ad8854ef6c0caa039f143e0223c1fdfc pmc_34070176.pdf 0d229fcd5314ebb03d0bd66d70160ba80f8a8799 pmc_34070176.pdf 10e32fa1581dda0b8bea85ade7d8e1a52e43578fb327a2353aa8e50064f85bd6 pmc_34070176.pdf Title: Near Infrared Reflectance Spectroscopy Analysis to Predict Diet Composition of a Mountain Ungulate Species Subject: The diet composition of ungulates is important to understand not only their impact on vegetation, but also to understand the consequences of natural and human-driven environmental changes on the foraging behavior of these mammals. In this work, we evaluated the use of near infrared reflectance spectroscopy analysis (NIRS), a quick, economic and non-destructive method, to assess the diet composition of the Pyrenean chamois Rupicapra pyrenaica pyrenaica. Fecal samples (n = 192) were collected from two chamois populations in the French and Spanish Pyrenees. Diet composition was initially assessed by fecal cuticle microhistological analysis (CMA) and categorized into four functional groups, namely: woody, herbaceous, graminoid and Fabaceae plants. Regressions of modified partial least squares and several combinations of scattering correction and derivative treatments were tested. The results showed that models based on the second derivative processing obtained the higher determination coefficient for woody, herbaceous and graminoid plants (R2CAL, coefficient of determination in calibration, ranged from 0.86 to 0.91). The Fabaceae group, however, was predicted with lower accuracy (R2CAL = 0.71). Even though an agreement between NIRS and CMA methods was confirmed by a Bland–Altman analysis, confidence limits of agreement differed by up to 25%. Our results support the viability of fecal NIRS analysis to study spatial and temporal variations of the Pyrenean chamois’ diets in summer and winter when differences in the consumption of woody and annual plants are the greatest. This new use for the NIRS technique would be useful to assess the consequences of global change on the feeding behavior of this mountain ungulate and also in other ungulate counterparts. Keywords: diet composition; fecal NIRS; foraging ecology; global change; Rupicapra pyrenaica pyrenaica Author: Laia Jarque-Bascuñana, Jordi Bartolomé, Emmanuel Serrano, Johan Espunyes, Mathieu Garel, Juan Antonio Calleja Alarcón, Jorge Ramón López-Olvera and Elena Albanell Creator: LaTeX with hyperref package Producer: pdfTeX-1.40.18 CreationDate: Wed May 19 10:56:53 2021 CEST ModDate: Wed May 19 10:56:53 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 11228111 bytes Optimized: no PDF version: 1.5 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- VZZJRO+URWPalladioL-Ital Type 1 Custom yes yes no 143 0 JLIAKQ+URWPalladioL-Bold Type 1 Custom yes yes no 144 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 145 0 EACKFN+URWPalladioL-Roma Type 1 Custom yes yes no 146 0 HKQHZQ+VnURWPalladioL 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こちねばぬどのでぜ〴ぜ〰ぁひづちぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㈩‾㸊⡜㌷㙜㌷㝜〰うづっちぬぜ〴ぜ〰こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰うづっちぬぜ〴ぜ〰ぃふぴどっぬづぜ〴ぜ〰きどっひはとどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぉげこぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰ちのつぜ〴ぜ〰こばづっぴひちぬぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどはのびとどばびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰うづっちぬぜ〴ぜ〰ぃきぁぜ〴ぜ〰ちのつぜ〴ぜ〰ぎぉげこぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: