8d5e202c1a3f91a7afd487a6089551cb pmc_34069888.pdf c22549ab88e08012e92fe85303c7314679ae4d96 pmc_34069888.pdf 22ab9768ef95aa42d73877cf05fc901390523ff18cf690164de516523842a77f pmc_34069888.pdf Title: A Specific Tubular ApoA-I Distribution Is Associated to FSGS Recurrence after Kidney Transplantation Subject: A major complication of primary focal segmental glomerulosclerosis (FSGS) is its recurrence after kidney transplantation that happens in 30 to 40% of the patients. The diagnosis of these relapses is not always easy as the histological lesions are not highly specific and appear after the proteinuria increase. Currently, there are no accurate biomarkers to detect FSGS recurrence. Our group identified a modified form of Apolipoprotein A-I (ApoA-I), named ApoA-Ib, specifically present in the urine of recurrent FSGS patients after kidney transplantation. Aberrant forms of ApoA-I have also been described in the urine of native primary FSGS patients; this feature has been associated with prominent staining of ApoA-I at the apical membrane of the tubular cells. In this study, we aim to analyze the ApoA-I distribution in kidney allograft biopsies of recurrent FSGS patients. We detected ApoA-I by immunohistochemistry in kidney allograft biopsies of patients with FSGS relapse after kidney transplantation and in kidney allograft biopsies of patients with a disease different from FSGS in the native kidney (non-FSGS). In recurrent FSGS patients, ApoA-I was prominently localized at the brush border of the tubular cells, while in the non-FSGS patients, ApoA-I was found along the cytoplasm of the tubular cells. The localization of ApoA-I at the brush border of the tubular cells is a specific feature of primary FSGS in relapse. This suggests that ApoA-I staining in kidney biopsies, coupled with ApoA-Ib measurement in urine, could be used as a diagnostic tool of primary FSGS relapse after kidney transplantation due to its highly specific tubular distribution. Keywords: focal segmental glomerulosclerosis; FSGS; recurrence; apolipoprotein A-I; apolipoprotein A-Ib; ApoA-I; ApoA-Ib; biomarkers; glomerular disease; kidney transplantation Author: Conxita Jacobs-Cachá, Natàlia Puig-Gay, Ander Vergara, Maria-Alejandra Gabaldon, Joana Sellarés, Yolanda Villena-Ortiz, Irene Agraz, Francesc Moreso, Maria José Soler, Daniel Serón and Joan López-Hellín Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Tue May 18 10:48:54 2021 CEST ModDate: Tue May 18 10:48:54 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 9 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2727420 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- NRUAZV+URWPalladioL-Ital Type 1 Custom yes yes no 104 0 MQAVEK+URWPalladioL-Bold Type 1 Custom yes yes no 105 0 JUXSQN+VnURWPalladioL-Bold Type 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EDBCFE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 226 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 03:13:33 CEST RepresentationInformation: pmc_34069888.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-06-07 03:34:56 CEST Size: 2727420 Format: PDF Version: 1.5 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 0 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: ViewerPreferences: HideToolbar: false HideMenubar: false HideWindowUI: false FitWindow: true CenterWindow: false DisplayDocTitle: false NonFullScreenPageMode: UseNone Direction: L2R ViewArea: CropBox ViewClip: CropBox PrintArea: CropBox PageClip: CropBox PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: ぉのぴひはつふっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸲ Item: Title: ぅへばづひどねづのぴちぬぜ〴ぜ〰こづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮ㄩ‾㸊⡜㌷㙜㌷㝜〰ぐちぴどづのぴびぜ〴ぜ〰ちのつぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰さひどのちひべぜ〴ぜ〰ぁばはぁ〭ぉぢぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはつづぴづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁばはぁ〭ぉぜ〴ぜ〰どのぜ〴ぜ〰げづのちぬぜ〴ぜ〰あどはばびどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Children: Item: Title: ぐちぴどづのぴびぜ〴ぜ〰ちのつぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰さひどのちひべぜ〴ぜ〰ぁばはぁ〭ぉぢぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはつづぴづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁばはぁ〭ぉぜ〴ぜ〰どのぜ〴ぜ〰げづのちぬぜ〴ぜ〰あどはばびどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: さひどのちひべぜ〴ぜ〰ぁばはぁ〭ぉぢぜ〴ぜ〰いづぴづっぴどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰えどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはつづぴづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁばはぁ〭ぉぜ〴ぜ〰どのぜ〴ぜ〰げづのちぬぜ〴ぜ〰あどはばびどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: えどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぉねねふのはつづぴづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁばはぁ〭ぉぜ〴ぜ〰どのぜ〴ぜ〰げづのちぬぜ〴ぜ〰あどはばびどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぉねねふのはつづぴづっぴどはのぜ〴ぜ〰はてぜ〴ぜ〰ぁばはぁ〭ぉぜ〴ぜ〰どのぜ〴ぜ〰げづのちぬぜ〴ぜ〰あどはばびどづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰いづねはでひちばとどっぜ〴ぜ〰ちのつぜ〴ぜ〰あちびづぬどのづぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぁばはぁ〭ぉぜ〴ぜ〰ぉびぜ〴ぜ〰がはっちぴづつぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰あひふびとぜ〴ぜ〰あはひつづひぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ごふぢふぬちひぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰はてぜ〴ぜ〰うこぇこぜ〴ぜ〰げづっふひひづのぴぜ〴ぜ〰ぐちぴどづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: いづねはでひちばとどっぜ〴ぜ〰ちのつぜ〴ぜ〰あちびづぬどのづぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰ぁばはぁ〭ぉぜ〴ぜ〰ぉびぜ〴ぜ〰がはっちぴづつぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰あひふびとぜ〴ぜ〰あはひつづひぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ごふぢふぬちひぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰はてぜ〴ぜ〰うこぇこぜ〴ぜ〰げづっふひひづのぴぜ〴ぜ〰ぐちぴどづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぁばはぁ〭ぉぜ〴ぜ〰ぉびぜ〴ぜ〰がはっちぴづつぜ〴ぜ〰はのぜ〴ぜ〰ぴとづぜ〴ぜ〰あひふびとぜ〴ぜ〰あはひつづひぜ〴ぜ〰はてぜ〴ぜ〰ぴとづぜ〴ぜ〰ごふぢふぬちひぜ〴ぜ〰ぃづぬぬびぜ〴ぜ〰はてぜ〴ぜ〰うこぇこぜ〴ぜ〰げづっふひひづのぴぜ〴ぜ〰ぐちぴどづのぴびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: NRUAZV+URWPalladioL-Ital FontSubset: true FirstChar: 45 LastChar: 121 FontDescriptor: FontName: NRUAZV+URWPalladioL-Ital Flags: Symbolic FontBBox: -170, -305, 1010, 941 FontFile: true EncodingDictionary: 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