d6f849a235d265a7a0db1c61017c9295 pmc_33922301.pdf ca591e653f9c68f4b6eadf082d99541703b5b1bd pmc_33922301.pdf 197a7950405f0449131cfcae2a21ba7d9ca3890b12e4ab62094b1e0e26984e7c pmc_33922301.pdf Title: Role of VEGF Polymorphisms in the Susceptibility and Severity of Interstitial Lung Disease Subject: The search for biomarkers that can help to establish an early diagnosis and prognosis of interstitial lung disease (ILD) is of potential interest. VEGF polymorphisms have been implicated in the development of several lung disorders. Consequently, we assessed, for the first time, the role of VEGF polymorphisms in the susceptibility and severity of ILD. A total of 436 Caucasian ILD patients (244 with idiopathic interstitial pneumonias (IIPs) and 192 with non-IIP) and 536 ethnically-matched healthy controls were genotyped for VEGF rs833061, rs1570360, rs2010963, rs3025020, and rs3025039 polymorphisms by TaqMan assays. Pulmonary function tests were collected from all the patients. VEGF serum levels were determined by ELISA in a subgroup of patients. No VEGF genotype, allele, carrier, or haplotype differences were found between ILD patients and controls as well as between IIP and non-IIP patients. However, an association of rs1570360 with IIP in women and also with lung function in IIP patients was found. None of the VEGF polymorphisms were associated with VEGF levels. In conclusion, our results suggest that VEGF does not seem to play a relevant role in ILD, although rs1570360 may influence the severity of ILD in women and a worse outcome in IIP patients. Keywords: interstitial lung disease; idiopathic interstitial pneumonia; vascular endothelial growth factor; biomarker; genetics Author: Sara Remuzgo-Martínez, Fernanda Genre, Verónica Pulito-Cueto, Belén Atienza-Mateo, Víctor Manuel Mora Cuesta, David Iturbe Fernández, Sonia María Fernández Rozas, Leticia Lera-Gómez, Pilar Alonso Lecue, María Piedad Ussetti, Rosalía Laporta, Cristina Berastegui, Amparo Solé, Virginia Pérez, Alicia De Pablo Gafas, Oreste Gualillo, José Manuel Cifrián, Raquel López-Mejías and Miguel Ángel González-Gay Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Mon Apr 26 03:29:02 2021 CEST ModDate: Mon Apr 26 03:31:35 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 604889 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- EIFDAO+VnURWPalladioL Type 1 Custom yes yes no 10 0 GDZUCJ+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 UTZAWN+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 ICXQXE+URWPalladioL-BoldItal Type 1 Custom yes yes no 24 0 POYYXG+URWPalladioL-Roma Type 1 Custom yes yes no 28 0 FFAYKL+VnURWPalladioL-Bold Type 1 Custom yes yes no 32 0 EBFBTV+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 40 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 62 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 66 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 70 0 ZPCLNL+CMSY10 Type 1 Builtin yes yes no 85 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 106 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 110 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 114 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 118 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:20:26 CEST RepresentationInformation: pmc_33922301.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:50 CEST Size: 604889 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 273 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Patients and Controls Destination: subsection.2.1 Item: Title: VEGF Polymorphisms Selection and Genotyping Destination: subsection.2.2 Item: Title: Serum VEGF Determination Destination: subsection.2.3 Item: Title: Statistical Analysis Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Analysis of VEGF Genotype, Allele and Carrier Frequencies Destination: subsection.3.1 Item: Title: Haplotype Analysis of VEGF Destination: subsection.3.2 Item: Title: Association of VEGF Polymorphisms with Pulmonary Function Tests Destination: subsection.3.3 Item: Title: Influence of VEGF Polymorphisms on VEGF Serum Levels Destination: subsection.3.4 Item: Title: Discussion Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Role of VEGF Polymorphisms in the Susceptibility and Severity of Interstitial Lung Disease Author: Sara Remuzgo-Martínez, Fernanda Genre, Verónica Pulito-Cueto, Belén Atienza-Mateo, Víctor Manuel Mora Cuesta, David Iturbe Fernández, Sonia María Fernández Rozas, Leticia Lera-Gómez, Pilar Alonso Lecue, María Piedad Ussetti, Rosalía Laporta, Cristina Berastegui, Amparo Solé, Virginia Pérez, Alicia De Pablo Gafas, Oreste Gualillo, José Manuel Cifrián, Raquel López-Mejías and Miguel Ángel González-Gay Subject: The search for biomarkers that can help to establish an early diagnosis and prognosis of interstitial lung disease (ILD) is of potential interest. VEGF polymorphisms have been implicated in the development of several lung disorders. Consequently, we assessed, for the first time, the role of VEGF polymorphisms in the susceptibility and severity of ILD. A total of 436 Caucasian ILD patients (244 with idiopathic interstitial pneumonias (IIPs) and 192 with non-IIP) and 536 ethnically-matched healthy controls were genotyped for VEGF rs833061, rs1570360, rs2010963, rs3025020, and rs3025039 polymorphisms by TaqMan assays. Pulmonary function tests were collected from all the patients. VEGF serum levels were determined by ELISA in a subgroup of patients. No VEGF genotype, allele, carrier, or haplotype differences were found between ILD patients and controls as well as between IIP and non-IIP patients. However, an association of rs1570360 with IIP in women and also with lung function in IIP patients was found. None of the VEGF polymorphisms were associated with VEGF levels. In conclusion, our results suggest that VEGF does not seem to play a relevant role in ILD, although rs1570360 may influence the severity of ILD in women and a worse outcome in IIP patients. 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