976d638921e6ee89d41fcf31ef2ec941 pmc_34064366.pdf 2f0098de3b6e64fade0a2f74131679237934bb57 pmc_34064366.pdf 29102b2fc6c2e7052c4094fc82be698c583b3bc9529189433b6bf472ada3e62e pmc_34064366.pdf Title: Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature Subject: An increased risk of cardiovascular morbidity has been reported in lithiasic patients. In this context, endothelial dysfunction (ED), an earlier status of atherogenesis, has been identified in hyperoxaluria rat models of urolithiasis. Objective: The purpose of this study was to determine the endothelial vascular function in patients with urolithiasis in relation to systemic inflammatory, oxidative stress, and vascular function serum markers. Methods: A cross-sectional study was performed between 27 urolithiasic patients, matched for age and sex, with 27 healthy patients. Endothelial function was assessed by measuring flow-mediated dilation (Celermajer method). Fasting blood was collected to determine metabolic parameters (glucose and lipid profile), along with serum CRP, IL-6, MDA, ADMA, and VCAM-1. Results: Both the control and urolithiasis groups were homogenous in anthropometric, exploration, and general laboratory measures. Flow-mediated dilation (%FMD) was 11.85% (SE: 2.78) lower in the lithiasis group (p < 0.001). No significant differences were achieved between groups when CRP, IL-6, MDA, ADMA, and VCAM-1 were compared, although slightly higher values of CRP, ADMA, and VCAM-1 were detected in the lithiasic group. A correlation was not reached in any of the serum markers when they were related to flow-mediated values, although a slight negative correlation trend was observed in MDA, VCAM-1, and IL-6 values. Conclusions: Endothelial dysfunction constitutes an important disorder related to urolithiasis patients. It must be considered as an early feature responsible for future cardiovascular events. Our study did not find a significant association between inflammatory, oxidative stress, endothelial serum markers, and flow-mediated dilation. Keywords: endothelial dysfunction; oxidative stress; urolithiasis Author: Javier Sáenz-Medina, María Martinez, Silvia Rosado, Manuel Durán, Dolores Prieto and Joaquín Carballido Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue May 4 11:10:04 2021 CEST ModDate: Tue May 4 11:12:22 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 10 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 976299 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- ORWJHQ+VnURWPalladioL Type 1 Custom yes yes no 10 0 HJEJBW+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 BLYYIS+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 DBNRIR+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 SSNIUI+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 KUDXZB+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 36 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 58 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 62 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 66 0 CKBPVA+EURM10 Type 1 Builtin yes yes no 78 0 JDUPKS+CMSY10 Type 1 Builtin yes yes no 85 0 QLBIEB+URWPalladioL-BoldItal Type 1 Custom yes yes no 89 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 101 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 105 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 109 0 BCDJEE+PalatinoLinotype-Roman CID TrueType Identity-H yes yes yes 113 0 BCDKEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 120 0 BCDLEE+CambriaMath TrueType WinAnsi yes yes no 124 0 BCDMEE+Calibri TrueType WinAnsi yes yes no 130 0 BCDEEE+PalatinoLinotype-Bold TrueType WinAnsi yes yes no 146 0 BCDFEE+PalatinoLinotype-Roman TrueType WinAnsi yes yes no 150 0 BCDGEE+PalatinoLinotype-Italic TrueType WinAnsi yes yes no 154 0 BCDKEE+PalatinoLinotype-BoldItalic TrueType WinAnsi yes yes no 158 0 BCDLEE+CambriaMath TrueType WinAnsi yes yes no 162 0 BCDNEE+Arial-BoldMT TrueType WinAnsi yes yes no 167 0 ArialMT TrueType WinAnsi no no no 171 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:16:09 CEST RepresentationInformation: pmc_34064366.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:13:58 CEST Size: 976299 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 313 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Material and Methods Destination: section.2 Children: Item: Title: Study Design Destination: subsection.2.1 Item: Title: Assessment of Endothelial Function Destination: subsection.2.2 Item: Title: Assays Destination: subsection.2.3 Item: Title: Data Presentation and Statistical Analysis Destination: subsection.2.4 Item: Title: Results Destination: section.3 Children: Item: Title: Demographic and Basal Characteristics Destination: subsection.3.1 Item: Title: Endothelial Function Destination: subsection.3.2 Item: Title: Inflammation, Oxidative Stress, and Endothelial Dysfunction Markers Destination: subsection.3.3 Item: Title: Discussion Destination: section.4 Item: Title: Conclusions Destination: section.5 Item: Title: References Destination: section.5 Info: Title: Urolithiasis Develops Endothelial Dysfunction as a Clinical Feature Author: Javier Sáenz-Medina, María Martinez, Silvia Rosado, Manuel Durán, Dolores Prieto and Joaquín Carballido Subject: An increased risk of cardiovascular morbidity has been reported in lithiasic patients. In this context, endothelial dysfunction (ED), an earlier status of atherogenesis, has been identified in hyperoxaluria rat models of urolithiasis. Objective: The purpose of this study was to determine the endothelial vascular function in patients with urolithiasis in relation to systemic inflammatory, oxidative stress, and vascular function serum markers. Methods: A cross-sectional study was performed between 27 urolithiasic patients, matched for age and sex, with 27 healthy patients. Endothelial function was assessed by measuring flow-mediated dilation (Celermajer method). Fasting blood was collected to determine metabolic parameters (glucose and lipid profile), along with serum CRP, IL-6, MDA, ADMA, and VCAM-1. Results: Both the control and urolithiasis groups were homogenous in anthropometric, exploration, and general laboratory measures. Flow-mediated dilation (%FMD) was 11.85% (SE: 2.78) lower in the lithiasis group (p < 0.001). No significant differences were achieved between groups when CRP, IL-6, MDA, ADMA, and VCAM-1 were compared, although slightly higher values of CRP, ADMA, and VCAM-1 were detected in the lithiasic group. A correlation was not reached in any of the serum markers when they were related to flow-mediated values, although a slight negative correlation trend was observed in MDA, VCAM-1, and IL-6 values. Conclusions: Endothelial dysfunction constitutes an important disorder related to urolithiasis patients. It must be considered as an early feature responsible for future cardiovascular events. Our study did not find a significant association between inflammatory, oxidative stress, endothelial serum markers, and flow-mediated dilation. 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