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Keywords: high pressure processing, Listeria monocytogenes, mathematical modeling, membrane damage, repair process, fluorescence microscopy Author: Nadav Bar Creator: LaTeX with hyperref package + hypdvips Producer: dvips + MiKTeX GPL Ghostscript 9.0 CreationDate: Wed May 12 07:39:11 2021 CEST ModDate: Wed May 12 10:03:18 2021 CEST Custom Metadata: no Metadata Stream: yes Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.26 x 779.52 pts Page rot: 0 File size: 1650734 bytes Optimized: no PDF version: 1.6 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- PRPABV+HelveticaNeueLTStd-Bd Type 1C Custom yes yes no 35 0 IUNGCR+HelveticaNeueLTStd-Lt Type 1C Custom yes yes no 40 0 OXKQIT+HelveticaNeueLTStd-BdIt Type 1C WinAnsi yes yes no 53 0 WTXERX+HelveticaNeueLTStd-LtIt Type 1C WinAnsi yes yes no 55 0 RDWXHZ+HelveticaNeueLTStd-MdIt Type 1C WinAnsi yes yes no 60 0 KCFCLT+MTSY Type 1C Custom yes yes yes 62 0 KPMRVD+MinionPro-Bold Type 1C Custom yes yes no 116 0 ADEDLH+MinionPro-Regular Type 1C Custom yes yes no 70 0 ZHGCWV+HelveticaNeueLTStd-Md Type 1C WinAnsi yes yes no 138 0 BJWTPZ+MinionPro-It Type 1C WinAnsi yes yes no 90 0 CKZXRS+RMTMI Type 1C Custom yes yes yes 157 0 QAMUEV+MTSYB Type 1C Custom yes yes no 321 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:16:26 CEST RepresentationInformation: pmc_34054742.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:13:56 CEST Size: 1650734 Format: PDF Version: 1.6 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 499 FreeObjects: 19 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseOutlines Language: en Outlines: Item: Title: A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data Destination: ariclecounter.1 Children: Item: Title: 1. Introduction Destination: section.0.1 Item: Title: 2. Materials and Methods Destination: section.0.2 Children: Item: Title: 2.1. Experiments Destination: subsection.0.2.1 Children: Item: Title: 2.1.1. Bacterial Strain and Culture Conditions Destination: subsubsection.0.2.1.1 Item: Title: 2.1.2. High Pressure Processing (HPP) Destination: subsubsection.0.2.1.2 Item: Title: 2.1.3. Cell Preparation and Fluorescence Microscopy Analysis Destination: subsubsection.0.2.1.3 Item: Title: 2.1.4. Culture-Based Cell Counting Destination: subsubsection.0.2.1.4 Item: Title: 2.2. Post-processing of Microscopy Data Destination: subsection.0.2.2 Item: Title: 2.3. Statistical Analysis Destination: subsection.0.2.3 Item: Title: 2.4. A Computational Model to Describe Membrane Recovery Destination: subsection.0.2.4 Item: Title: 3. Results Destination: section.0.3 Children: Item: Title: 3.1. Monitoring the Diffusion of PI Molecules Into Damaged Bacterial Cells Through Membrane Pores Destination: subsection.0.3.1 Item: Title: 3.2. Estimation of the Pore Area Destination: subsection.0.3.2 Item: Title: 3.3. Population Growth Behavior of Pressure-Treated L. monocytogenes Destination: subsection.0.3.3 Item: Title: 4. Discussion Destination: section.0.4 Item: Title: 5. Conclusion Destination: section.0.5 Item: Title: Data Availability Statement Destination: section*.18 Item: Title: Author Contributions Destination: section*.19 Item: Title: Funding Destination: section*.20 Item: Title: Acknowledgments Destination: section*.21 Item: Title: Supplementary Material Destination: section*.22 Item: Title: References Destination: section*.23 Info: Title: A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data Author: Nadav Bar Subject: The effects of environmental stresses on microorganisms have been well-studied, and cellular responses to stresses such as heat, cold, acids, and salts have been extensively discussed. Keywords: high pressure processing, Listeria monocytogenes, mathematical modeling, membrane damage, repair process, fluorescence microscopy Creator: LaTeX with hyperref package + hypdvips Producer: dvips + MiKTeX GPL Ghostscript 9.0 CreationDate: Wed May 