441b2fbe9b3fe840693812ca3a42574f pmc_34065547.pdf 45e09ae5e69efb1528c7d2d898afa48548f231a4 pmc_34065547.pdf 2c0c7a2d61cc7ef504b29aa75b858e839e7f41205a93292df88617e10ad91a32 pmc_34065547.pdf Title: Sow Vaccination with a Protein Fragment against Virulent Glaesserella (Haemophilus) parasuis Modulates Immunity Traits in Their Offspring Subject: Glaesserella (Haemophilus) parasuis, an early colonizer of the nasal cavity in piglets, is a highly heterogeneous species, comprising both commensal and virulent strains. Virulent G. parasuis strains can cause fibrinous polyserositis called Glässer’s disease. Colostrum is a source of passive immunity for young piglets. When vaccinating sows, protective antibodies are transferred to their offspring through the colostrum. Here, sow vaccination was performed with a protein fragment, F4, from the outer membrane trimeric autotransporters VtaAs exclusively found in virulent G. parasuis. Piglets were allowed to suckle for 3 weeks, following which a challenge with two virulent strains of G. parasuis was performed. A group of nonvaccinated sows and their piglets were included as a control. Antibodies against F4 were confirmed using ELISA in the vaccinated sows and their offspring before the G. parasuis challenge. Compared to the control group, F4-vaccination also resulted in an increased level of serum TGF- both in vaccinated sows and in their offspring at early time points of life. After the challenge, a lower body temperature and a higher weight were observed in the group of piglets from vaccinated sows. One piglet from the non-vaccinated group succumbed to the infection, but no other significant differences in clinical signs were noticed. At necropsy, performed 2 weeks after the virulent challenge, the level of surfactant protein D (SP-D) in bronchoalveolar lavage was higher in the piglets from vaccinated sows. Vaccination did not inhibit the nasal colonization of the piglets by the challenge strains. Keywords: swine; bacteria; disease; vaccine; Glaesserella parasuis Author: Sergi López-Serrano, Carlos Neila-Ibáñez, Mar Costa-Hurtado, Yasser Mahmmod, Jorge Martínez-Martínez, Iván José Galindo-Cardiel, Ayub Darji, Fernando Rodríguez, Marina Sibila and Virginia Aragon Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri May 21 10:23:32 2021 CEST ModDate: Fri May 21 10:23:32 2021 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 14 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1364720 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- JLEEZV+URWPalladioL-Ital Type 1 Custom yes yes no 140 0 GJKCQE+URWPalladioL-Bold Type 1 Custom yes yes no 141 0 ZYVVOD+URWPalladioL-BoldItal Type 1 Custom yes yes no 142 0 JYPLJJ+VnURWPalladioL-Bold Type 1 Custom yes yes no 143 0 IXGQNB+URWPalladioL-Roma Type 1 Custom yes yes no 144 0 UKXHGC+VnURWPalladioL Type 1 Custom yes yes no 147 0 VVSYYB+EURM10 Type 1 Builtin yes yes no 148 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 167 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 168 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 169 0 NCKHBC+CMSY10 Type 1 Builtin yes yes no 188 0 CRJHCU+PazoMath-Italic Type 1 Builtin yes yes no 222 0 Jhove (Rel. 1.28.0, 2023-05-18) Date: 2024-10-02 03:16:00 CEST RepresentationInformation: pmc_34065547.pdf ReportingModule: PDF-hul, Rel. 1.12.4 (2023-03-16) LastModified: 2023-03-31 12:13:59 CEST Size: 1364720 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false 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