924e91865325c2438b21faa68a2d064c pmc_34200598.pdf b00da9e872e109f0b3f6627142d31ad06ba787ab pmc_34200598.pdf ea8a0f77b67751f68a1726b10e1ea5f8d3881ffa4a8d0e65489ab23b8f302c28 pmc_34200598.pdf Title: Incorporation of 5-Bromo-2'-deoxyuridine into DNA and Proliferative Behavior of Cerebellar Neuroblasts: All That Glitters Is Not Gold Subject: The synthetic halogenated pyrimidine analog, 5-bromo-2'-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety of animals including insects, birds, and mammals. Despite this, the detrimental effects of the incorporation of BrdU into DNA on proliferation and viability of different types of cells has been frequently neglected. This review will summarize and present the effects of a pulse of BrdU, at doses ranging from 25 to 300 g/g, or repeated injections. The latter, following the method of the progressively delayed labeling comprehensive procedure. The prenatal and perinatal development of the cerebellum are studied. These current data have implications for the interpretation of the results obtained by this marker as an index of the generation, migration, and settled pattern of neurons in the developing central nervous system. Caution should be exercised when interpreting the results obtained using BrdU. This is particularly important when high or repeated doses of this agent are injected. I hope that this review sheds light on the effects of this toxic maker. It may be used as a reference for toxicologists and neurobiologists given the broad use of 5-bromo-2'-deoxyuridine to label dividing cells. Keywords: prenatal life; perinatal life; 5-bromo-2'-deoxyuridine; cell cycle; cerebellar neuroepithelium; external granular layer; neurogenetic timetables; neurogenetic gradients; apoptosis Author: Joaquín Martí-Clúa Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Thu Jun 10 13:05:57 2021 CEST ModDate: Thu Jun 10 13:26:20 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 15 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 2946575 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- LPYGPS+VnURWPalladioL-Bold Type 1 Custom yes yes no 10 0 RUHGNR+VnURWPalladioL Type 1 Custom yes yes no 15 0 OVNEXW+EURM10 Type 1 Builtin yes yes no 19 0 GMSJLI+URWPalladioL-Ital Type 1 Custom yes yes no 23 0 DFBXQT+URWPalladioL-Bold Type 1 Custom yes yes no 28 0 PXEKZD+CMBSY10 Type 1 Builtin yes yes no 32 0 GDLIEN+URWPalladioL-Roma Type 1 Custom yes yes no 36 0 ZPCLNL+CMSY10 Type 1 Builtin yes yes no 40 0 PFKOHU+TimesNewRomanPS-BoldItalicMT Type 1C WinAnsi yes yes no 48 0 MCBCCI+SourceSansRoman-Regular Type 1C Custom yes yes no 70 0 MCBCCM+SourceSansRoman.613wght Type 1C Custom yes yes no 74 0 MCBCCO+SourceSansRoman-Bold Type 1C Custom yes yes no 78 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 102 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 105 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 108 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 115 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 118 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 121 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 131 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 134 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 137 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 146 0 OGBMBC+PalatinoLinotype CID TrueType Identity-H yes yes yes 149 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 155 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 158 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 168 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 171 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 174 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 188 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 191 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 194 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 205 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 208 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 211 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 221 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 224 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 227 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 241 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 244 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 247 0 OGBMBB+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 255 0 OGBMBD+PalatinoLinotype TrueType WinAnsi yes yes no 258 0 OGBMBF+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 261 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2023-05-27 02:43:40 CEST RepresentationInformation: pmc_34200598.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2023-03-31 12:14:18 CEST Size: 2946575 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 517 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@44c8afef Item: Title: The Thymidine Analogue 5-Bromo-2'-Deoxyuridine: An Overview Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7b69c6ba Item: Title: The Cerebellum: A Model to Assess the Effects of Bromodeoxyuridine Administration Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@46daef40 Item: Title: Effect of Bromodeoxyuridine Exposure on the Cerebellar Neuroepithelium Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@12f41634 Item: Title: Effects of Bromodeoxyuridine Exposure on the Cerebellar External Granular Layer Neuroblasts Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Inferring Purkinje Cells and Deep Cerebellar Neurons Developmental Timetables with Bromodeoxyuridine or Tritiated Thymidine. What Is the Most Suitable Marker? Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Info: Title: Incorporation of 5-Bromo-2'-deoxyuridine into DNA and Proliferative Behavior of Cerebellar Neuroblasts: All That Glitters Is Not Gold Author: Joaquín Martí-Clúa Subject: The synthetic halogenated pyrimidine analog, 5-bromo-2'-deoxyuridine (BrdU), is a marker of DNA synthesis. This exogenous nucleoside has generated important insights into the cellular mechanisms of the central nervous system development in a variety of animals including insects, birds, and mammals. Despite this, the detrimental effects of the incorporation of BrdU into DNA on proliferation and viability of different types of cells has been frequently neglected. This review will summarize and present the effects of a pulse of BrdU, at doses ranging from 25 to 300 g/g, or repeated injections. The latter, following the method of the progressively delayed labeling comprehensive procedure. The prenatal and perinatal development of the cerebellum are studied. These current data have implications for the interpretation of the results obtained by this marker as an index of the generation, migration, and settled pattern of neurons in the developing central nervous system. Caution should be exercised when interpreting the results obtained using BrdU. This is particularly important when high or repeated doses of this agent are injected. I hope that this review sheds light on the effects of this toxic maker. It may be used as a reference for toxicologists and neurobiologists given the broad use of 5-bromo-2'-deoxyuridine to label dividing cells. 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