ba6c0aff0b184cd694f7945f00356afa pmc_34203063.pdf fa510ad6e84914728392bbc5cfd988bb24e07f7d pmc_34203063.pdf 96bcc5ac35caac9b7409395d5b56f87727b3b92f225780a452707bf97f11ceba pmc_34203063.pdf Title: Microrheometer for Biofluidic Analysis: Electronic Detection of the Fluid-Front Advancement Subject: The motivation for this study was to develop a microdevice for the precise rheological characterization of biofluids, especially blood. The method presented was based on the principles of rheometry and fluid mechanics at the microscale. Traditional rheometers require a considerable amount of space, are expensive, and require a large volume of sample. A mathematical model was developed that, combined with a proper experimental model, allowed us to characterize the viscosity of Newtonian and non-Newtonian fluids at different shear rates. The technology presented here is the basis of a point-of-care device capable of describing the nonlinear rheology of biofluids by the fluid/air interface front velocity characterization through a microchannel. The proposed microrheometer uses a small amount of sample to deliver fast and accurate results, without needing a large laboratory space. Blood samples from healthy donors at distinct hematocrit percentages were the non-Newtonian fluid selected for the study. Water and plasma were employed as testing Newtonian fluids for validation of the system. The viscosity results obtained for the Newtonian and non-Newtonian fluids were consistent with pertinent studies cited in this paper. In addition, the results achieved using the proposed method allowed distinguishing between blood samples with different characteristics. Keywords: rheometer; microrheometer; rheology; hemorheology; viscosity; blood; plasma Author: Lourdes Méndez-Mora, Maria Cabello-Fusarés, Josep Ferré-Torres, Carla Riera-Llobet, Samantha López, Claudia Trejo-Soto, Tomas Alarcón and Aurora Hernandez-Machado Creator: LaTeX with hyperref Producer: pdfTeX-1.40.21 CreationDate: Wed Jun 23 09:54:50 2021 CEST ModDate: Thu Jun 24 10:02:37 2021 CEST Tagged: no UserProperties: no Suspects: no Form: none JavaScript: no Pages: 20 Encrypted: no Page size: 595.276 x 841.89 pts (A4) Page rot: 0 File size: 3896013 bytes Optimized: no PDF version: 1.7 name type encoding emb sub uni object ID ------------------------------------ ----------------- ---------------- --- --- --- --------- OGLZWV+VnURWPalladioL Type 1 Custom yes yes no 10 0 XUCQFV+URWPalladioL-Ital Type 1 Custom yes yes no 15 0 ZSWWHO+URWPalladioL-Bold Type 1 Custom yes yes no 20 0 YZUNZU+URWPalladioL-Roma Type 1 Custom yes yes no 24 0 HJOMHV+VnURWPalladioL-Bold Type 1 Custom yes yes no 28 0 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no 515 0 JNLEHE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 518 0 JNLGGK+CambriaMath CID TrueType Identity-H yes yes yes 521 0 JNLHDG+Calibri CID TrueType Identity-H yes yes yes 527 0 JNLEGA+PalatinoLinotype,Bold TrueType WinAnsi yes yes no 540 0 JNLEGB+PalatinoLinotype CID TrueType Identity-H yes yes yes 543 0 JNLEGC+PalatinoLinotype TrueType WinAnsi yes yes no 549 0 JNLEHE+PalatinoLinotype,Italic TrueType WinAnsi yes yes no 552 0 JNLGGK+CambriaMath CID TrueType Identity-H yes yes yes 555 0 JNLHDG+Calibri CID TrueType Identity-H yes yes yes 561 0 CRUZTT+VnURWPalladioL-Italic Type 1 Custom yes yes no 580 0 Jhove (Rel. 1.22.1, 2019-04-17) Date: 2021-09-11 03:06:29 CEST RepresentationInformation: pmc_34203063.pdf ReportingModule: PDF-hul, Rel. 1.12.1 (2019-04-17) LastModified: 2021-07-05 03:37:04 CEST Size: 3896013 Format: PDF Version: 1.7 Status: Well-Formed and valid SignatureMatches: PDF-hul MIMEtype: application/pdf PDFMetadata: Objects: 753 FreeObjects: 1 IncrementalUpdates: 0 DocumentCatalog: PageLayout: SinglePage PageMode: UseNone Outlines: Item: Title: Introduction Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@13c27452 Item: Title: Materials and Methods Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@262b2c86 Children: Item: Title: Mathematical Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@371a67ec Item: Title: Experimental Model Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@5ed828d Children: Item: Title: General Case: Non-Newtonian Fluids Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@50d0686 Item: Title: Newtonian Fluids Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@7a3d45bd Item: Title: Experimental Method Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1e7c7811 Item: Title: Experimental Results and Discussion Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77ec78b9 Item: Title: Conclusions Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@1a3869f4 Item: Title: Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@a38d7a3 Children: Item: Title: Flow Inside a Known Geometry Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@77f99a05 Item: Title: Pressure Drop Inside a Microfluidic System Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@63440df3 Item: Title: Newtonian Fluids Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@3aeaafa6 Item: Title: Viscosity Data Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@76a3e297 Item: Title: References Destination: edu.harvard.hul.ois.jhove.module.pdf.PdfSimpleObject@4d3167f4 Info: Title: Microrheometer for Biofluidic Analysis: Electronic Detection of the Fluid-Front Advancement Author: Lourdes Méndez-Mora, Maria Cabello-Fusarés, Josep Ferré-Torres, Carla Riera-Llobet, Samantha López, Claudia Trejo-Soto, Tomas Alarcón and Aurora Hernandez-Machado Subject: The motivation for this study was to develop a microdevice for the precise rheological characterization of biofluids, especially blood. The method presented was based on the principles of rheometry and fluid mechanics at the microscale. Traditional rheometers require a considerable amount of space, are expensive, and require a large volume of sample. A mathematical model was developed that, combined with a proper experimental model, allowed us to characterize the viscosity of Newtonian and non-Newtonian fluids at different shear rates. The technology presented here is the basis of a point-of-care device capable of describing the nonlinear rheology of biofluids by the fluid/air interface front velocity characterization through a microchannel. The proposed microrheometer uses a small amount of sample to deliver fast and accurate results, without needing a large laboratory space. Blood samples from healthy donors at distinct hematocrit percentages were the non-Newtonian fluid selected for the study. Water and plasma were employed as testing Newtonian fluids for validation of the system. The viscosity results obtained for the Newtonian and non-Newtonian fluids were consistent with pertinent studies cited in this paper. In addition, the results achieved using the proposed method allowed distinguishing between blood samples with different characteristics. 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