6b49ffca5f74921497bb5fe9ae13fce9 pmc_33923393.pdf 983868c8bbeb92b50ebdec0433d31a82c1c5be20 pmc_33923393.pdf ce6178c0f1b26b9455305efa9865ca89802b880d2ff29ea245d5c3f21c5f668c pmc_33923393.pdf Title: Changes in the Composition and Function of Lipoproteins after Bariatric Surgery in Patients with Severe Obesity Subject: The effect of bariatric surgery on lipid profile and the qualitative characteristics of lipoproteins was analyzed in morbidly obese subjects. Thirteen obese patients underwent bariatric surgery. Plasma samples were obtained before surgery and at 6 and 12 months after the intervention. Thirteen healthy subjects comprised the control group. Lipid profile, hsCRP, and the composition and functional characteristics of VLDL, LDL, and HDL were assessed. At baseline, plasma from subjects with obesity had more triglycerides, VLDLc, and hsCRP, and less HDLc than the control group. These levels progressively normalized after surgery, although triglyceride and hsCRP levels remained higher than those in the controls. The main differences in lipoprotein composition between the obese subjects and the controls were increased apoE in VLDL, and decreased cholesterol and apoJ and increased apoC-III content in HDL. The pro-/anti-atherogenic properties of LDL and HDL were altered in the subjects with obesity at baseline compared with the controls, presenting smaller LDL particles that are more susceptible to modification and smaller HDL particles with decreased antioxidant capacity. Bariatric surgery normalized the composition of lipoproteins and improved the qualitative characteristics of LDL and HDL. In summary, patients with obesity present multiple alterations in the qualitative properties of lipoproteins compared with healthy subjects. Bariatric surgery reverted most of these alterations. Keywords: obesity; bariatric surgery; lipoproteins; lipoprotein function; LDL; HDL; apolipoproteins Author: Idoia Genua, Núria Puig, Inka Miñambres, Sonia Benítez, Pedro Gil, Margarida Grau-Agramunt, Andrea Rivas-Urbina, Carme Balagué, Sonia Fernández-Alanin, Álvaro García-Osuna, Antonio Pérez and José Luis Sánchez-Quesada Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri Apr 16 07:56:52 2021 CEST ModDate: Fri Apr 16 07:59:14 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1596058 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- FJDQIS+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 PLMECB+VnURWPalladioL Type 1 Custom yes yes no 15 0 HWSHJX+URWPalladioL-Ital Type 1 Custom yes yes no 19 0 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PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:09:51 CEST Size: 1596058 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 852 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Study Population Destination: subsection.2.1 Item: Title: Plasma Determinations Destination: subsection.2.2 Item: Title: Lipoprotein Isolation and Composition Destination: subsection.2.3 Item: Title: Lipoprotein Functional Assays Destination: subsection.2.4 Item: Title: Statistical Analysis Destination: subsection.2.5 Item: Title: Results Destination: section.3 Children: Item: Title: Lipid Profile and Inflammation Markers Destination: subsection.3.1 Item: Title: Changes in VLDL Composition Destination: subsection.3.2 Item: Title: Changes in LDL Composition and Function Destination: subsection.3.3 Item: Title: Changes in HDL Composition and Function Destination: subsection.3.4 Item: Title: Distribution of Lp-PLA2 Activity in Plasma Destination: subsection.3.5 Item: Title: Distribution of Apolipoprotein J (apoJ) in Plasma Destination: subsection.3.6 Item: Title: Correlation Analysis Destination: subsection.3.7 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Changes in the Composition and Function of Lipoproteins after Bariatric Surgery in Patients with Severe Obesity Author: Idoia Genua, Núria Puig, Inka Miñambres, Sonia Benítez, Pedro Gil, Margarida Grau-Agramunt, Andrea Rivas-Urbina, Carme Balagué, Sonia Fernández-Alanin, Álvaro García-Osuna, Antonio Pérez and José Luis Sánchez-Quesada Subject: The effect of bariatric surgery on lipid profile and the qualitative characteristics of lipoproteins was analyzed in morbidly obese subjects. Thirteen obese patients underwent bariatric surgery. Plasma samples were obtained before surgery and at 6 and 12 months after the intervention. Thirteen healthy subjects comprised the control group. Lipid profile, hsCRP, and the composition and functional characteristics of VLDL, LDL, and HDL were assessed. At baseline, plasma from subjects with obesity had more triglycerides, VLDLc, and hsCRP, and less HDLc than the control group. These levels progressively normalized after surgery, although triglyceride and hsCRP levels remained higher than those in the controls. The main differences in lipoprotein composition between the obese subjects and the controls were increased apoE in VLDL, and decreased cholesterol and apoJ and increased apoC-III content in HDL. The pro-/anti-atherogenic properties of LDL and HDL were altered in the subjects with obesity at baseline compared with the controls, presenting smaller LDL particles that are more susceptible to modification and smaller HDL particles with decreased antioxidant capacity. Bariatric surgery normalized the composition of lipoproteins and improved the qualitative characteristics of LDL and HDL. In summary, patients with obesity present multiple alterations in the qualitative properties of lipoproteins compared with healthy subjects. Bariatric surgery reverted most of these alterations. 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