5ca5ddcf8ba28c02b7fe0cbaa6f355c2 pmc_34066914.pdf 6513f25342f733a66ee107fe828697b859c94371 pmc_34066914.pdf 3bbdddca9fcab9958ea3cc22aa8c566644aeec323e9976f5aba31c77d48378c7 pmc_34066914.pdf Title: Effect of Finishing Diet and Lairage Time on Steers Welfare in Uruguay Subject: The objective of this experiment was to evaluate the effect of two different pasture-based finishing strategies and lairage time on steers welfare in Uruguayan conditions. Sixty Hereford (H) and Braford (B) steers were assigned to two different diets for finishing purposes: (D1) native pasture plus corn grain (1% of live weight) (H n = 15, B n = 15) and (D2) high-quality pasture (H n = 15, B n = 15). The average daily gain was registered every 14 days, and temperaments were individually assessed one week before slaughter by three individual tests: crush score, flight time and exit speed, building a multicriterial temperament index (TIndex). Animals were slaughtered the same day in two groups (50% from D1 and 50% from D2 in each group) after traveling for 3.5 h and staying 15 (long lairage) and 3 h (short lairage) in the lairage pens, respectively. The behaviors were observed during lairage, and physiological indicators were used to assess stress at the farm after transport, after lairage and at slaughter. Bruises incidence and final pH were registered at the abattoir as a means of assessing the overall animal welfare. Calmer animals had higher average daily gains with no differences either between diets or between breeds. Calmer animals also had a lower stress response during all preslaughter stages, regardless of the time in lairage. Transport did not imply psychological stress (cortisol) for any slaughter group, but physical stress was evident after transport in both groups through NEFA and CPK increases. Bruise incidences did not differ between lairage groups. The short lairage group did not have enough time to cope with the environment before slaughter, with the consequent deleterious effects on the carcass pH. Animals from the long lairage group had a higher metabolic response shown through NEFA values, but they had enough time to rest and recover overnight, reaching final pH values lower than 5.8, considered the upper limit of the normal range. According to this experiment, with pasture-based animals without fasting on the farm and after 3.5 h of transportation, a resting period of 15 h in lairage should be better than a 3-h one.+24pt Keywords: stress response; transport in cattle; lairage time; temperament Author: Marcia del Campo Gigena, Juan Manuel Soares de Lima, Gustavo Brito, Xavier Manteca, Pilar Hernández and Fabio Montossi Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri May 7 05:38:50 2021 CEST ModDate: Fri May 7 05:42:18 2021 CEST Custom Metadata: no Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 19 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1023723 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- DUFXDP+VnURWPalladioL Type 1 Custom yes yes no 10 0 UMYSYF+URWPalladioL-Ital Type 1 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valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 522 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: section.1 Item: Title: Materials and Methods Destination: section.2 Children: Item: Title: Field Determinations Destination: subsection.2.1 Children: Item: Title: Productivity Destination: subsubsection.2.1.1 Item: Title: Temperament Destination: subsubsection.2.1.2 Item: Title: Health Status Destination: subsubsection.2.1.3 Item: Title: Transport and Slaughter Plant Destination: subsection.2.2 Children: Item: Title: Physiological Indicators Destination: subsubsection.2.2.1 Item: Title: Behavior in Lairage Pen Destination: subsubsection.2.2.2 Item: Title: Carcass Traits Indicators at the Abattoir as a Means of Assessing Overall Animal Welfare Destination: subsubsection.2.2.3 Item: Title: Statistical Analysis Destination: subsection.2.3 Item: Title: Results and Discussion Destination: section.3 Children: Item: Title: Field Determinations Destination: subsection.3.1 Children: Item: Title: Productivity Destination: subsubsection.3.1.1 Item: Title: Temperament and ADG Destination: subsubsection.3.1.2 Item: Title: Health Status Destination: subsubsection.3.1.3 Item: Title: Transport and Slaughter Plant Destination: subsection.3.2 Children: Item: Title: Physiological Indicators Destination: subsubsection.3.2.1 Item: Title: Behavior in Lairage Pen Destination: subsubsection.3.2.2 Item: Title: Carcass Traits Indicators at the Abattoir as a Means of Assessing Overall Animal Welfare Destination: subsubsection.3.2.3 Item: Title: Conclusions Destination: section.4 Item: Title: References Destination: section.4 Info: Title: Effect of Finishing Diet and Lairage Time on Steers Welfare in Uruguay Author: Marcia del Campo Gigena, Juan Manuel Soares de Lima, Gustavo Brito, Xavier Manteca, Pilar Hernández and Fabio Montossi Subject: The objective of this experiment was to evaluate the effect of two different pasture-based finishing strategies and lairage time on steers welfare in Uruguayan conditions. Sixty Hereford (H) and Braford (B) steers were assigned to two different diets for finishing purposes: (D1) native pasture plus corn grain (1% of live weight) (H n = 15, B n = 15) and (D2) high-quality pasture (H n = 15, B n = 15). The average daily gain was registered every 14 days, and temperaments were individually assessed one week before slaughter by three individual tests: crush score, flight time and exit speed, building a multicriterial temperament index (TIndex). Animals were slaughtered the same day in two groups (50% from D1 and 50% from D2 in each group) after traveling for 3.5 h and staying 15 (long lairage) and 3 h (short lairage) in the lairage pens, respectively. The behaviors were observed during lairage, and physiological indicators were used to assess stress at the farm after transport, after lairage and at slaughter. Bruises incidence and final pH were registered at the abattoir as a means of assessing the overall animal welfare. Calmer animals had higher average daily gains with no differences either between diets or between breeds. Calmer animals also had a lower stress response during all preslaughter stages, regardless of the time in lairage. Transport did not imply psychological stress (cortisol) for any slaughter group, but physical stress was evident after transport in both groups through NEFA and CPK increases. Bruise incidences did not differ between lairage groups. The short lairage group did not have enough time to cope with the environment before slaughter, with the consequent deleterious effects on the carcass pH. Animals from the long lairage group had a higher metabolic response shown through NEFA values, but they had enough time to rest and recover overnight, reaching final pH values lower than 5.8, considered the upper limit of the normal range. 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