2dc5bfcff14f130fd0f63d870887e67e pmc_33925008.pdf 522bc8044cce83b8eb54e577dfc4003c7d03f274 pmc_33925008.pdf b20ffa87d5185a3e813f816089c8c9eaa0bb8250241e47c34cc11d49c7173f3a pmc_33925008.pdf Title: Diagnostic Performance of the Fujifilm SILVAMP TB-LAM in Children with Presumptive Tuberculosis Subject: Current diagnostics for tuberculosis (TB) only manage to confirm a small proportion of children with TB and require respiratory samples, which are difficult to obtain. There is a need for non-invasive biomarker-based tests as an alternative to sputum testing. Fujifilm SILVAMP TB lipoarabinomannan (FujiLAM), a lateral-flow test to detect lipoarabinomannan in urine, is a novel non-sputum-based point-of-care diagnostic reported to have increased sensitivity for the diagnosis of TB among human immunodeficiency virus (HIV)-infected adults. We evaluate the performance of FujiLAM in children with presumptive TB. Fifty-nine children attending a paediatric hospital in Haiti with compatible signs and symptoms of TB were examined using Xpert MTB/RIF, smear microscopy and X-rays, and classified according to the certainty of diagnosis into bacteriologically confirmed TB (n = 5), unconfirmed TB (bacteriologically negative, n = 50) and unlikely TB (n = 4). Healthy children (n = 20) were enrolled as controls. FujiLAM sensitivity and specificity were 60% and 95% among children with confirmed TB. FujiLAM’s high specificity and its characteristics as a point-of-care indicate the test has a good potential for the diagnosis of TB in children. Keywords: tuberculosis; diagnosis; lipoarabinomannan; LAM; children; urine; point-of-care Author: Patricia Comella-del-Barrio, Bárbara Molina-Moya, Jacqueline Gautier, Raquel Villar-Hernández, Mariette Jean Coute Doresca, Beatriz Sallés-Mingels, Lydia Canales-Aliaga, Margareth Narcisse, Tomás M. Pérez-Porcuna, Jacob Creswell, Luis E. Cuevas and José Domínguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Apr 29 10:51:36 2021 CEST ModDate: Thu Apr 29 11:00:11 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 8 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 274843 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ARNGBH+VnURWPalladioL Type 1 Custom yes yes no 10 0 ZNKMSN+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 ZFNFKK+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 GRLZZL+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 IOJCNK+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 XPUSBJ+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 BSDWLC+TimesNewRomanPSMT Type 1C WinAnsi yes yes no 39 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 61 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 65 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 69 0 FFDGWE+CMSY10 Type 1 Builtin yes yes no 81 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 91 0 OQIVQT+EURM10 Type 1 Builtin yes yes no 98 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 03:23:53 CEST RepresentationInformation: pmc_33925008.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-05-24 03:38:17 CEST Size: 274843 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 238 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Results Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Info: Title: Diagnostic Performance of the Fujifilm SILVAMP TB-LAM in Children with Presumptive Tuberculosis Author: Patricia Comella-del-Barrio, Bárbara Molina-Moya, Jacqueline Gautier, Raquel Villar-Hernández, Mariette Jean Coute Doresca, Beatriz Sallés-Mingels, Lydia Canales-Aliaga, Margareth Narcisse, Tomás M. Pérez-Porcuna, Jacob Creswell, Luis E. Cuevas and José Domínguez Subject: Current diagnostics for tuberculosis (TB) only manage to confirm a small proportion of children with TB and require respiratory samples, which are difficult to obtain. There is a need for non-invasive biomarker-based tests as an alternative to sputum testing. Fujifilm SILVAMP TB lipoarabinomannan (FujiLAM), a lateral-flow test to detect lipoarabinomannan in urine, is a novel non-sputum-based point-of-care diagnostic reported to have increased sensitivity for the diagnosis of TB among human immunodeficiency virus (HIV)-infected adults. We evaluate the performance of FujiLAM in children with presumptive TB. Fifty-nine children attending a paediatric hospital in Haiti with compatible signs and symptoms of TB were examined using Xpert MTB/RIF, smear microscopy and X-rays, and classified according to the certainty of diagnosis into bacteriologically confirmed TB (n = 5), unconfirmed TB (bacteriologically negative, n = 50) and unlikely TB (n = 4). Healthy children (n = 20) were enrolled as controls. FujiLAM sensitivity and specificity were 60% and 95% among children with confirmed TB. FujiLAM’s high specificity and its characteristics as a point-of-care indicate the test has a good potential for the diagnosis of TB in children. 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