34c14219d67d57df8ed4cf7093b3870f pmc_33957869.pdf dea6b28ccb3715e3e26cfb12dad0cb3bc26397df pmc_33957869.pdf c3a3df888697cee8dc5e2a4d2847413161fe4e6899ba9a0ae741c993c855c85a pmc_33957869.pdf Title: Long-read metagenomics retrieves complete single-contig bacterial genomes from canine feces Subject: BMC Genomics, 2021, doi:10.1186/s12864-021-07607-0 Keywords: Long-read metagenomics,Gastrointestinal microbiome,Fecal microbiome,Long-reads,Nanopore,Canine microbiome,Dog microbiome,Metagenome-assembled genomes,Sutterella Author: Anna Cuscó Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.0.0 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue May 4 21:38:00 2021 CEST ModDate: Thu May 6 14:58:02 2021 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 790.866 pts Page rot: 0 File size: 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Destination: sectionSec3 Item: Title: Raw reads: pre-processing, quality control, and taxonomic analyses Destination: sectionSec4 Item: Title: Metagenomics assembly and polishing Destination: sectionSec5 Item: Title: Characterization of the high-quality MAGs Destination: sectionSec6 Item: Title: Functional and Pangenomics analysis of the HQ MAGs Destination: sectionSec7 Item: Title: Results Destination: sectionSec8 Children: Item: Title: HMW DNA extraction for longer reads and larger contigs Destination: sectionSec9 Item: Title: Metagenomics assembly with different subsets followed by frameshift aware correction retrieved eight high-quality MAGs Destination: sectionSec10 Item: Title: High-quality MAGs of the canine fecal microbiome improved previous genome assemblies Destination: sectionSec11 Item: Title: Screening of previous microbiome studies revealed the first potential genome assembly for Sutterella stercoricanis Destination: sectionSec12 Item: Title: Long reads provide genomic context and enable capturing mobilome functions and antimicrobial-resistant genes Destination: sectionSec13 Item: Title: Discussion Destination: sectionSec14 Item: Title: Abbreviations Destination: sectionabr1 Item: Title: Supplementary Information Destination: sectionSec15 Item: Title: Acknowledgments Destination: section1 Item: Title: Authors’ contributions Destination: section2 Item: Title: Funding Destination: section3 Item: Title: Availability of data and materials Destination: section4 Item: Title: Declarations Destination: section5 Item: Title: Ethics approval and consent to participate Destination: sectionFPar1 Item: Title: Consent for publication Destination: sectionFPar2 Item: Title: Competing interests Destination: sectionFPar3 Item: Title: Author details Destination: section6 Item: Title: References Destination: sectionBib1 Item: Title: Publisher’s Note Destination: ArtNote1 Info: Title: Long-read metagenomics retrieves complete single-contig bacterial genomes from canine feces Author: Anna Cuscó Subject: BMC Genomics, 2021, doi:10.1186/s12864-021-07607-0 Keywords: Long-read metagenomics,Gastrointestinal microbiome,Fecal microbiome,Long-reads,Nanopore,Canine microbiome,Dog microbiome,Metagenome-assembled genomes,Sutterella Creator: Arbortext Advanced Print Publisher 9.1.440/W Unicode Producer: Acrobat Distiller 10.0.0 (Windows); modified using iText® 5.3.5 ©2000-2012 1T3XT BVBA (SPRINGER SBM; licensed version) CreationDate: Tue May 04 21:38:00 CEST 2021 ModDate: Thu May 06 14:58:02 CEST 2021 ID: 0x1cb7f1ad261f05aabf74c451834d960b, 0xa7c3328c782a927e28e6e93b5ca8c508 Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 176 ImageHeight: 182 BitsPerSample: 8 BitsPerSampleUnit: integer Intent: Perceptual Image: NisoImageMetadata: FormatName: image/png ImageWidth: 688 ImageHeight: 126 BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: 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