うづっちぬぜ〴ぜ〰こちねばぬどのでぜ〴ぜ〰ぐひはっづつふひづぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㌩‾㸊⡜㌷㙜㌷㝜〰うづっちぬぜ〴ぜ〰ぃふぴどっぬづぜ〴ぜ〰きどっひはとどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぉげこぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰ちのつぜ〴ぜ〰こばづっぴひちぬぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどはのびとどばびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰うづっちぬぜ〴ぜ〰ぃきぁぜ〴ぜ〰ちのつぜ〴ぜ〰ぎぉげこぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: うづっちぬぜ〴ぜ〰ぃふぴどっぬづぜ〴ぜ〰きどっひはとどびぴはぬはでどっちぬぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㐩‾㸊⡜㌷㙜㌷㝜〰ぎぉげこぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰ちのつぜ〴ぜ〰こばづっぴひちぬぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどはのびとどばびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰うづっちぬぜ〴ぜ〰ぃきぁぜ〴ぜ〰ちのつぜ〴ぜ〰ぎぉげこぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: ぎぉげこぜ〴ぜ〰ぁのちぬべびどびぜ〴ぜ〰ちのつぜ〴ぜ〰こばづっぴひちぬぜ〴ぜ〰いちぴちぜ〴ぜ〰ぁのちぬべびどびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㈮㔩‾㸊⡜㌷㙜㌷㝜〰げづぬちぴどはのびとどばびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰うづっちぬぜ〴ぜ〰ぃきぁぜ〴ぜ〰ちのつぜ〴ぜ〰ぎぉげこぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづぬちぴどはのびとどばびぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰うづっちぬぜ〴ぜ〰ぃきぁぜ〴ぜ〰ちのつぜ〴ぜ〰ぎぉげこぜ〴ぜ〰きづぴとはつびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸳ Item: Title: げづびふぬぴびぜ〴ぜ〰ちのつぜ〴ぜ〰いどびっふびびどはのぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮ㄩ‾㸊⡜㌷㙜㌷㝜〰こばづっぴひちぬぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴ぜ〰はてぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いづぶづぬはばねづのぴぜ〴ぜ〰はてぜ〴ぜ〰ぐひづつどっぴどはのぜ〴ぜ〰きはつづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはねばちひどびはのぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ぎぉげこぜ〴ぜ〰ぐひづつどっぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰きどっひはとどびぴはぬはでどっぜ〴ぜ〰きづぴとはつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Children: Item: Title: こばづっぴひちぬぜ〴ぜ〰ぃとちひちっぴづひどびぴどっびぜ〴ぜ〰はてぜ〴ぜ〰こちねばぬづびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㈩‾㸊⡜㌷㙜㌷㝜〰いづぶづぬはばねづのぴぜ〴ぜ〰はてぜ〴ぜ〰ぐひづつどっぴどはのぜ〴ぜ〰きはつづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはねばちひどびはのぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ぎぉげこぜ〴ぜ〰ぐひづつどっぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰きどっひはとどびぴはぬはでどっぜ〴ぜ〰きづぴとはつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: いづぶづぬはばねづのぴぜ〴ぜ〰はてぜ〴ぜ〰ぐひづつどっぴどはのぜ〴ぜ〰きはつづぬびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳畢獥捴楯渮㌮㌩‾㸊⡜㌷㙜㌷㝜〰ぃはねばちひどびはのぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ぎぉげこぜ〴ぜ〰ぐひづつどっぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰きどっひはとどびぴはぬはでどっぜ〴ぜ〰きづぴとはつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはねばちひどびはのぜ〴ぜ〰ぢづぴぷづづのぜ〴ぜ〰ぎぉげこぜ〴ぜ〰ぐひづつどっぴどはのびぜ〴ぜ〰ちのつぜ〴ぜ〰きどっひはとどびぴはぬはでどっぜ〴ぜ〰きづぴとはつぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⸴ Item: Title: ぃはのっぬふびどはのびぜ〴〩਼㰠⽓ 䝯呯 䐠⡳散瑩潮⹁⸩‾㸊⠩਼㰠⽓ 䝯呯 䐠⡳灥捩慬瑡扬攮捡灴楯渮㤩‾㸊⡜㌷㙜㌷㝜〰げづてづひづのっづび Item: Title: Item: Title: げづてづひづのっづび Filters: FilterPipeline: FlateDecode Fonts: Type1: Font: BaseFont: JLIAKQ+URWPalladioL-Bold FontSubset: true FirstChar: 3 LastChar: 241 FontDescriptor: FontName: JLIAKQ+URWPalladioL-Bold Flags: Symbolic FontBBox: -152, -301, 1000, 935 FontFile: true EncodingDictionary: Differences: true Font: BaseFont: NYWBCD+EURB10 FontSubset: true FirstChar: 33 LastChar: 33 FontDescriptor: FontName: NYWBCD+EURB10 Flags: Symbolic FontBBox: -30, -247, 1178, 723 FontFile: true Font: BaseFont: HJOMHV+VnURWPalladioL-Bold FontSubset: true FirstChar: 178 LastChar: 226 FontDescriptor: FontName: HJOMHV+VnURWPalladioL-Bold 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