d7cd410d32d099b5d2a7129d27fcc10a pmc_34069239.pdf 08153bcac6600d4748b327281bbff258d0a76f32 pmc_34069239.pdf 41504c2d5a81fbdd260288810c3eecc42455498dce1699c4be91f5ffed773447 pmc_34069239.pdf Title: M448R and MGF505-7R: Two African Swine Fever Virus Antigens Commonly Recognized by ASFV-Specific T-Cells and with Protective Potential Subject: African swine fever (ASF) is today's number one threat for the global swine industry. Neither commercial vaccine nor treatment is available against ASF and, thus far, only live attenuated viruses (LAV) have provided robust protection against lethal ASF virus (ASFV) challenge infections. Identification of ASFV proteins inducing protective immune responses is one of the major challenges to develop safer and efficient subunit vaccines. Immunopeptidomic studies recently performed in our laboratory allowed identifying ASFV antigens recognized by ASFV-specific CD8+ T-cells. Here, we used data from the SLAI-peptide repertoire presented by a single set of ASFV-infected porcine alveolar macrophages to generate a complex DNA vaccine composed by 15 plasmids encoding the individual peptide-bearing ORFs. DNA vaccine priming improved the protection afforded by a suboptimal dose of the BA71CD2 LAV given as booster vaccination, against Georgia2007/1 lethal challenge. Interestingly, M448R was the only protein promiscuously recognized by the induced ASFV-specific T-cells. Furthermore, priming pigs with DNA plasmids encoding M488R and MGF505-7R, a CD8+ T-cell antigen previously described, confirmed these two proteins as T-cell antigens with protective potential. These studies might be useful to pave the road for designing safe and more efficient vaccine formulations in the future. Keywords: African swine fever; antigen discovery; T-cells; DNA immunization; live attenuated virus; protection; immunopeptidomics Author: Laia Bosch-Camós, Elisabet López, Javier Collado, María J. Navas, Miguel Blanco-Fuertes, Sonia Pina-Pedrero, Francesc Accensi, Maria Luisa Salas, Egbert Mundt, Veljko Nikolin and Fernando Rodríguez Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Fri May 14 16:18:08 2021 CEST ModDate: Fri May 14 16:18:08 2021 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 16 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 1679039 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LEVWDR+URWPalladioL-Ital Type 1 Custom yes yes no 123 0 VMQCRK+URWPalladioL-Bold Type 1 Custom yes yes no 124 0 YWWVQQ+VnURWPalladioL-Bold Type 1 Custom yes yes no 125 0 HUQMXY+URWPalladioL-Roma Type 1 Custom yes yes no 126 0 SDGJWK+VnURWPalladioL Type 1 Custom yes yes no 129 0 VIWIOF+CMSY10 Type 1 Builtin yes yes no 130 0 MPUDFL+PazoMath Type 1 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CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはの〠 Item: Title: きちぴづひどちぬび〠ちのつ〠きづぴとはつび〠 Children: Item: Title: ぃづぬぬび〠 Item: Title: ぁてひどっちの〠こぷどのづ〠うづぶづひ〠ざどひふび〠こぴひちどのび〠 Item: Title: ぉつづのぴどてどっちぴどはの〠はて〠こがぁぉ〭あはふのつ〠ぐづばぴどつづび〠 Item: Title: ぐぬちびねどつび〠ぅのっはつどので〠ぁこうざ〠ぇづのづび〠 Item: Title: ぉの〠ざどぶは〠ぅへばづひどねづのぴちぬ〠いづびどでの〠 Item: Title: ぁのちぬべぴどっちぬ〠ぁびびちべび〠 Item: Title: こぴちぴどびぴどっちぬ〠ぁのちぬべびどび〠 Item: Title: げづびふぬぴび〠 Children: Item: Title: いぎぁ〠ぉねねふのどぺちぴどはの〠ぷどぴと〠ち〠ぃはっにぴちどぬ〠はて〠ぐぬちびねどつび〠ぅのっはつどので〠〱〵〠ぁこうざ〠ぐひづ〭こづぬづっぴづつ〠ぐひはぴづどのび〠ぉねばひはぶづび〠ぐひはぴづっぴどはの〠ちでちどのびぴ〠ぇづはひでどち〲〰〰〷〯〱〠ぃとちぬぬづのでづ〠ぉのてづっぴどはの〠 Item: Title: ぉねねふのどぺちぴどはの〠ぷどぴと〠ぴとづ〠〱〵〠ぐぬちびねどつび〠ぉのつふっづび〠ぁこうざ〭こばづっどてどっ〠ご〭ぃづぬぬび〠ごとちぴ〠こばづっどてどっちぬぬべ〠げづっはでのどぺづ〠き〴〸〸げ〠 Item: Title: いぎぁ〠ぉねねふのどぺちぴどはの〠ぷどぴと〠ばぃきざ〭さぢ〭き〴〴〸げ〠ちのつ〠ばぃきざ〭さぢ〭きぇう〵〰〵〭〷げ〠ぉねばひはぶづび〠ぐひはぴづっぴどはの〠ちでちどのびぴ〠ぇづはひでどち〲〰〰〷〯〱〠がづぴとちぬ〠ぃとちぬぬづのでづ〠 Item: Title: き〴〴〸げ〠ちのつ〠きぇう〵〰〵〭〷げ〠ぁひづ〠うひづぱふづのぴぬべ〠げづっはでのどぺづつ〠ぢべ〠ぁこうざ〭こばづっどてどっ〠ご〭ぃづぬぬび〠 Item: Title: いどびっふびびどはの〠 Item: Title: げづてづひづのっづび Filters: FilterPipeline: 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309, 353, 346, 364 Annotation: Subtype: Link Rect: 377, 330, 415, 341 Annotation: Subtype: Link Rect: 421, 330, 457, 341 Annotation: Subtype: Link Rect: 115, 308, 152, 318 Annotation: Subtype: Link Rect: 365, 284, 402, 295 Annotation: Subtype: Link Rect: 149, 262, 186, 272 Annotation: Subtype: Link Rect: 473, 238, 511, 249 Annotation: Subtype: Link Rect: 517, 238, 553, 249 Checksum: c2a4b0bf Type: CRC32 Checksum: d7cd410d32d099b5d2a7129d27fcc10a Type: MD5 Checksum: 08153bcac6600d4748b327281bbff258d0a76f32 Type: SHA-1 Checksum: 41504c2d5a81fbdd260288810c3eecc42455498dce1699c4be91f5ffed773447 Type: SHA-256