included (36 asymptomatic subjects and 36 patients with irritable bowel syndrome). Thirty-five (48.6%) were men, and median age of participants was 34 (IQR 29 49) years. The overall rate of Blastocystis sp. carriage was 27.8% (20/72). The prevalence assessed through microscopic examination was 22.2% (16/72), while the prevalence measured by PCR was 15.3% (11/72). When comparing the presence of Blastocystis sp. between asymptomatic subjects and IBS patients, we did not find any statistically significant difference (36.1% vs. 19.4% respectively, p = 0.114). Conclusions: regarding the occurrence of Blastocystis sp., no differences were found between asymptomatic participants and patients with irritable bowel disease irrespective of the diagnostic technique performed. 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