efdd58ac969e952121ed1681a4bdc480 pmc_34069250.pdf b76309ededcdc66404bcfeb515040efd012304d3 pmc_34069250.pdf f1571f9bfe00c344cc498f48d9d500da980b2c3a332b3dcc5b893a4aa215aa4e pmc_34069250.pdf Title: Altered Nasal Microbiota Composition Associated with Development of Polyserositis by Mycoplasma hyorhinis Subject: Fibrinous polyserositis in swine farming is a common pathological finding in nursery animals. The differential diagnosis of this finding should include Glaesserella parasuis (aetiological agent of Glässer’s disease) and Mycoplasma hyorhinis, among others. These microorganisms are early colonizers of the upper respiratory tract of piglets. The composition of the nasal microbiota at weaning was shown to constitute a predisposing factor for the development of Glässer’s disease. Here, we unravel the role of the nasal microbiota in the subsequent systemic infection by M. hyorhinis, and the similarities and differences with Glässer’s disease. Nasal samples from farms with recurrent problems with polyserositis associated with M. hyorhinis (MH) or Glässer’s disease (GD) were included in this study, together with healthy control farms (HC). Nasal swabs were taken from piglets in MH farms at weaning, before the onset of the clinical outbreaks, and were submitted to 16S rRNA gene amplicon sequencing (V3–V4 region). These sequences were analyzed together with sequences from similar samples previously obtained in GD and HC farms. Animals from farms with disease (MH and GD) had a nasal microbiota with lower diversity than those from the HC farms. However, the composition of the nasal microbiota of the piglets from these disease farms was different, suggesting that divergent microbiota imbalances may predispose the animals to the two systemic infections. We also found variants of the pathogens that were associated with the farms with the corresponding disease, highlighting the importance of studying the microbiome at strain-level resolution. Keywords: porcine polyserositis; nasal microbiota; Mycoplasma hyorhinis; microbial diversity; 16S rRNA gene; Glässer’s disease Author: Miguel Blanco-Fuertes, Florencia Correa-Fiz, Lorenzo Fraile, Marina Sibila and Virginia Aragon Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Fri May 14 14:43:31 2021 CEST ModDate: Fri May 14 14:43:31 2021 CEST Custom Metadata: yes Metadata Stream: no Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 11 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 12514812 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- SPXSUG+URWPalladioL-Ital Type 1 Custom yes yes no 90 0 FMVLLG+URWPalladioL-Bold Type 1 Custom yes yes no 91 0 DVOARE+URWPalladioL-BoldItal Type 1 Custom yes yes no 92 0 WENWUI+URWPalladioL-Roma Type 1 Custom yes yes no 93 0 GXWHUJ+VnURWPalladioL Type 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