12 08:09:11 CEST 2021 ModDate: Wed May 12 10:33:18 CEST 2021 ID: 0x23864f380080f197dc4aa496f59618a7, 0xe2f3ee120d050246a8180881136ce60f Filters: FilterPipeline: FlateDecode FilterPipeline: DCTDecode Images: Image: NisoImageMetadata: FormatName: image/png ImageWidth: 279 ImageHeight: 279 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Filter: FlateDecode Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 945 ImageHeight: 1008 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: NisoImageMetadata: FormatName: image/jpg CompressionScheme: JPEG ImageWidth: 945 ImageHeight: 1740 ColorSpace: RGB BitsPerSample: 8 BitsPerSampleUnit: integer Interpolate: true Image: 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Nadav Bar A Diffusion Model to Quantify Membrane Repair Process in Listeria monocytogenes Exposed to High Pressure Processing Based on Fluorescence Microscopy Data high pressure processing Listeria monocytogenes mathematical modeling membrane damage repair process fluorescence microscopy high pressure processing, Listeria monocytogenes, mathematical modeling, membrane damage, repair process, fluorescence microscopy dvips + MiKTeX GPL Ghostscript 9.0 uuid:226f4552-dbbf-4740-96b7-a0df02e8baaf uuid:0033cd19-3562-4abf-b264-dcae90572dc4 Pages: Page: Label: 1 Annotations: Annotation: Subtype: Link Rect: 43, 719, 160, 755 Annotation: Subtype: Link Rect: 474, 736, 551, 744 Annotation: Subtype: Link Rect: 476, 728, 519, 736 Annotation: Subtype: Link Rect: 519, 728, 533, 736 Annotation: Subtype: Link Rect: 533, 728, 551, 736 Annotation: Subtype: Link Rect: 449, 720, 551, 728 Annotation: Subtype: Link Rect: 521, 678, 551, 709 Annotation: Subtype: Link Rect: 43, 30, 118, 38 Annotation: Subtype: 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456, 296, 476, 307 Destination: External Annotation: Subtype: Link Rect: 344, 261, 398, 273 Destination: External Annotation: Subtype: Link Rect: 401, 261, 421, 273 Destination: External Annotation: Subtype: Link Rect: 427, 261, 523, 273 Destination: External Annotation: Subtype: Link Rect: 528, 261, 548, 273 Destination: External Annotation: Subtype: Link Rect: 409, 216, 452, 227 Destination: External Annotation: Subtype: Link Rect: 455, 216, 475, 227 Destination: External Annotation: Subtype: Link Rect: 478, 216, 523, 227 Destination: External Annotation: Subtype: Link Rect: 525, 216, 546, 227 Destination: External Annotation: Subtype: Link Rect: 425, 181, 526, 193 Destination: External Annotation: Subtype: Link Rect: 529, 181, 549, 193 Destination: External Annotation: Subtype: Link Rect: 304, 170, 370, 181 Destination: External Annotation: Subtype: Link Rect: 373, 170, 394, 181 Destination: External Annotation: Subtype: Link Rect: 43, 29, 118, 37 Annotation: Subtype: Link Rect: 122, 29, 182, 37 Annotation: Subtype: Link Rect: 433, 29, 551, 38 Page: Label: 12 Sequence: 12 Annotations: Annotation: Subtype: Link Rect: 231, 651, 288, 662 Destination: External Annotation: Subtype: Link Rect: 43, 640, 64, 651 Destination: External Annotation: Subtype: Link Rect: 66, 640, 172, 651 Destination: External Annotation: Subtype: Link Rect: 175, 640, 195, 651 Destination: External Annotation: Subtype: Link Rect: 82, 594, 144, 605 Destination: External Annotation: Subtype: Link Rect: 148, 594, 168, 605 Destination: External Annotation: Subtype: Link Rect: 172, 594, 230, 605 Destination: External Annotation: Subtype: Link Rect: 234, 594, 254, 605 Destination: External Annotation: Subtype: Link Rect: 258, 594, 93, 594 Destination: External Annotation: Subtype: Link Rect: 95, 582, 115, 594 Destination: External Annotation: Subtype: Link Rect: 187, 548, 239, 559 Destination: External Annotation: Subtype: Link Rect: 241, 548, 261, 559 Destination: External Annotation: Subtype: Link Rect: 264, 548, 64, 548 Destination: External Annotation: Subtype: Link Rect: 67, 536, 87, 548 Destination: External Annotation: Subtype: Link Rect: 181, 502, 288, 514 Destination: External Annotation: Subtype: Link Rect: 43, 491, 64, 502 Destination: External Annotation: Subtype: Link Rect: 47, 339, 81, 350 Destination: External Annotation: Subtype: Link Rect: 304, 522, 550, 533 Annotation: Subtype: Link Rect: 304, 511, 413, 522 Annotation: Subtype: Link Rect: 552, 84, 303, 108 Annotation: Subtype: Link Rect: 43, 29, 118, 37 Annotation: Subtype: Link Rect: 122, 29, 182, 37 Annotation: Subtype: Link Rect: 433, 29, 551, 38 Page: Label: 13 Sequence: 13 Annotations: Annotation: Subtype: Link Rect: 66, 659, 171, 668 Annotation: Subtype: Link Rect: 66, 623, 174, 630 Annotation: Subtype: Link Rect: 106, 583, 210, 593 Annotation: Subtype: Link Rect: 207, 566, 274, 573 Annotation: Subtype: Link Rect: 66, 537, 166, 545 Annotation: Subtype: Link Rect: 189, 508, 267, 517 Annotation: Subtype: Link Rect: 117, 479, 202, 488 Annotation: Subtype: Link Rect: 186, 452, 285, 460 Annotation: Subtype: Link Rect: 144, 423, 224, 431 Annotation: Subtype: Link Rect: 183, 395, 247, 403 Annotation: Subtype: Link Rect: 177, 366, 286, 375 Annotation: Subtype: Link Rect: 172, 339, 283, 346 Annotation: Subtype: Link Rect: 66, 311, 124, 318 Annotation: Subtype: Link Rect: 138, 281, 241, 289 Annotation: Subtype: Link Rect: 66, 235, 155, 242 Annotation: Subtype: Link Rect: 66, 197, 155, 204 Annotation: Subtype: Link Rect: 292, 147, 52, 168 Annotation: Subtype: Link Rect: 118, 121, 198, 129 Annotation: Subtype: Link Rect: 95, 93, 175, 100 Annotation: Subtype: Link Rect: 363, 689, 470, 696 Annotation: Subtype: Link Rect: 417, 650, 521, 659 Annotation: Subtype: Link Rect: 356, 621, 451, 631 Annotation: Subtype: Link Rect: 327, 594, 396, 602 Annotation: Subtype: Link Rect: 366, 557, 438, 564 Annotation: Subtype: Link Rect: 422, 509, 481, 517 Annotation: Subtype: Link Rect: 327, 470, 431, 479 Annotation: Subtype: Link Rect: 327, 432, 431, 441 Annotation: Subtype: Link Rect: 418, 404, 522, 413 Annotation: Subtype: Link Rect: 363, 339, 470, 346 Annotation: Subtype: Link Rect: 327, 301, 400, 308 Annotation: Subtype: Link Rect: 407, 262, 503, 271 Annotation: Subtype: Link Rect: 327, 224, 415, 233 Annotation: Subtype: Link Rect: 433, 187, 522, 195 Annotation: Subtype: Link Rect: 372, 91, 551, 101 Annotation: Subtype: Link Rect: 314, 82, 416, 91 Annotation: Subtype: Link Rect: 43, 28, 118, 36 Annotation: Subtype: Link Rect: 122, 28, 182, 36 Annotation: Subtype: Link Rect: 433, 28, 551, 37 Page: Label: 14 Sequence: 14 Annotations: Annotation: Subtype: Link Rect: 172, 699, 279, 706 Annotation: Subtype: Link Rect: 95, 680, 211, 687 Annotation: Subtype: Link Rect: 66, 613, 174, 621 Annotation: Subtype: Link Rect: 66, 546, 144, 555 Annotation: Subtype: Link Rect: 183, 517, 248, 526 Annotation: Subtype: Link Rect: 66, 471, 152, 479 Annotation: Subtype: Link Rect: 66, 413, 171, 422 Annotation: Subtype: Link Rect: 130, 385, 226, 394 Annotation: Subtype: Link Rect: 66, 358, 194, 365 Annotation: Subtype: Link Rect: 327, 688, 426, 697 Annotation: Subtype: Link Rect: 552, 650, 313, 669 Annotation: Subtype: Link Rect: 363, 632, 420, 640 Annotation: Subtype: Link Rect: 432, 604, 540, 611 Annotation: Subtype: Link Rect: 353, 574, 412, 583 Annotation: Subtype: Link Rect: 552, 537, 313, 555 Annotation: Subtype: Link Rect: 406, 508, 495, 517 Annotation: Subtype: Link Rect: 552, 470, 313, 489 Annotation: Subtype: Link Rect: 316, 395, 343, 402 Annotation: Subtype: Link Rect: 345, 395, 377, 402 Annotation: Subtype: Link Rect: 379, 395, 414, 403 Annotation: Subtype: Link Rect: 416, 395, 439, 402 Annotation: Subtype: Link Rect: 441, 394, 470, 403 Annotation: Subtype: Link Rect: 43, 29, 118, 37 Annotation: Subtype: Link Rect: 122, 29, 182, 37 Annotation: Subtype: Link Rect: 433, 29, 551, 38 Checksum: b2daa7ec Type: CRC32 Checksum: ae4dde9447c87e3fc619e70d513d7bf5 Type: MD5 Checksum: adbeb2778db7d4067856a0db8b06c7c46e0b8de4 Type: SHA-1 Checksum: 6202a9a30efa3dbb221bb952b5f8506231c66825de3b805763583b2a28b8bff4 Type: SHA-256